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Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye.
J Biomed Opt 2017; 22(6):60504JB

Abstract

Visualization and assessment of the cellular structure and function require localized delivery of the molecules into specific cells in restricted spatial regions of the tissue and may necessitate subcellular delivery and localization. Earlier, we have shown ultrafast near-infrared laser beam-assisted optoporation of actin-staining molecules into cortical neurons with single-cell resolution and high efficiency. However, diffusion of optoporated molecules in soma degrades toward the growth cone, leading to difficulties in visualization of the actin network in the growth cone in cases of long axons. Here, we demonstrate optoporation of impermeable molecules to functional cortical neurons by precise laser subaxotomy near the growth cone, leading to visualization of the actin network in the growth cone. Further, we demonstrate patterned delivery of impermeable molecules into targeted retinal cells in the rat eye. The development of optoporation as a minimally invasive approach to reliably deliver exogenous molecules into targeted axons and soma of retinal neurons in vivo will enable enhanced visualization of the structure and function of the retina.

Authors+Show Affiliations

NanoScope Technologies LLC, Bedford, Texas, United States.University of Texas at Arlington, Department of Bioengineering, Texas, United States.NanoScope Technologies LLC, Bedford, Texas, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28662241

Citation

Batabyal, Subrata, et al. "Ultrafast Laser-assisted Spatially Targeted Optoporation Into Cortical Axons and Retinal Cells in the Eye." Journal of Biomedical Optics, vol. 22, no. 6, 2017, p. 60504.
Batabyal S, Kim YT, Mohanty S. Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye. J Biomed Opt. 2017;22(6):60504.
Batabyal, S., Kim, Y. T., & Mohanty, S. (2017). Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye. Journal of Biomedical Optics, 22(6), p. 60504. doi:10.1117/1.JBO.22.6.060504.
Batabyal S, Kim YT, Mohanty S. Ultrafast Laser-assisted Spatially Targeted Optoporation Into Cortical Axons and Retinal Cells in the Eye. J Biomed Opt. 2017 06 1;22(6):60504. PubMed PMID: 28662241.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrafast laser-assisted spatially targeted optoporation into cortical axons and retinal cells in the eye. AU - Batabyal,Subrata, AU - Kim,Young-Tae, AU - Mohanty,Samarendra, PY - 2017/04/27/received PY - 2017/06/13/accepted PY - 2017/6/30/entrez PY - 2017/7/1/pubmed PY - 2018/7/19/medline SP - 60504 EP - 60504 JF - Journal of biomedical optics JO - J Biomed Opt VL - 22 IS - 6 N2 - Visualization and assessment of the cellular structure and function require localized delivery of the molecules into specific cells in restricted spatial regions of the tissue and may necessitate subcellular delivery and localization. Earlier, we have shown ultrafast near-infrared laser beam-assisted optoporation of actin-staining molecules into cortical neurons with single-cell resolution and high efficiency. However, diffusion of optoporated molecules in soma degrades toward the growth cone, leading to difficulties in visualization of the actin network in the growth cone in cases of long axons. Here, we demonstrate optoporation of impermeable molecules to functional cortical neurons by precise laser subaxotomy near the growth cone, leading to visualization of the actin network in the growth cone. Further, we demonstrate patterned delivery of impermeable molecules into targeted retinal cells in the rat eye. The development of optoporation as a minimally invasive approach to reliably deliver exogenous molecules into targeted axons and soma of retinal neurons in vivo will enable enhanced visualization of the structure and function of the retina. SN - 1560-2281 UR - https://www.unboundmedicine.com/medline/citation/28662241/Ultrafast_laser_assisted_spatially_targeted_optoporation_into_cortical_axons_and_retinal_cells_in_the_eye_ L2 - https://doi.org/10.1117/1.JBO.22.6.060504 DB - PRIME DP - Unbound Medicine ER -