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The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast.
J Biol Chem. 2017 08 18; 292(33):13823-13832.JB

Abstract

Glycolysis and the pentose phosphate pathway both play a central role in the degradation of glucose in all domains of life. Another metabolic route that can facilitate glucose breakdown is the gluconate shunt. In this shunt glucose dehydrogenase and gluconate kinase catalyze the two-step conversion of glucose into the pentose phosphate pathway intermediate 6-phosphogluconate. Despite the presence of these enzymes in many organisms, their only established role is in the production of 6-phosphogluconate for the Entner-Doudoroff pathway. In this report we performed metabolic profiling on a strain of Schizosaccharomyces pombe lacking the zinc-responsive transcriptional repressor Loz1 with the goal of identifying metabolic pathways that were altered by cellular zinc status. This profiling revealed that loz1Δ cells accumulate higher levels of gluconate. We show that the altered gluconate levels in loz1Δ cells result from increased expression of gcd1 By analyzing the activity of recombinant Gcd1 in vitro and by measuring gluconate levels in strains lacking enzymes of the gluconate shunt we demonstrate that Gcd1 encodes a novel NADP+-dependent glucose dehydrogenase that acts in a pathway with the Idn1 gluconate kinase. We also find that cells lacking gcd1 and zwf1, which encode the first enzyme in the pentose phosphate pathway, have a more severe growth phenotype than cells lacking zwf1 We propose that in S. pombe Gcd1 and Idn1 act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase.

Authors+Show Affiliations

From the Department of Molecular Genetics.From the Department of Molecular Genetics.From the Department of Molecular Genetics. Center for Applied Plant Sciences.From the Department of Molecular Genetics. Center for Applied Plant Sciences.From the Department of Molecular Genetics, bird.96@osu.edu. Department of Human Nutrition, and. the Center for RNA Biology, The Ohio State University, Columbus, Ohio 43210.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28667014

Citation

Corkins, Mark E., et al. "The Gluconate Shunt Is an Alternative Route for Directing Glucose Into the Pentose Phosphate Pathway in Fission Yeast." The Journal of Biological Chemistry, vol. 292, no. 33, 2017, pp. 13823-13832.
Corkins ME, Wilson S, Cocuron JC, et al. The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast. J Biol Chem. 2017;292(33):13823-13832.
Corkins, M. E., Wilson, S., Cocuron, J. C., Alonso, A. P., & Bird, A. J. (2017). The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast. The Journal of Biological Chemistry, 292(33), 13823-13832. https://doi.org/10.1074/jbc.M117.798488
Corkins ME, et al. The Gluconate Shunt Is an Alternative Route for Directing Glucose Into the Pentose Phosphate Pathway in Fission Yeast. J Biol Chem. 2017 08 18;292(33):13823-13832. PubMed PMID: 28667014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The gluconate shunt is an alternative route for directing glucose into the pentose phosphate pathway in fission yeast. AU - Corkins,Mark E, AU - Wilson,Stevin, AU - Cocuron,Jean-Christophe, AU - Alonso,Ana P, AU - Bird,Amanda J, Y1 - 2017/06/30/ PY - 2017/05/23/received PY - 2017/06/26/revised PY - 2017/7/2/pubmed PY - 2017/9/7/medline PY - 2017/7/2/entrez KW - NADPH regeneration KW - gene expression KW - gluconate KW - glucose degradation KW - glucose metabolism KW - pentose phosphate pathway (PPP) KW - secondary metabolism KW - zinc SP - 13823 EP - 13832 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 292 IS - 33 N2 - Glycolysis and the pentose phosphate pathway both play a central role in the degradation of glucose in all domains of life. Another metabolic route that can facilitate glucose breakdown is the gluconate shunt. In this shunt glucose dehydrogenase and gluconate kinase catalyze the two-step conversion of glucose into the pentose phosphate pathway intermediate 6-phosphogluconate. Despite the presence of these enzymes in many organisms, their only established role is in the production of 6-phosphogluconate for the Entner-Doudoroff pathway. In this report we performed metabolic profiling on a strain of Schizosaccharomyces pombe lacking the zinc-responsive transcriptional repressor Loz1 with the goal of identifying metabolic pathways that were altered by cellular zinc status. This profiling revealed that loz1Δ cells accumulate higher levels of gluconate. We show that the altered gluconate levels in loz1Δ cells result from increased expression of gcd1 By analyzing the activity of recombinant Gcd1 in vitro and by measuring gluconate levels in strains lacking enzymes of the gluconate shunt we demonstrate that Gcd1 encodes a novel NADP+-dependent glucose dehydrogenase that acts in a pathway with the Idn1 gluconate kinase. We also find that cells lacking gcd1 and zwf1, which encode the first enzyme in the pentose phosphate pathway, have a more severe growth phenotype than cells lacking zwf1 We propose that in S. pombe Gcd1 and Idn1 act together to shunt glucose into the pentose phosphate pathway, creating an alternative route for directing glucose into the pentose phosphate pathway that bypasses hexokinase and the rate-limiting enzyme glucose-6-phosphate dehydrogenase. SN - 1083-351X UR - https://www.unboundmedicine.com/medline/citation/28667014/The_gluconate_shunt_is_an_alternative_route_for_directing_glucose_into_the_pentose_phosphate_pathway_in_fission_yeast_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=28667014 DB - PRIME DP - Unbound Medicine ER -