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Silver nanoparticles from Pilimelia columellifera subsp. pallida SL19 strain demonstrated antifungal activity against fungi causing superficial mycoses.
J Basic Microbiol. 2017 Sep; 57(9):793-800.JB

Abstract

In this study, we present the in vitro antifungal activity of silver nanoparticles (AgNPs) synthesized from acidophilic actinobacterium Pilimelia columellifera subsp. pallida SL19 strain, alone and in combination with antibiotics viz., amphotericin B, fluconazole, and ketoconazole against pathogenic fungi, namely Candida albicans, Malassezia furfur, and Trichophyton erinacei. The minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNPs against test fungi were evaluated. The fractional inhibitory concentration (FIC) index was determined to estimate antifungal activity of AgNPs combined with antibiotics. Antifungal activity of AgNPs varied among the tested fungal strains. M. furfur was found to be most sensitive to biogenic silver nanoparticles, followed by C. albicans and T. erinacei. The lowest MIC of AgNPs was noticed against M. furfur (16 μg ml-1). Synergistic effect was observed on C. albicans when AgNP were combined with amphotericin B and ketoconazole and on M. furfur with fluconazole and ketoconazole (FIC index of 0.5). Cytotoxic effect of AgNPs on HeLa and 3T3 cell lines was evaluated. The IC50 values were found to be 55 and 25 μg ml-1 , respectively. The present study indicates that silver nanoparticles from P. columellifera subsp. pallida SL19 strain have antifungal activity, both alone and in combination with antibiotics, and offer a valuable contribution to nanomedicine.

Authors+Show Affiliations

Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland.Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.Nanobiotechnology Lab., Department of Biotechnology, SGB Amravati University, Amravati, Maharashtra, India.Department of Microbiology, Nicolaus Copernicus University, Torun, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28670763

Citation

Wypij, Magdalena, et al. "Silver Nanoparticles From Pilimelia Columellifera Subsp. Pallida SL19 Strain Demonstrated Antifungal Activity Against Fungi Causing Superficial Mycoses." Journal of Basic Microbiology, vol. 57, no. 9, 2017, pp. 793-800.
Wypij M, Czarnecka J, Dahm H, et al. Silver nanoparticles from Pilimelia columellifera subsp. pallida SL19 strain demonstrated antifungal activity against fungi causing superficial mycoses. J Basic Microbiol. 2017;57(9):793-800.
Wypij, M., Czarnecka, J., Dahm, H., Rai, M., & Golinska, P. (2017). Silver nanoparticles from Pilimelia columellifera subsp. pallida SL19 strain demonstrated antifungal activity against fungi causing superficial mycoses. Journal of Basic Microbiology, 57(9), 793-800. https://doi.org/10.1002/jobm.201700121
Wypij M, et al. Silver Nanoparticles From Pilimelia Columellifera Subsp. Pallida SL19 Strain Demonstrated Antifungal Activity Against Fungi Causing Superficial Mycoses. J Basic Microbiol. 2017;57(9):793-800. PubMed PMID: 28670763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles from Pilimelia columellifera subsp. pallida SL19 strain demonstrated antifungal activity against fungi causing superficial mycoses. AU - Wypij,Magdalena, AU - Czarnecka,Joanna, AU - Dahm,Hanna, AU - Rai,Mahendra, AU - Golinska,Patrycja, Y1 - 2017/07/03/ PY - 2017/02/23/received PY - 2017/05/30/revised PY - 2017/06/06/accepted PY - 2017/7/4/pubmed PY - 2018/4/24/medline PY - 2017/7/4/entrez KW - antifungal activity KW - biogenic silver nanoparticles KW - dermatophytes KW - fungal pathogens SP - 793 EP - 800 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 57 IS - 9 N2 - In this study, we present the in vitro antifungal activity of silver nanoparticles (AgNPs) synthesized from acidophilic actinobacterium Pilimelia columellifera subsp. pallida SL19 strain, alone and in combination with antibiotics viz., amphotericin B, fluconazole, and ketoconazole against pathogenic fungi, namely Candida albicans, Malassezia furfur, and Trichophyton erinacei. The minimum inhibitory concentration (MIC) and minimum biocidal concentration (MBC) of AgNPs against test fungi were evaluated. The fractional inhibitory concentration (FIC) index was determined to estimate antifungal activity of AgNPs combined with antibiotics. Antifungal activity of AgNPs varied among the tested fungal strains. M. furfur was found to be most sensitive to biogenic silver nanoparticles, followed by C. albicans and T. erinacei. The lowest MIC of AgNPs was noticed against M. furfur (16 μg ml-1). Synergistic effect was observed on C. albicans when AgNP were combined with amphotericin B and ketoconazole and on M. furfur with fluconazole and ketoconazole (FIC index of 0.5). Cytotoxic effect of AgNPs on HeLa and 3T3 cell lines was evaluated. The IC50 values were found to be 55 and 25 μg ml-1 , respectively. The present study indicates that silver nanoparticles from P. columellifera subsp. pallida SL19 strain have antifungal activity, both alone and in combination with antibiotics, and offer a valuable contribution to nanomedicine. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/28670763/Silver_nanoparticles_from_Pilimelia_columellifera_subsp__pallida_SL19_strain_demonstrated_antifungal_activity_against_fungi_causing_superficial_mycoses_ DB - PRIME DP - Unbound Medicine ER -