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Mechanical wounding promotes local and long distance response in the halophyte Cakile maritima through the involvement of the ROS and RNS metabolism.
Nitric Oxide. 2018 04 01; 74:93-101.NO

Abstract

Mechanical wounding in plants, which are capable of generating defense responses possibly associated with nitro-oxidative stress, can be caused by (a)biotic factors such as rain, wind, herbivores and insects. Sea rocket (Cakile maritima L.), a halophyte plant belonging to the mustard family Brassicaceae, is commonly found on sandy coasts throughout Europe. Using 7-day-old Cakile maritima L. seedlings, mechanical wounding was induced in hypocotyls by pinching with a striped-tip forceps; after 3 h, several biochemical parameters were analyzed in both the damaged and unwounded organs (green cotyledons and roots). We thus determined NO production, H2O2 content, lipid oxidation as well as protein nitration patterns; we also identified several antioxidant enzymes including catalase, superoxide dismutase (SOD) isozymes, peroxidases, ascorbate-glutathione cycle enzymes and NADP-dehydrogenases. All these parameters were differentially modulated in the damaged (hypocotyls) and unwounded organs, which clearly indicated an induction of CuZnSOD V in the three organs, an increase in protein nitration in green cotyledons and an induction of NADP-isocitrate dehydrogenase activity in roots. On the whole, our results indicate that the wounding of hypocotyls, which showed an active ROS metabolism and oxidative stress, causes long-distance signals that also trigger responses in unwounded tissues with a more active RNS metabolism. These data therefore confirm the existence of local and long-distance responses which counteract negative effects and provide appropriate responses, enabling the wounded seedlings to survive.

Authors+Show Affiliations

Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada, Spain. Electronic address: javier.corpas@eez.csic.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28655650

Citation

Houmani, Hayet, et al. "Mechanical Wounding Promotes Local and Long Distance Response in the Halophyte Cakile Maritima Through the Involvement of the ROS and RNS Metabolism." Nitric Oxide : Biology and Chemistry, vol. 74, 2018, pp. 93-101.
Houmani H, Rodríguez-Ruiz M, Palma JM, et al. Mechanical wounding promotes local and long distance response in the halophyte Cakile maritima through the involvement of the ROS and RNS metabolism. Nitric Oxide. 2018;74:93-101.
Houmani, H., Rodríguez-Ruiz, M., Palma, J. M., & Corpas, F. J. (2018). Mechanical wounding promotes local and long distance response in the halophyte Cakile maritima through the involvement of the ROS and RNS metabolism. Nitric Oxide : Biology and Chemistry, 74, 93-101. https://doi.org/10.1016/j.niox.2017.06.008
Houmani H, et al. Mechanical Wounding Promotes Local and Long Distance Response in the Halophyte Cakile Maritima Through the Involvement of the ROS and RNS Metabolism. Nitric Oxide. 2018 04 1;74:93-101. PubMed PMID: 28655650.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanical wounding promotes local and long distance response in the halophyte Cakile maritima through the involvement of the ROS and RNS metabolism. AU - Houmani,Hayet, AU - Rodríguez-Ruiz,Marta, AU - Palma,José M, AU - Corpas,Francisco J, Y1 - 2017/06/24/ PY - 2017/03/14/received PY - 2017/06/21/revised PY - 2017/06/22/accepted PY - 2017/6/29/pubmed PY - 2019/4/2/medline PY - 2017/6/29/entrez KW - H(2)O(2) KW - Halophyte KW - Mechanical wounding KW - Nitric oxide KW - ROS SP - 93 EP - 101 JF - Nitric oxide : biology and chemistry JO - Nitric Oxide VL - 74 N2 - Mechanical wounding in plants, which are capable of generating defense responses possibly associated with nitro-oxidative stress, can be caused by (a)biotic factors such as rain, wind, herbivores and insects. Sea rocket (Cakile maritima L.), a halophyte plant belonging to the mustard family Brassicaceae, is commonly found on sandy coasts throughout Europe. Using 7-day-old Cakile maritima L. seedlings, mechanical wounding was induced in hypocotyls by pinching with a striped-tip forceps; after 3 h, several biochemical parameters were analyzed in both the damaged and unwounded organs (green cotyledons and roots). We thus determined NO production, H2O2 content, lipid oxidation as well as protein nitration patterns; we also identified several antioxidant enzymes including catalase, superoxide dismutase (SOD) isozymes, peroxidases, ascorbate-glutathione cycle enzymes and NADP-dehydrogenases. All these parameters were differentially modulated in the damaged (hypocotyls) and unwounded organs, which clearly indicated an induction of CuZnSOD V in the three organs, an increase in protein nitration in green cotyledons and an induction of NADP-isocitrate dehydrogenase activity in roots. On the whole, our results indicate that the wounding of hypocotyls, which showed an active ROS metabolism and oxidative stress, causes long-distance signals that also trigger responses in unwounded tissues with a more active RNS metabolism. These data therefore confirm the existence of local and long-distance responses which counteract negative effects and provide appropriate responses, enabling the wounded seedlings to survive. SN - 1089-8611 UR - https://www.unboundmedicine.com/medline/citation/28655650/Mechanical_wounding_promotes_local_and_long_distance_response_in_the_halophyte_Cakile_maritima_through_the_involvement_of_the_ROS_and_RNS_metabolism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1089-8603(17)30086-1 DB - PRIME DP - Unbound Medicine ER -