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Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets.
Int J Mol Sci. 2017 Jun 29; 18(7)IJ

Abstract

The differential impact of ageing and cytomegalovirus (CMV) latent infection on human T-cell subsets remains to some extent controversial. The purpose of this study was to analyse the expression of the transcription factors T-bet and Eomes and CD57 on CD4+, CD4hiCD8lo and CD8+ T-cell subsets in healthy individuals, stratified by age and CMV serostatus. The percentage of CD4+ T-cells expressing T-bet or Eomes was very low, in particular in CD4+ T-cells from young CMV-seronegative individuals, and were higher in CMV-seropositive older individuals, in both CD57- and CD57+ CD4+ T-cells. The study of the minor peripheral blood double-positive CD4hiCD8lo T-cells showed that the percentage of these T-cells expressing both Eomes and T-bet was higher compared to CD4+ T-cells. The percentage of CD4hiCD8lo T-cells expressing T-bet was also associated with CMV seropositivity and the coexpression of Eomes, T-bet and CD57 on CD4hiCD8lo T-cells was only observed in CMV-seropositive donors, supporting the hypothesis that these cells are mature effector memory cells. The percentage of T-cells expressing Eomes and T-bet was higher in CD8+ T-cells than in CD4+ T-cells. The percentages of CD8+ T-cells expressing Eomes and T-bet increased with age in CMV-seronegative and -seropositive individuals and the percentages of CD57- CD8+ and CD57+ CD8+ T-cells coexpressing both transcription factors were similar in the different groups studied. These results support that CMV chronic infection and/or ageing are associated to the expansion of highly differentiated CD4+, CD4hiCD8lo and CD8+ T-cells that differentially express T-bet and Eomes suggesting that the expression of these transcription factors is essential for the generation and development of an effector-memory and effector T lymphocytes involved in conferring protection against chronic CMV infection.

Authors+Show Affiliations

Immunology Unit, IMIBIC-Hospital Universitario Reina Sofia-Universidad de Cordoba, Córdoba 14004, Spain. hassounehfakhri@yahoo.com.Immunology Unit, IMIBIC-Hospital Universitario Reina Sofia-Universidad de Cordoba, Córdoba 14004, Spain. nelsonj836@hotmail.com.Immunology Unit, IMIBIC-Hospital Universitario Reina Sofia-Universidad de Cordoba, Córdoba 14004, Spain. mccampos1977@gmail.com.Immunology Unit, Department of Physiology, University of Extremadura, Cáceres 10003, Spain. beatrizsanchezcorrea@gmail.com.Immunology Unit, Department of Physiology, University of Extremadura, Cáceres 10003, Spain. rtarazon@unex.es.Immunology Unit, IMIBIC-Hospital Universitario Reina Sofia-Universidad de Cordoba, Córdoba 14004, Spain. alejandrapera@gmail.com. Division of Clinical and Experimental Medicine, Brighton and Sussex Medical School, Brighton BN1 9PS, UK. alejandrapera@gmail.com.Immunology Unit, IMIBIC-Hospital Universitario Reina Sofia-Universidad de Cordoba, Córdoba 14004, Spain. rsolana@unex.es. Immunology Unit, Department of Physiology, University of Extremadura, Cáceres 10003, Spain. rsolana@unex.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28661443

Citation

Hassouneh, Fakhri, et al. "Effect of Cytomegalovirus (CMV) and Ageing On T-Bet and Eomes Expression On T-Cell Subsets." International Journal of Molecular Sciences, vol. 18, no. 7, 2017.
Hassouneh F, Lopez-Sejas N, Campos C, et al. Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets. Int J Mol Sci. 2017;18(7).
Hassouneh, F., Lopez-Sejas, N., Campos, C., Sanchez-Correa, B., Tarazona, R., Pera, A., & Solana, R. (2017). Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets. International Journal of Molecular Sciences, 18(7). https://doi.org/10.3390/ijms18071391
Hassouneh F, et al. Effect of Cytomegalovirus (CMV) and Ageing On T-Bet and Eomes Expression On T-Cell Subsets. Int J Mol Sci. 2017 Jun 29;18(7) PubMed PMID: 28661443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets. AU - Hassouneh,Fakhri, AU - Lopez-Sejas,Nelson, AU - Campos,Carmen, AU - Sanchez-Correa,Beatriz, AU - Tarazona,Raquel, AU - Pera,Alejandra, AU - Solana,Rafael, Y1 - 2017/06/29/ PY - 2017/05/11/received PY - 2017/06/15/revised PY - 2017/06/26/accepted PY - 2017/6/30/entrez PY - 2017/7/1/pubmed PY - 2018/3/24/medline KW - CD57 KW - Eomes KW - T-bet KW - T-cells KW - ageing KW - cytomegalovirus (CMV) JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 7 N2 - The differential impact of ageing and cytomegalovirus (CMV) latent infection on human T-cell subsets remains to some extent controversial. The purpose of this study was to analyse the expression of the transcription factors T-bet and Eomes and CD57 on CD4+, CD4hiCD8lo and CD8+ T-cell subsets in healthy individuals, stratified by age and CMV serostatus. The percentage of CD4+ T-cells expressing T-bet or Eomes was very low, in particular in CD4+ T-cells from young CMV-seronegative individuals, and were higher in CMV-seropositive older individuals, in both CD57- and CD57+ CD4+ T-cells. The study of the minor peripheral blood double-positive CD4hiCD8lo T-cells showed that the percentage of these T-cells expressing both Eomes and T-bet was higher compared to CD4+ T-cells. The percentage of CD4hiCD8lo T-cells expressing T-bet was also associated with CMV seropositivity and the coexpression of Eomes, T-bet and CD57 on CD4hiCD8lo T-cells was only observed in CMV-seropositive donors, supporting the hypothesis that these cells are mature effector memory cells. The percentage of T-cells expressing Eomes and T-bet was higher in CD8+ T-cells than in CD4+ T-cells. The percentages of CD8+ T-cells expressing Eomes and T-bet increased with age in CMV-seronegative and -seropositive individuals and the percentages of CD57- CD8+ and CD57+ CD8+ T-cells coexpressing both transcription factors were similar in the different groups studied. These results support that CMV chronic infection and/or ageing are associated to the expansion of highly differentiated CD4+, CD4hiCD8lo and CD8+ T-cells that differentially express T-bet and Eomes suggesting that the expression of these transcription factors is essential for the generation and development of an effector-memory and effector T lymphocytes involved in conferring protection against chronic CMV infection. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28661443/Effect_of_Cytomegalovirus__CMV__and_Ageing_on_T_Bet_and_Eomes_Expression_on_T_Cell_Subsets_ L2 - https://www.mdpi.com/resolver?pii=ijms18071391 DB - PRIME DP - Unbound Medicine ER -