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Lifespan-extending and stress resistance properties of brazilin from Caesalpinia sappan in Caenorhabditis elegans.
Arch Pharm Res 2017; 40(7):825-835AP

Abstract

This study contributes to the continual discovery of lifespan-extending compounds from plants, using the Caenorhabditis elegans model system. An ethyl acetate soluble fraction of methanol extract from the heartwood of Caesalpinia sappan showed a significant lifespan-extending activity. Subsequent activity-guided chromatography of the ethyl acetate-soluble fraction led to the isolation of brazilin. Brazilin showed potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide anion quenching activities and also revealed a lifespan-extending activity in C. elegans under normal culture conditions. Brazilin also exhibited the protective effects against thermal, oxidative and osmotic stress conditions to improve the survival rate of the nematode. Furthermore, brazilin elevated superoxide dismutase (SOD) activity and decreased intracellular reactive oxygen species accumulation in C. elegans. Further studies showed that brazilin-mediated increased stress tolerance of worms could be due to increased expressions of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). Besides, there were no significant, brazilin-induced changes in aging-related factors, including progeny production, food intake, and growth, indicating brazilin influences longevity activity independent of affecting these factors. Brazilin increased the body movement of aged worms, indicating brazilin affects the healthspan and lifespan of nematode. These results suggest that brazilin contributes to the lifespan of C. elegans under both normal and stress conditions by increasing the expressions of stress resistance proteins.

Authors+Show Affiliations

College of Pharmacy, Woosuk University, Jeonju, 55338, Republic of Korea.College of Pharmacy, Woosuk University, Jeonju, 55338, Republic of Korea. College of Materials and Chemistry Engineering, Tongren University, Guizhou, 554300, China.College of Pharmacy, Woosuk University, Jeonju, 55338, Republic of Korea. dkkim@woosuk.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28667441

Citation

Lee, Eun Byeol, et al. "Lifespan-extending and Stress Resistance Properties of Brazilin From Caesalpinia Sappan in Caenorhabditis Elegans." Archives of Pharmacal Research, vol. 40, no. 7, 2017, pp. 825-835.
Lee EB, Xing MM, Kim DK. Lifespan-extending and stress resistance properties of brazilin from Caesalpinia sappan in Caenorhabditis elegans. Arch Pharm Res. 2017;40(7):825-835.
Lee, E. B., Xing, M. M., & Kim, D. K. (2017). Lifespan-extending and stress resistance properties of brazilin from Caesalpinia sappan in Caenorhabditis elegans. Archives of Pharmacal Research, 40(7), pp. 825-835. doi:10.1007/s12272-017-0920-3.
Lee EB, Xing MM, Kim DK. Lifespan-extending and Stress Resistance Properties of Brazilin From Caesalpinia Sappan in Caenorhabditis Elegans. Arch Pharm Res. 2017;40(7):825-835. PubMed PMID: 28667441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lifespan-extending and stress resistance properties of brazilin from Caesalpinia sappan in Caenorhabditis elegans. AU - Lee,Eun Byeol, AU - Xing,Ming Ming, AU - Kim,Dae Keun, Y1 - 2017/06/30/ PY - 2016/08/23/received PY - 2017/05/08/accepted PY - 2017/7/2/pubmed PY - 2018/4/21/medline PY - 2017/7/2/entrez KW - Brazilin KW - Caenorhabditis elegans KW - Caesalpinia sappan KW - Lifespan-extending KW - Stress resistance SP - 825 EP - 835 JF - Archives of pharmacal research JO - Arch. Pharm. Res. VL - 40 IS - 7 N2 - This study contributes to the continual discovery of lifespan-extending compounds from plants, using the Caenorhabditis elegans model system. An ethyl acetate soluble fraction of methanol extract from the heartwood of Caesalpinia sappan showed a significant lifespan-extending activity. Subsequent activity-guided chromatography of the ethyl acetate-soluble fraction led to the isolation of brazilin. Brazilin showed potent 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide anion quenching activities and also revealed a lifespan-extending activity in C. elegans under normal culture conditions. Brazilin also exhibited the protective effects against thermal, oxidative and osmotic stress conditions to improve the survival rate of the nematode. Furthermore, brazilin elevated superoxide dismutase (SOD) activity and decreased intracellular reactive oxygen species accumulation in C. elegans. Further studies showed that brazilin-mediated increased stress tolerance of worms could be due to increased expressions of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). Besides, there were no significant, brazilin-induced changes in aging-related factors, including progeny production, food intake, and growth, indicating brazilin influences longevity activity independent of affecting these factors. Brazilin increased the body movement of aged worms, indicating brazilin affects the healthspan and lifespan of nematode. These results suggest that brazilin contributes to the lifespan of C. elegans under both normal and stress conditions by increasing the expressions of stress resistance proteins. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/28667441/Lifespan_extending_and_stress_resistance_properties_of_brazilin_from_Caesalpinia_sappan_in_Caenorhabditis_elegans_ L2 - https://dx.doi.org/10.1007/s12272-017-0920-3 DB - PRIME DP - Unbound Medicine ER -