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FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals.
Int J Mol Sci 2017; 18(7)IJ

Abstract

Photoconvertible fluorescent proteins (pcRFPs) are a group of fluorophores that undergo an irreversible green-to-red shift in emission colour upon irradiation with near-ultraviolet (near-UV) light. Despite their wide application in biotechnology, the high-level expression of pcRFPs in mesophotic and depth-generalist coral species currently lacks a biological explanation. Additionally, reduced penetration of near-UV wavelengths in water poses the question whether light-driven photoconversion is relevant in the mesophotic zone, or whether a different mechanism is involved in the post-translational pigment modification in vivo. Here, we show in a long-term mesocosm experiment that photoconversion in vivo is entirely dependent on near-UV wavelengths. However, a near-UV intensity equivalent to the mesophotic underwater light field at 80 m depth is sufficient to drive the process in vitro, suggesting that photoconversion can occur near the lower distribution limits of these corals. Furthermore, live coral colonies showed evidence of efficient Förster Resonance Energy Transfer (FRET). Our simulated mesophotic light field maintained the pcRFP pool in a partially photoconverted state in vivo, maximising intra-tetrameric FRET and creating a long-range wavelength conversion system with higher quantum yield than other native RFPs. We hypothesise that efficient conversion of blue wavelengths, abundant at depth, into orange-red light could constitute an adaptation of corals to life in light-limited environments.

Authors+Show Affiliations

Coral Reef Laboratory, University of Southampton, Waterfront Campus, European Way, Southampton SO143ZH, UK. e.bollati@soton.ac.uk.Coral Reef Laboratory, University of Southampton, Waterfront Campus, European Way, Southampton SO143ZH, UK. dp5g11@southamptonalumni.ac.uk.Coral Reef Laboratory, University of Southampton, Waterfront Campus, European Way, Southampton SO143ZH, UK. C.D'angelo@soton.ac.uk. Institute for Life Sciences (IFLS), University of Southampton, Highfield Campus, Southampton SO171BJ, UK. C.D'angelo@soton.ac.uk.Coral Reef Laboratory, University of Southampton, Waterfront Campus, European Way, Southampton SO143ZH, UK. joerg.wiedenmann@noc.soton.ac.uk. Institute for Life Sciences (IFLS), University of Southampton, Highfield Campus, Southampton SO171BJ, UK. joerg.wiedenmann@noc.soton.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28677653

Citation

Bollati, Elena, et al. "FRET-Mediated Long-Range Wavelength Transformation By Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals." International Journal of Molecular Sciences, vol. 18, no. 7, 2017.
Bollati E, Plimmer D, D'Angelo C, et al. FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals. Int J Mol Sci. 2017;18(7).
Bollati, E., Plimmer, D., D'Angelo, C., & Wiedenmann, J. (2017). FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals. International Journal of Molecular Sciences, 18(7), doi:10.3390/ijms18071174.
Bollati E, et al. FRET-Mediated Long-Range Wavelength Transformation By Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals. Int J Mol Sci. 2017 Jul 4;18(7) PubMed PMID: 28677653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals. AU - Bollati,Elena, AU - Plimmer,Daniel, AU - D'Angelo,Cecilia, AU - Wiedenmann,Jörg, Y1 - 2017/07/04/ PY - 2017/04/07/received PY - 2017/05/15/revised PY - 2017/05/17/accepted PY - 2017/7/6/entrez PY - 2017/7/6/pubmed PY - 2018/3/27/medline KW - FRET KW - coral KW - fluorescence KW - fluorescent protein KW - mesophotic KW - photoconversion JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 7 N2 - Photoconvertible fluorescent proteins (pcRFPs) are a group of fluorophores that undergo an irreversible green-to-red shift in emission colour upon irradiation with near-ultraviolet (near-UV) light. Despite their wide application in biotechnology, the high-level expression of pcRFPs in mesophotic and depth-generalist coral species currently lacks a biological explanation. Additionally, reduced penetration of near-UV wavelengths in water poses the question whether light-driven photoconversion is relevant in the mesophotic zone, or whether a different mechanism is involved in the post-translational pigment modification in vivo. Here, we show in a long-term mesocosm experiment that photoconversion in vivo is entirely dependent on near-UV wavelengths. However, a near-UV intensity equivalent to the mesophotic underwater light field at 80 m depth is sufficient to drive the process in vitro, suggesting that photoconversion can occur near the lower distribution limits of these corals. Furthermore, live coral colonies showed evidence of efficient Förster Resonance Energy Transfer (FRET). Our simulated mesophotic light field maintained the pcRFP pool in a partially photoconverted state in vivo, maximising intra-tetrameric FRET and creating a long-range wavelength conversion system with higher quantum yield than other native RFPs. We hypothesise that efficient conversion of blue wavelengths, abundant at depth, into orange-red light could constitute an adaptation of corals to life in light-limited environments. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28677653/FRET_Mediated_Long_Range_Wavelength_Transformation_by_Photoconvertible_Fluorescent_Proteins_as_an_Efficient_Mechanism_to_Generate_Orange_Red_Light_in_Symbiotic_Deep_Water_Corals_ L2 - http://www.mdpi.com/resolver?pii=ijms18071174 DB - PRIME DP - Unbound Medicine ER -