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Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield per Spike and Heading Time in Durum Wheat.
Int J Mol Sci. 2017 Jun 21; 18(6)IJ

Abstract

High-density genetic linkage maps of crop species are particularly useful in detecting qualitative and quantitative trait loci for important agronomic traits and in improving the power of classical approaches to identify candidate genes. The aim of this study was to develop a high-density genetic linkage map in a durum wheat recombinant inbred lines population (RIL) derived from two elite wheat cultivars and to identify, characterize and correlate Quantitative Trait Loci (QTL) for β-glucan, protein content, grain yield per spike and heading time. A dense map constructed by genotyping the RIL population with the wheat 90K iSelect array included 5444 single nucleotide polymorphism (SNP) markers distributed in 36 linkage groups. Data for β-glucan and protein content, grain yield per spike and heading time were obtained from replicated trials conducted at two locations in southern Italy. A total of 19 QTL were detected in different chromosome regions. In particular, three QTL for β-glucan content were detected on chromosomes 2A and 2B (two loci); eight QTL controlling grain protein content were detected on chromosomes 1B, 2B, 3B (two loci), 4A, 5A, 7A and 7B; seven QTL for grain yield per spike were identified on chromosomes 1A, 2B, 3A (two loci), 3B (two loci) and 6B; and one marker-trait association was detected on chromosome 2A for heading time. The last was co-located with a β-glucan QTL, and the two QTL appeared to be negatively correlated. A genome scan for genomic regions controlling the traits and SNP annotated sequences identified five putative candidate genes involved in different biosynthesis pathways (β-glucosidase, GLU1a; APETALA2, TaAP2; gigantea3, TaGI3; 14-3-3 protein, Ta14A; and photoperiod sensitivity, Ppd-A1). This study provides additional information on QTL for important agronomic traits that could be useful for marker-assisted breeding to obtain new genotypes with commercial and nutritional relevance.

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Authors+Show Affiliations

Marcotuli I
Department of Agricultural and Environmental Science, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. i.marcotuli@gmail.com.
Gadaleta A
Department of Agricultural and Environmental Science, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. agata.gadaleta@uniba.it.
Mangini G
Department of Soil, Plant and Food Sciences, Section of Genetic and Plant Breeding, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. giacomo.mangini@uniba.it.
Signorile AM
Department of Soil, Plant and Food Sciences, Section of Genetic and Plant Breeding, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. massimoantonio.signorile@uniba.it.
Zacheo SA
Department of Soil, Plant and Food Sciences, Section of Genetic and Plant Breeding, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. silvanaaddolorata.zacheo@uniba.it.
Blanco A
Department of Soil, Plant and Food Sciences, Section of Genetic and Plant Breeding, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. blanco17145@gmail.com.
Simeone R
Department of Soil, Plant and Food Sciences, Section of Genetic and Plant Breeding, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. rosanna.simeone@uniba.it.
Colasuonno P
Department of Agricultural and Environmental Science, University of Bari "Aldo Moro", Via G. Amendola 165/A, 70126 Bari, Italy. pattybiotec@yahoo.it.

MeSH

Edible GrainGenetic LinkageGenome, PlantGenotypePlant BreedingPlant ProteinsPolymorphism, Single NucleotideQuantitative Trait LociTriticumbeta-Glucans

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28635630

Citation

Marcotuli, Ilaria, et al. "Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield Per Spike and Heading Time in Durum Wheat." International Journal of Molecular Sciences, vol. 18, no. 6, 2017.
Marcotuli I, Gadaleta A, Mangini G, et al. Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield per Spike and Heading Time in Durum Wheat. Int J Mol Sci. 2017;18(6).
Marcotuli, I., Gadaleta, A., Mangini, G., Signorile, A. M., Zacheo, S. A., Blanco, A., Simeone, R., & Colasuonno, P. (2017). Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield per Spike and Heading Time in Durum Wheat. International Journal of Molecular Sciences, 18(6). https://doi.org/10.3390/ijms18061329
Marcotuli I, et al. Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield Per Spike and Heading Time in Durum Wheat. Int J Mol Sci. 2017 Jun 21;18(6) PubMed PMID: 28635630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a High-Density SNP-Based Linkage Map and Detection of QTL for β-Glucans, Protein Content, Grain Yield per Spike and Heading Time in Durum Wheat. AU - Marcotuli,Ilaria, AU - Gadaleta,Agata, AU - Mangini,Giacomo, AU - Signorile,Antonio Massimo, AU - Zacheo,Silvana Addolorata, AU - Blanco,Antonio, AU - Simeone,Rosanna, AU - Colasuonno,Pasqualina, Y1 - 2017/06/21/ PY - 2017/05/11/received PY - 2017/06/16/revised PY - 2017/06/17/accepted PY - 2017/6/22/entrez PY - 2017/6/22/pubmed PY - 2018/3/24/medline KW - QTL KW - durum wheat KW - grain protein content KW - grain yield per spike KW - heading time KW - single nucleotide polymorphism (SNP) mapping KW - β-glucans JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 6 N2 - High-density genetic linkage maps of crop species are particularly useful in detecting qualitative and quantitative trait loci for important agronomic traits and in improving the power of classical approaches to identify candidate genes. The aim of this study was to develop a high-density genetic linkage map in a durum wheat recombinant inbred lines population (RIL) derived from two elite wheat cultivars and to identify, characterize and correlate Quantitative Trait Loci (QTL) for β-glucan, protein content, grain yield per spike and heading time. A dense map constructed by genotyping the RIL population with the wheat 90K iSelect array included 5444 single nucleotide polymorphism (SNP) markers distributed in 36 linkage groups. Data for β-glucan and protein content, grain yield per spike and heading time were obtained from replicated trials conducted at two locations in southern Italy. A total of 19 QTL were detected in different chromosome regions. In particular, three QTL for β-glucan content were detected on chromosomes 2A and 2B (two loci); eight QTL controlling grain protein content were detected on chromosomes 1B, 2B, 3B (two loci), 4A, 5A, 7A and 7B; seven QTL for grain yield per spike were identified on chromosomes 1A, 2B, 3A (two loci), 3B (two loci) and 6B; and one marker-trait association was detected on chromosome 2A for heading time. The last was co-located with a β-glucan QTL, and the two QTL appeared to be negatively correlated. A genome scan for genomic regions controlling the traits and SNP annotated sequences identified five putative candidate genes involved in different biosynthesis pathways (β-glucosidase, GLU1a; APETALA2, TaAP2; gigantea3, TaGI3; 14-3-3 protein, Ta14A; and photoperiod sensitivity, Ppd-A1). This study provides additional information on QTL for important agronomic traits that could be useful for marker-assisted breeding to obtain new genotypes with commercial and nutritional relevance. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28635630/Development_of_a_High_Density_SNP_Based_Linkage_Map_and_Detection_of_QTL_for_β_Glucans_Protein_Content_Grain_Yield_per_Spike_and_Heading_Time_in_Durum_Wheat_ L2 - https://www.mdpi.com/resolver?pii=ijms18061329 DB - PRIME DP - Unbound Medicine ER -