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Activation of the PI3K-Akt pathway promotes neuroprotection of the δ-opioid receptor agonist against cerebral ischemia-reperfusion injury in rat models.
Biomed Pharmacother. 2017 Sep; 93:230-237.BP

Abstract

The central objective was to identify the role of the PI3K-Akt activation pathway on the neuroprotection of δ-opioid receptor agonist (DADLE) against cerebral ischemia-reperfusion (I/R) injury in a rat model. Fifty-five male Sprague-Dawley (SD) rats were included to establish a middle cerebral artery occlusion (MCAO) model which were then divided into the sham, MCAO, LY294002 (MCAO+DADLE+LY294002 [inhibitor of PI3K-Akt pathway]), DADLE (MCAO+DADLE) and DMSO (MCAO+DADLE+DMSO [dimethyl sulphoxide]) groups. The cerebral infarction (CI) volume and nerve cell apoptosis was determined using TTC and TUNEL staining. Quantitative real-time polymerase chain reaction (qRT-PCR), western blotting and immunohistochemistry staining were applied for the expressions of Bad, Bax, Bcl-2 and cleaved caspase-3. The MCAO group showed higher CI volume, nerve cell apoptosis and cleaved caspase-3 expressions than the DADLE and DMSO groups, which were also higher in the LY294002 group than the DADLE group. Compared with the MCAO group, the mRNA and protein expressions of PI3K and Bcl-2, and the protein expressions of p-Akt and p-Bad were elevated, while the mRNA and protein expressions of Bax were decreased in the DADLE and DMSO groups. Decreased mRNA and protein expressions of PI3K and Bcl-2, reduced protein expressions of p-Akt and p-Bad and elevated mRNA and protein expressions of Bax exhibited in the LY294002 group than the DADLE group. These results indicate that activation of PI3K-Akt pathway promotes the neuroprotection of DADLE against cerebral I/R injury in a rat model by decreasing nerve cells apoptosis.

Authors+Show Affiliations

Department of Nursing, Linyi People's Hospital, Linyi 276003, PR China.Department of Endocrinology, Linyi People's Hospital, Linyi 276003, PR China.Department of Neurosurgery, Linyi People's Hospital, Linyi 276003, PR China.Department of Neurosurgery, Linyi People's Hospital, Linyi 276003, PR China.Department of Neurosurgery, Linyi People's Hospital, Linyi 276003, PR China.Department of Neurosurgery, Linyi People's Hospital, Linyi 276003, PR China.Outpatient Operating Room, Linyi People's Hospital, Linyi 276003, PR China. Electronic address: yeli_YY@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28645007

Citation

Lv, Mei-Rong, et al. "Activation of the PI3K-Akt Pathway Promotes Neuroprotection of the Δ-opioid Receptor Agonist Against Cerebral Ischemia-reperfusion Injury in Rat Models." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 93, 2017, pp. 230-237.
Lv MR, Li B, Wang MG, et al. Activation of the PI3K-Akt pathway promotes neuroprotection of the δ-opioid receptor agonist against cerebral ischemia-reperfusion injury in rat models. Biomed Pharmacother. 2017;93:230-237.
Lv, M. R., Li, B., Wang, M. G., Meng, F. G., Yu, J. J., Guo, F., & Li, Y. (2017). Activation of the PI3K-Akt pathway promotes neuroprotection of the δ-opioid receptor agonist against cerebral ischemia-reperfusion injury in rat models. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 93, 230-237. https://doi.org/10.1016/j.biopha.2017.05.121
Lv MR, et al. Activation of the PI3K-Akt Pathway Promotes Neuroprotection of the Δ-opioid Receptor Agonist Against Cerebral Ischemia-reperfusion Injury in Rat Models. Biomed Pharmacother. 2017;93:230-237. PubMed PMID: 28645007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the PI3K-Akt pathway promotes neuroprotection of the δ-opioid receptor agonist against cerebral ischemia-reperfusion injury in rat models. AU - Lv,Mei-Rong, AU - Li,Bin, AU - Wang,Ming-Guang, AU - Meng,Fan-Guo, AU - Yu,Jian-Jun, AU - Guo,Feng, AU - Li,Ye, Y1 - 2017/06/20/ PY - 2017/02/23/received PY - 2017/05/13/revised PY - 2017/05/25/accepted PY - 2017/6/24/pubmed PY - 2018/4/25/medline PY - 2017/6/24/entrez KW - Cerebral ischemia-reperfusion injury KW - Neuroprotection KW - Phosphatidylinositol 3-kinase-Akt pathway (PI3K-Akt) KW - Rat model KW - δ-Opioid receptor agonist SP - 230 EP - 237 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 93 N2 - The central objective was to identify the role of the PI3K-Akt activation pathway on the neuroprotection of δ-opioid receptor agonist (DADLE) against cerebral ischemia-reperfusion (I/R) injury in a rat model. Fifty-five male Sprague-Dawley (SD) rats were included to establish a middle cerebral artery occlusion (MCAO) model which were then divided into the sham, MCAO, LY294002 (MCAO+DADLE+LY294002 [inhibitor of PI3K-Akt pathway]), DADLE (MCAO+DADLE) and DMSO (MCAO+DADLE+DMSO [dimethyl sulphoxide]) groups. The cerebral infarction (CI) volume and nerve cell apoptosis was determined using TTC and TUNEL staining. Quantitative real-time polymerase chain reaction (qRT-PCR), western blotting and immunohistochemistry staining were applied for the expressions of Bad, Bax, Bcl-2 and cleaved caspase-3. The MCAO group showed higher CI volume, nerve cell apoptosis and cleaved caspase-3 expressions than the DADLE and DMSO groups, which were also higher in the LY294002 group than the DADLE group. Compared with the MCAO group, the mRNA and protein expressions of PI3K and Bcl-2, and the protein expressions of p-Akt and p-Bad were elevated, while the mRNA and protein expressions of Bax were decreased in the DADLE and DMSO groups. Decreased mRNA and protein expressions of PI3K and Bcl-2, reduced protein expressions of p-Akt and p-Bad and elevated mRNA and protein expressions of Bax exhibited in the LY294002 group than the DADLE group. These results indicate that activation of PI3K-Akt pathway promotes the neuroprotection of DADLE against cerebral I/R injury in a rat model by decreasing nerve cells apoptosis. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/28645007/Activation_of_the_PI3K_Akt_pathway_promotes_neuroprotection_of_the_δ_opioid_receptor_agonist_against_cerebral_ischemia_reperfusion_injury_in_rat_models_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)30840-5 DB - PRIME DP - Unbound Medicine ER -