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Mechanism of deltamethrin induced thymic and splenic toxicity in mice and its protection by piperine and curcumin: in vivo study.
Drug Chem Toxicol. 2018 Jan; 41(1):33-41.DC

Abstract

Deltamethrin (DLM) is a well-known pyrethroid insecticide which is widely used in the agriculture and home pest control due to restriction on the sale of organophosphate. DLM induced apoptosis is well known but its mechanism is still unclear. This study has been designed to find out its mechanism of apoptosis with the help of computational methods along with in vivo methods. The QikProp and ProTox results have shown that DLM has good oral absorption. The docking results reveal that DLM has a strong binding affinity toward the CD4, CD8, CD28 and CD45 receptors. Further, to understand the toxicity of DLM on lymphoid cells, a single dose of DLM (5 mg/kg, oral for seven days) has been administered to male Balb/c mice and cytotoxicity (MTT assay), oxidative stress indicators (glutathione, reactive oxygen species) and apoptotic markers (caspase-3 activity, DNA fragmentation) have been assessed in thymic and splenic single cell suspensions. Lowering of body weight, cellularity and loss in cell viability have been observed in DLM treated mice. The significant increase in ROS and GSH depletion in spleen and thymus, indicate the possible involvement of oxidative stress. The spleen cells appear more susceptible to the adverse effects of DLM than thymus cells. Further, for the amelioration of its effect, two structurally different bioactive herbal extracts, piperine and curcumin have been evaluated and have shown the cytoprotective effect by inhibiting the apoptogenic signaling pathways induced by DLM.

Authors+Show Affiliations

a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology, Mesra , Ranchi , India.a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology, Mesra , Ranchi , India.a Department of Pharmaceutical Sciences and Technology , Birla Institute of Technology, Mesra , Ranchi , India.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28633599

Citation

Kumar, Anoop, et al. "Mechanism of Deltamethrin Induced Thymic and Splenic Toxicity in Mice and Its Protection By Piperine and Curcumin: in Vivo Study." Drug and Chemical Toxicology, vol. 41, no. 1, 2018, pp. 33-41.
Kumar A, Sasmal D, Sharma N. Mechanism of deltamethrin induced thymic and splenic toxicity in mice and its protection by piperine and curcumin: in vivo study. Drug Chem Toxicol. 2018;41(1):33-41.
Kumar, A., Sasmal, D., & Sharma, N. (2018). Mechanism of deltamethrin induced thymic and splenic toxicity in mice and its protection by piperine and curcumin: in vivo study. Drug and Chemical Toxicology, 41(1), 33-41. https://doi.org/10.1080/01480545.2017.1286352
Kumar A, Sasmal D, Sharma N. Mechanism of Deltamethrin Induced Thymic and Splenic Toxicity in Mice and Its Protection By Piperine and Curcumin: in Vivo Study. Drug Chem Toxicol. 2018;41(1):33-41. PubMed PMID: 28633599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of deltamethrin induced thymic and splenic toxicity in mice and its protection by piperine and curcumin: in vivo study. AU - Kumar,Anoop, AU - Sasmal,Dinakar, AU - Sharma,Neelima, Y1 - 2017/02/28/ PY - 2017/6/22/pubmed PY - 2018/7/17/medline PY - 2017/6/22/entrez KW - Deltamethrin KW - apoptosis KW - curcumin KW - oxidative stress KW - piperine SP - 33 EP - 41 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 41 IS - 1 N2 - Deltamethrin (DLM) is a well-known pyrethroid insecticide which is widely used in the agriculture and home pest control due to restriction on the sale of organophosphate. DLM induced apoptosis is well known but its mechanism is still unclear. This study has been designed to find out its mechanism of apoptosis with the help of computational methods along with in vivo methods. The QikProp and ProTox results have shown that DLM has good oral absorption. The docking results reveal that DLM has a strong binding affinity toward the CD4, CD8, CD28 and CD45 receptors. Further, to understand the toxicity of DLM on lymphoid cells, a single dose of DLM (5 mg/kg, oral for seven days) has been administered to male Balb/c mice and cytotoxicity (MTT assay), oxidative stress indicators (glutathione, reactive oxygen species) and apoptotic markers (caspase-3 activity, DNA fragmentation) have been assessed in thymic and splenic single cell suspensions. Lowering of body weight, cellularity and loss in cell viability have been observed in DLM treated mice. The significant increase in ROS and GSH depletion in spleen and thymus, indicate the possible involvement of oxidative stress. The spleen cells appear more susceptible to the adverse effects of DLM than thymus cells. Further, for the amelioration of its effect, two structurally different bioactive herbal extracts, piperine and curcumin have been evaluated and have shown the cytoprotective effect by inhibiting the apoptogenic signaling pathways induced by DLM. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/28633599/Mechanism_of_deltamethrin_induced_thymic_and_splenic_toxicity_in_mice_and_its_protection_by_piperine_and_curcumin:_in_vivo_study_ L2 - http://www.tandfonline.com/doi/full/10.1080/01480545.2017.1286352 DB - PRIME DP - Unbound Medicine ER -