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Human umbilical cord-derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction.
Nephrology (Carlton). 2018 Aug; 23(8):728-736.N

Abstract

AIM

The growing number of patients suffering from chronic renal disease (CKD) is a challenge for the development of innovative therapies. Researchers have studied the therapeutic effects of cell therapy in acute kidney injury (AKI). However, the therapeutic effect of conditional medium (CM) in the CKD models have been rarely reported. Here, we examined the effects of umbilical cord derived-mesenchymal stem cells (hUC-MSCs) CM on renal fibrosis in a rat model of unilateral ureteral obstruction (UUO).

METHODS

Animals were randomly divided into three groups: sham-operated, UUO, UUO + CM. CM was administered via the left renal artery after total ligation of the left ureter. Rats were killed after 14 days of obstruction. Histological changes and oxidative stress parameters were assessed. Western blotting and immunohistochemistry analysis were used to measure epithelial-mesenchymal transition (EMT) markers, including epithelial cadherin (E-cadherin), α-smooth muscle actin (α-SMA), tumour necrosis factor-α (TNF-α), Collagen-I, and transforming growth factor β1 (TGF-β1). Proliferation and apoptosis of renal tubular epithelial cells (RTEs) were also measured.

RESULTS

HucMSC-CM significantly reduced the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), and increased the activity of glutathione (GSH) induced by UUO. Moreover, CM significantly reduced the expression of TGF-β1, α-SMA, TNF-α and Collagen-I in UUO kidney, promoted the proliferation of RTEs and inhibited its apoptosis. In addition, the increased expression of E-cadherin also reflects the effective improvement of renal interstitial fibrosis.

CONCLUSION

This study shows that CM protects UUO-induced kidney damage and therefore could be a potential tool to prevent CKD progression.

Authors+Show Affiliations

Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China.Department of Respiratory Medicine, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China.Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China. Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing, China.Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28667820

Citation

Liu, Bo, et al. "Human Umbilical Cord-derived Mesenchymal Stem Cells Conditioned Medium Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells in an Irreversible Model of Unilateral Ureteral Obstruction." Nephrology (Carlton, Vic.), vol. 23, no. 8, 2018, pp. 728-736.
Liu B, Ding FX, Liu Y, et al. Human umbilical cord-derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction. Nephrology (Carlton). 2018;23(8):728-736.
Liu, B., Ding, F. X., Liu, Y., Xiong, G., Lin, T., He, D. W., Zhang, Y. Y., Zhang, D. Y., & Wei, G. H. (2018). Human umbilical cord-derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction. Nephrology (Carlton, Vic.), 23(8), 728-736. https://doi.org/10.1111/nep.13099
Liu B, et al. Human Umbilical Cord-derived Mesenchymal Stem Cells Conditioned Medium Attenuate Interstitial Fibrosis and Stimulate the Repair of Tubular Epithelial Cells in an Irreversible Model of Unilateral Ureteral Obstruction. Nephrology (Carlton). 2018;23(8):728-736. PubMed PMID: 28667820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human umbilical cord-derived mesenchymal stem cells conditioned medium attenuate interstitial fibrosis and stimulate the repair of tubular epithelial cells in an irreversible model of unilateral ureteral obstruction. AU - Liu,Bo, AU - Ding,Feng-Xia, AU - Liu,Yang, AU - Xiong,Geng, AU - Lin,Tao, AU - He,Da-Wei, AU - Zhang,Yuan-Yuan, AU - Zhang,De-Ying, AU - Wei,Guang-Hui, PY - 2017/01/04/received PY - 2017/05/11/revised PY - 2017/06/25/accepted PY - 2017/7/2/pubmed PY - 2019/7/30/medline PY - 2017/7/2/entrez KW - Fibrosis KW - Mesenchymal stem cells KW - Repair KW - Unilateral ureteral obstruction SP - 728 EP - 736 JF - Nephrology (Carlton, Vic.) JO - Nephrology (Carlton) VL - 23 IS - 8 N2 - AIM: The growing number of patients suffering from chronic renal disease (CKD) is a challenge for the development of innovative therapies. Researchers have studied the therapeutic effects of cell therapy in acute kidney injury (AKI). However, the therapeutic effect of conditional medium (CM) in the CKD models have been rarely reported. Here, we examined the effects of umbilical cord derived-mesenchymal stem cells (hUC-MSCs) CM on renal fibrosis in a rat model of unilateral ureteral obstruction (UUO). METHODS: Animals were randomly divided into three groups: sham-operated, UUO, UUO + CM. CM was administered via the left renal artery after total ligation of the left ureter. Rats were killed after 14 days of obstruction. Histological changes and oxidative stress parameters were assessed. Western blotting and immunohistochemistry analysis were used to measure epithelial-mesenchymal transition (EMT) markers, including epithelial cadherin (E-cadherin), α-smooth muscle actin (α-SMA), tumour necrosis factor-α (TNF-α), Collagen-I, and transforming growth factor β1 (TGF-β1). Proliferation and apoptosis of renal tubular epithelial cells (RTEs) were also measured. RESULTS: HucMSC-CM significantly reduced the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), and increased the activity of glutathione (GSH) induced by UUO. Moreover, CM significantly reduced the expression of TGF-β1, α-SMA, TNF-α and Collagen-I in UUO kidney, promoted the proliferation of RTEs and inhibited its apoptosis. In addition, the increased expression of E-cadherin also reflects the effective improvement of renal interstitial fibrosis. CONCLUSION: This study shows that CM protects UUO-induced kidney damage and therefore could be a potential tool to prevent CKD progression. SN - 1440-1797 UR - https://www.unboundmedicine.com/medline/citation/28667820/Human_umbilical_cord_derived_mesenchymal_stem_cells_conditioned_medium_attenuate_interstitial_fibrosis_and_stimulate_the_repair_of_tubular_epithelial_cells_in_an_irreversible_model_of_unilateral_ureteral_obstruction_ L2 - https://doi.org/10.1111/nep.13099 DB - PRIME DP - Unbound Medicine ER -