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Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids.
Genome. 2017 Aug; 60(8):687-694.G

Abstract

It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R-E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.

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Authors+Show Affiliations

Dai Y
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China. b Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Duan Y
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China.
Chi D
b Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Liu H
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China. b Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Huang S
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China.
Cao W
b Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Gao Y
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China. c Jiangsu Key Laboratories of Crop Genetics and Physiology and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Fedak G
b Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada.
Chen J
a College of Bioscience and Biotechnology, Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 88 Da Xue South Road, Yangzhou 225100, Jiangsu, China. c Jiangsu Key Laboratories of Crop Genetics and Physiology and Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China.

MeSH

Chromosomes, PlantGenetic MarkersHybridization, GeneticIn Situ Hybridization, FluorescenceSecaleTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28636827

Citation

Dai, Yi, et al. "Chromosome Identification By New Molecular Markers and Genomic in Situ Hybridization in the Triticum-Secale-Thinopyrum Trigeneric Hybrids." Genome, vol. 60, no. 8, 2017, pp. 687-694.
Dai Y, Duan Y, Chi D, et al. Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids. Genome. 2017;60(8):687-694.
Dai, Y., Duan, Y., Chi, D., Liu, H., Huang, S., Cao, W., Gao, Y., Fedak, G., & Chen, J. (2017). Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids. Genome, 60(8), 687-694. https://doi.org/10.1139/gen-2017-0025
Dai Y, et al. Chromosome Identification By New Molecular Markers and Genomic in Situ Hybridization in the Triticum-Secale-Thinopyrum Trigeneric Hybrids. Genome. 2017;60(8):687-694. PubMed PMID: 28636827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids. AU - Dai,Yi, AU - Duan,Yamei, AU - Chi,Dawn, AU - Liu,Huiping, AU - Huang,Shuai, AU - Cao,Wenguang, AU - Gao,Yong, AU - Fedak,George, AU - Chen,Jianmin, Y1 - 2017/06/21/ PY - 2017/6/22/pubmed PY - 2018/4/18/medline PY - 2017/6/22/entrez KW - GISH KW - Thinopyrum elongatum KW - chromosome identification KW - chromosome-specific marker KW - identification des chromosomes KW - marqueur spécifique d’un chromosome KW - triticale SP - 687 EP - 694 JF - Genome JO - Genome VL - 60 IS - 8 N2 - It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R-E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/28636827/Chromosome_identification_by_new_molecular_markers_and_genomic_in_situ_hybridization_in_the_Triticum_Secale_Thinopyrum_trigeneric_hybrids_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2017-0025?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -