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KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES.

Abstract

BACKGROUND

The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus.

MATERIALS AND METHODS

This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro. The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited.

RESULTS

The data obtained revealed that the extract moderately and potently inhibited the specific activities of α-amylase and α-glucosidase, respectively. The inhibition was concentration-related with respective IC50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents.

CONCLUSION

The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α-amylase and α-glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.

Authors+Show Affiliations

Phytomedicine, Food Factors and Toxicology Research Laboratory, Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, Nigeria. Phytomedicine and Phytopharmacology Research Group, Plant Sciences Department, University of the Free State, Qwaqwa campus, Phuthaditjhaba 9866, South Africa.Phytomedicine, Food Factors and Toxicology Research Laboratory, Biochemistry Unit, Department of Biosciences and Biotechnology, Kwara State University, Malete, Nigeria.Plant and Environmental Biochemistry Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria.Phytomedicine and Phytopharmacology Research Group, Plant Sciences Department, University of the Free State, Qwaqwa campus, Phuthaditjhaba 9866, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28638866

Citation

Sabiu, Saheed, et al. "KINETICS of MODULATORY ROLE of Cyperus Esculentus L. ON the SPECIFIC ACTIVITY of KEY CARBOHYDRATE METABOLIZING ENZYMES." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 14, no. 4, 2017, pp. 46-53.
Sabiu S, Ajani EO, Sunmonu TO, et al. KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES. Afr J Tradit Complement Altern Med. 2017;14(4):46-53.
Sabiu, S., Ajani, E. O., Sunmonu, T. O., & Ashafa, A. O. T. (2017). KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 14(4), 46-53. https://doi.org/10.21010/ajtcam.v14i4.6
Sabiu S, et al. KINETICS of MODULATORY ROLE of Cyperus Esculentus L. ON the SPECIFIC ACTIVITY of KEY CARBOHYDRATE METABOLIZING ENZYMES. Afr J Tradit Complement Altern Med. 2017;14(4):46-53. PubMed PMID: 28638866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES. AU - Sabiu,Saheed, AU - Ajani,Emmanuel Oladipo, AU - Sunmonu,Taofik Olatunde, AU - Ashafa,Anofi Omotayo Tom, Y1 - 2017/06/05/ PY - 2017/6/23/entrez PY - 2017/6/24/pubmed PY - 2017/8/18/medline KW - Acarbose KW - Antioxidant KW - Diabetes KW - Enzyme kinetics KW - Vmax SP - 46 EP - 53 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 14 IS - 4 N2 - BACKGROUND: The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. MATERIALS AND METHODS: This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro. The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. RESULTS: The data obtained revealed that the extract moderately and potently inhibited the specific activities of α-amylase and α-glucosidase, respectively. The inhibition was concentration-related with respective IC50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. CONCLUSION: The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α-amylase and α-glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/28638866/KINETICS_OF_MODULATORY_ROLE_OF_Cyperus_esculentus_L__ON_THE_SPECIFIC_ACTIVITY_OF_KEY_CARBOHYDRATE_METABOLIZING_ENZYMES_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28638866/ DB - PRIME DP - Unbound Medicine ER -