Tags

Type your tag names separated by a space and hit enter

Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS.
J Pharm Biomed Anal. 2017 Oct 25; 145:178-185.JP

Abstract

A novel gradient reverse phase high performance liquid chromatography tandem mass spectrometry (HPLC/MS-MS) was performed as a method for the determination of dobutamine hydrochloride (DOB) in newborn pig plasma samples. It was developed and validated after optimization of sample treatment and various chromatographic and mass spectrometric conditions. Trimethoxydobutamine (TMD) was used as internal standard. Heptafluorobutyric acid (HFBA) and ethyl acetate were used for the treatment of plasma samples. The separation of dobutamine and internal standard was done using a Kinetex F5 (50×2.1mm, 2.6μm, 100Å) analytical column. The mobile phase was a mixture of acetonitrile and HCOOH 0.01%. The column oven temperature was optimized at 40° C and the flow rate was 0.25mL/min. DOB and TMD were detected by multiple reaction monitoring (MRM) mode in ESI+, using a cone voltage (CV) of 25V and a collision energy (CE) of 25eV. The weighted calibration curve (1/x2) was found to be linear over the concentration range of 1-100ng/mL (r2>0.999). The limit of quantification (LLOQ) of the method was 1ng/mL. The values of selectivity, carryover, LLOQ, linearity, accuracy, precision, matrix effect, stability and recovery obtained meet the acceptable range according to European Medicines Agency (EMA) and Food and Drug Administration (FDA) guidelines. The method was efficiently applied to quantify DOB in plasma samples from a pharmacokinetic/pharmacodynamic study in a disease model of newborn piglet.

Authors+Show Affiliations

Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain.Dynakin S.L., Suite 801-B, Derio, Spain.Dynakin S.L., Suite 801-B, Derio, Spain.Analytical Chemistry Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), P.O. Box 644, 48080 Bilbao, Spain. Electronic address: rosamaria.alonso@ehu.eus.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28666164

Citation

Albóniga, O E., et al. "Quantitative Determination of Dobutamine in Newborn Pig Plasma Samples By HPLC-MS/MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 145, 2017, pp. 178-185.
Albóniga OE, Alonso ML, Blanco ME, et al. Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS. J Pharm Biomed Anal. 2017;145:178-185.
Albóniga, O. E., Alonso, M. L., Blanco, M. E., González, O., Grisaleña, A., Campanero, M. A., & Alonso, R. M. (2017). Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 145, 178-185. https://doi.org/10.1016/j.jpba.2017.06.050
Albóniga OE, et al. Quantitative Determination of Dobutamine in Newborn Pig Plasma Samples By HPLC-MS/MS. J Pharm Biomed Anal. 2017 Oct 25;145:178-185. PubMed PMID: 28666164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination of dobutamine in newborn pig plasma samples by HPLC-MS/MS. AU - Albóniga,O E, AU - Alonso,M L, AU - Blanco,M E, AU - González,O, AU - Grisaleña,A, AU - Campanero,M A, AU - Alonso,R M, Y1 - 2017/06/21/ PY - 2017/04/19/received PY - 2017/06/16/revised PY - 2017/06/20/accepted PY - 2017/7/1/pubmed PY - 2018/2/2/medline PY - 2017/7/1/entrez KW - Dobutamine KW - HPLC–MS/MS KW - Newborn pig KW - Plasma SP - 178 EP - 185 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 145 N2 - A novel gradient reverse phase high performance liquid chromatography tandem mass spectrometry (HPLC/MS-MS) was performed as a method for the determination of dobutamine hydrochloride (DOB) in newborn pig plasma samples. It was developed and validated after optimization of sample treatment and various chromatographic and mass spectrometric conditions. Trimethoxydobutamine (TMD) was used as internal standard. Heptafluorobutyric acid (HFBA) and ethyl acetate were used for the treatment of plasma samples. The separation of dobutamine and internal standard was done using a Kinetex F5 (50×2.1mm, 2.6μm, 100Å) analytical column. The mobile phase was a mixture of acetonitrile and HCOOH 0.01%. The column oven temperature was optimized at 40° C and the flow rate was 0.25mL/min. DOB and TMD were detected by multiple reaction monitoring (MRM) mode in ESI+, using a cone voltage (CV) of 25V and a collision energy (CE) of 25eV. The weighted calibration curve (1/x2) was found to be linear over the concentration range of 1-100ng/mL (r2>0.999). The limit of quantification (LLOQ) of the method was 1ng/mL. The values of selectivity, carryover, LLOQ, linearity, accuracy, precision, matrix effect, stability and recovery obtained meet the acceptable range according to European Medicines Agency (EMA) and Food and Drug Administration (FDA) guidelines. The method was efficiently applied to quantify DOB in plasma samples from a pharmacokinetic/pharmacodynamic study in a disease model of newborn piglet. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/28666164/Quantitative_determination_of_dobutamine_in_newborn_pig_plasma_samples_by_HPLC_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(17)30937-8 DB - PRIME DP - Unbound Medicine ER -