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The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine.
Curr Protoc Pharmacol 2017; 77:7.17.1-7.17.19CP

Abstract

The Ussing chamber is an old but still powerful technique originally designed to study the vectorial transport of ions through frog skin. This technique is also used to investigate the transport of chemical agents through the intestinal barrier as well as drug metabolism in enterocytes, both of which are key determinants for the bioavailability of orally administered drugs. More contemporary model systems, such as Caco-2 cell monolayers or stably transfected cells, are more limited in their use compared to the Ussing chamber because of differences in expression rates of transporter proteins and/or metabolizing enzymes. While there are limitations to the Ussing chamber assay, the use of human intestinal tissue remains the best laboratory test for characterizing the transport and metabolism of compounds following oral administration. Detailed in this unit is a step-by-step protocol for preparing human intestinal tissue, for designing Ussing chamber experiments, and for analyzing and interpreting the findings. © 2017 by John Wiley & Sons, Inc.

Authors+Show Affiliations

Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald, Greifswald, Germany.Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald, Greifswald, Germany.AstraZeneca R&D, Mölndal, Gothenburg, Sweden.Department of General Surgery, Visceral, Thoracic and Vascular Surgery, University Medicine Greifswald, Greifswald, Germany.Department of General Surgery, Visceral, Thoracic and Vascular Surgery, University Medicine Greifswald, Greifswald, Germany.AstraZeneca R&D, Mölndal, Gothenburg, Sweden.Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald, Greifswald, Germany.Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine Greifswald, Greifswald, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28640954

Citation

Kisser, Beatrice, et al. "The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine." Current Protocols in Pharmacology, vol. 77, 2017, pp. 7.17.1-7.17.19.
Kisser B, Mangelsen E, Wingolf C, et al. The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine. Curr Protoc Pharmacol. 2017;77:7.17.1-7.17.19.
Kisser, B., Mangelsen, E., Wingolf, C., Partecke, L. I., Heidecke, C. D., Tannergren, C., ... Keiser, M. (2017). The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine. Current Protocols in Pharmacology, 77, pp. 7.17.1-7.17.19. doi:10.1002/cpph.22.
Kisser B, et al. The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine. Curr Protoc Pharmacol. 2017 Jun 22;77:7.17.1-7.17.19. PubMed PMID: 28640954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Ussing Chamber Assay to Study Drug Metabolism and Transport in the Human Intestine. AU - Kisser,Beatrice, AU - Mangelsen,Eva, AU - Wingolf,Caroline, AU - Partecke,Lars Ivo, AU - Heidecke,Claus-Dieter, AU - Tannergren,Christer, AU - Oswald,Stefan, AU - Keiser,Markus, Y1 - 2017/06/22/ PY - 2017/6/23/entrez PY - 2017/6/24/pubmed PY - 2017/7/15/medline KW - Papp-value KW - human intestine KW - in vitro KW - metabolizing enzymes KW - transporter protein KW - ussing chamber SP - 7.17.1 EP - 7.17.19 JF - Current protocols in pharmacology JO - Curr Protoc Pharmacol VL - 77 N2 - The Ussing chamber is an old but still powerful technique originally designed to study the vectorial transport of ions through frog skin. This technique is also used to investigate the transport of chemical agents through the intestinal barrier as well as drug metabolism in enterocytes, both of which are key determinants for the bioavailability of orally administered drugs. More contemporary model systems, such as Caco-2 cell monolayers or stably transfected cells, are more limited in their use compared to the Ussing chamber because of differences in expression rates of transporter proteins and/or metabolizing enzymes. While there are limitations to the Ussing chamber assay, the use of human intestinal tissue remains the best laboratory test for characterizing the transport and metabolism of compounds following oral administration. Detailed in this unit is a step-by-step protocol for preparing human intestinal tissue, for designing Ussing chamber experiments, and for analyzing and interpreting the findings. © 2017 by John Wiley & Sons, Inc. SN - 1934-8290 UR - https://www.unboundmedicine.com/medline/citation/28640954/The_Ussing_Chamber_Assay_to_Study_Drug_Metabolism_and_Transport_in_the_Human_Intestine_ L2 - https://dx.doi.org/10.1002/cpph.22 DB - PRIME DP - Unbound Medicine ER -