Tags

Type your tag names separated by a space and hit enter

Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats.
J Biochem Mol Toxicol. 2017 Oct; 31(10)JB

Abstract

In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2',5'-ADP-Sepharose 4B affinity gel. The effects of Ast and Al3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al3+ inhibited the enzyme activity noncompetitively (IC50 values; 0.679 mM, Ki values 1.32 mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al + Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P < 0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al + Ast groups (P < 0.05).

Authors+Show Affiliations

Program of Medical Laboratory Techniques, Department of Medical Services and Techniques, Solhan Vocational School of Health Services, Bingöl University, Bingöl, Turkey.Program of Medical Laboratory Techniques, Department of Medical Services and Techniques, Vocational School of Health Services, Bingöl University, Bingöl, Turkey.Faculty of Veterinary, Siirt Universty, Siirt, Turkey.Faculty of Veterinary, Siirt Universty, Siirt, Turkey.Department of Chemistry, Faculty of Arts and Sciences, Bingöl University, Bingöl, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28665548

Citation

Temel, Yusuf, et al. "Effect of Astaxanthin and Aluminum Chloride On Erythrocyte G6PD and 6PGD Enzyme Activities in Vivo and On Erythrocyte G6PD in Vitro in Rats." Journal of Biochemical and Molecular Toxicology, vol. 31, no. 10, 2017.
Temel Y, Bengü AŞ, Akkoyun HT, et al. Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats. J Biochem Mol Toxicol. 2017;31(10).
Temel, Y., Bengü, A. Ş., Akkoyun, H. T., Akkoyun, M., & Ciftci, M. (2017). Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats. Journal of Biochemical and Molecular Toxicology, 31(10). https://doi.org/10.1002/jbt.21954
Temel Y, et al. Effect of Astaxanthin and Aluminum Chloride On Erythrocyte G6PD and 6PGD Enzyme Activities in Vivo and On Erythrocyte G6PD in Vitro in Rats. J Biochem Mol Toxicol. 2017;31(10) PubMed PMID: 28665548.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats. AU - Temel,Yusuf, AU - Bengü,Aydın Şükrü, AU - Akkoyun,Hurrem Turan, AU - Akkoyun,Mahire, AU - Ciftci,Mehmet, Y1 - 2017/06/30/ PY - 2017/04/10/received PY - 2017/05/30/revised PY - 2017/06/16/accepted PY - 2017/7/1/pubmed PY - 2018/1/3/medline PY - 2017/7/1/entrez KW - aluminum KW - astaxanthin KW - enzyme purification KW - glucose-6-phosphate dehydrogenase (G6PD); 6-phosphogluconate dehydrogenase (6PGD) KW - inhibition KW - rat erythrocyte JF - Journal of biochemical and molecular toxicology JO - J Biochem Mol Toxicol VL - 31 IS - 10 N2 - In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2',5'-ADP-Sepharose 4B affinity gel. The effects of Ast and Al3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al3+ inhibited the enzyme activity noncompetitively (IC50 values; 0.679 mM, Ki values 1.32 mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al + Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group (P < 0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al + Ast groups (P < 0.05). SN - 1099-0461 UR - https://www.unboundmedicine.com/medline/citation/28665548/Effect_of_astaxanthin_and_aluminum_chloride_on_erythrocyte_G6PD_and_6PGD_enzyme_activities_in_vivo_and_on_erythrocyte_G6PD_in_vitro_in_rats_ L2 - https://doi.org/10.1002/jbt.21954 DB - PRIME DP - Unbound Medicine ER -