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Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor.
Anal Chem. 2017 08 01; 89(15):8050-8056.AC

Abstract

We report a multicolor ECL device based on closed bipolar electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)3]2+ and [Ir(ppy)3] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green-yellow-red ECL emission changing. As the first multicolor ECL device in biological analysis, BPE may raise the application of potential-resolved ECL to a new level.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang YZ
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Xu CH
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Zhao W
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Guan QY
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Chen HY
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Xu JJ
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.

MeSH

Biosensing TechniquesCoordination ComplexesElectrochemical TechniquesElectrodesHumansLuminescent MeasurementsOrganometallic CompoundsPropylaminesProstate-Specific Antigen

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28660761

Citation

Wang, Yin-Zhu, et al. "Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor." Analytical Chemistry, vol. 89, no. 15, 2017, pp. 8050-8056.
Wang YZ, Xu CH, Zhao W, et al. Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor. Anal Chem. 2017;89(15):8050-8056.
Wang, Y. Z., Xu, C. H., Zhao, W., Guan, Q. Y., Chen, H. Y., & Xu, J. J. (2017). Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor. Analytical Chemistry, 89(15), 8050-8056. https://doi.org/10.1021/acs.analchem.7b01494
Wang YZ, et al. Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor. Anal Chem. 2017 08 1;89(15):8050-8056. PubMed PMID: 28660761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor. AU - Wang,Yin-Zhu, AU - Xu,Cong-Hui, AU - Zhao,Wei, AU - Guan,Qi-Yuan, AU - Chen,Hong-Yuan, AU - Xu,Jing-Juan, Y1 - 2017/07/10/ PY - 2017/7/1/pubmed PY - 2019/2/15/medline PY - 2017/6/30/entrez SP - 8050 EP - 8056 JF - Analytical chemistry JO - Anal Chem VL - 89 IS - 15 N2 - We report a multicolor ECL device based on closed bipolar electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)3]2+ and [Ir(ppy)3] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green-yellow-red ECL emission changing. As the first multicolor ECL device in biological analysis, BPE may raise the application of potential-resolved ECL to a new level. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/28660761/Bipolar_Electrode_Based_Multicolor_Electrochemiluminescence_Biosensor_ L2 - https://doi.org/10.1021/acs.analchem.7b01494 DB - PRIME DP - Unbound Medicine ER -