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Glucose oxidase-initiated cascade catalysis for sensitive impedimetric aptasensor based on metal-organic frameworks functionalized with Pt nanoparticles and hemin/G-quadruplex as mimicking peroxidases.
Biosens Bioelectron. 2017 Dec 15; 98:83-90.BB

Abstract

Based on cascade catalysis amplification driven by glucose oxidase (GOx), a sensitive electrochemical impedimetric aptasensor for protein (carcinoembryonic antigen, CEA as tested model) was proposed by using Cu-based metal-organic frameworks functionalized with Pt nanoparticles, aptamer, hemin and GOx (Pt@CuMOFs-hGq-GOx). CEA aptamer loaded onto Pt@CuMOFs was bound with hemin to form hemin@G-quadruplex (hGq) with mimicking peroxidase activity. Through sandwich-type reaction of target CEA and CEA aptamers (Apt1 and Apt2), the obtained Pt@CuMOFs-hGq-GOx as signal transduction probes (STPs) was captured to the modified electrode interface. When 3,3-diaminobenzidine (DAB) and glucose were introduced, the cascade reaction was initiated by GOx to catalyze the oxidation of glucose, in situ generating H2O2. Simultaneously, the decomposition of the generated H2O2 was greatly promoted by Pt@CuMOFs and hGq as synergistic peroxide catalysts, accompanying with the significant oxidation process of DAB and the formation of nonconductive insoluble precipitates (IPs). As a result, the electron transfer in the resultant sensing interface was effectively hindered and the electrochemical impedimetric signal (EIS) was efficiently amplified. Thus, the high sensitivity of the proposed CEA aptasensor was successfully improved with 0.023pgmL-1, which may be promising and potential in assaying certain clinical disease related to CEA.

Authors+Show Affiliations

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: xwju@swu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28654887

Citation

Zhou, Xingxing, et al. "Glucose Oxidase-initiated Cascade Catalysis for Sensitive Impedimetric Aptasensor Based On Metal-organic Frameworks Functionalized With Pt Nanoparticles and hemin/G-quadruplex as Mimicking Peroxidases." Biosensors & Bioelectronics, vol. 98, 2017, pp. 83-90.
Zhou X, Guo S, Gao J, et al. Glucose oxidase-initiated cascade catalysis for sensitive impedimetric aptasensor based on metal-organic frameworks functionalized with Pt nanoparticles and hemin/G-quadruplex as mimicking peroxidases. Biosens Bioelectron. 2017;98:83-90.
Zhou, X., Guo, S., Gao, J., Zhao, J., Xue, S., & Xu, W. (2017). Glucose oxidase-initiated cascade catalysis for sensitive impedimetric aptasensor based on metal-organic frameworks functionalized with Pt nanoparticles and hemin/G-quadruplex as mimicking peroxidases. Biosensors & Bioelectronics, 98, 83-90. https://doi.org/10.1016/j.bios.2017.06.039
Zhou X, et al. Glucose Oxidase-initiated Cascade Catalysis for Sensitive Impedimetric Aptasensor Based On Metal-organic Frameworks Functionalized With Pt Nanoparticles and hemin/G-quadruplex as Mimicking Peroxidases. Biosens Bioelectron. 2017 Dec 15;98:83-90. PubMed PMID: 28654887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucose oxidase-initiated cascade catalysis for sensitive impedimetric aptasensor based on metal-organic frameworks functionalized with Pt nanoparticles and hemin/G-quadruplex as mimicking peroxidases. AU - Zhou,Xingxing, AU - Guo,Shijing, AU - Gao,Jiaxi, AU - Zhao,Jianmin, AU - Xue,Shuyan, AU - Xu,Wenju, Y1 - 2017/06/20/ PY - 2017/04/02/received PY - 2017/06/16/revised PY - 2017/06/19/accepted PY - 2017/6/28/pubmed PY - 2018/4/24/medline PY - 2017/6/28/entrez KW - Carcinoembryonic antigen KW - Cascade catalysis KW - EIS-based aptasensor KW - Insoluble precipitates KW - Metal-organic frameworks SP - 83 EP - 90 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 98 N2 - Based on cascade catalysis amplification driven by glucose oxidase (GOx), a sensitive electrochemical impedimetric aptasensor for protein (carcinoembryonic antigen, CEA as tested model) was proposed by using Cu-based metal-organic frameworks functionalized with Pt nanoparticles, aptamer, hemin and GOx (Pt@CuMOFs-hGq-GOx). CEA aptamer loaded onto Pt@CuMOFs was bound with hemin to form hemin@G-quadruplex (hGq) with mimicking peroxidase activity. Through sandwich-type reaction of target CEA and CEA aptamers (Apt1 and Apt2), the obtained Pt@CuMOFs-hGq-GOx as signal transduction probes (STPs) was captured to the modified electrode interface. When 3,3-diaminobenzidine (DAB) and glucose were introduced, the cascade reaction was initiated by GOx to catalyze the oxidation of glucose, in situ generating H2O2. Simultaneously, the decomposition of the generated H2O2 was greatly promoted by Pt@CuMOFs and hGq as synergistic peroxide catalysts, accompanying with the significant oxidation process of DAB and the formation of nonconductive insoluble precipitates (IPs). As a result, the electron transfer in the resultant sensing interface was effectively hindered and the electrochemical impedimetric signal (EIS) was efficiently amplified. Thus, the high sensitivity of the proposed CEA aptasensor was successfully improved with 0.023pgmL-1, which may be promising and potential in assaying certain clinical disease related to CEA. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28654887/Glucose_oxidase_initiated_cascade_catalysis_for_sensitive_impedimetric_aptasensor_based_on_metal_organic_frameworks_functionalized_with_Pt_nanoparticles_and_hemin/G_quadruplex_as_mimicking_peroxidases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30414-1 DB - PRIME DP - Unbound Medicine ER -