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Characterization and discrimination of raw and vinegar-baked Bupleuri radix based on UHPLC-Q-TOF-MS coupled with multivariate statistical analysis.
Biomed Chromatogr 2018; 32(2)BC

Abstract

Bupleuri Radix is a commonly used herb in clinic, and raw and vinegar-baked Bupleuri Radix are both documented in the Pharmacopoeia of People's Republic of China. According to the theories of traditional Chinese medicine, Bupleuri Radix possesses different therapeutic effects before and after processing. However, the chemical mechanism of this processing is still unknown. In this study, ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry coupled with multivariate statistical analysis including principal component analysis and orthogonal partial least square-discriminant analysis was developed to holistically compare the difference between raw and vinegar-baked Bupleuri Radix for the first time. As a result, 50 peaks in raw and processed Bupleuri Radix were detected, respectively, and a total of 49 peak chemical compounds were identified. Saikosaponin a, saikosaponin d, saikosaponin b3 , saikosaponin e, saikosaponin c, saikosaponin b2 , saikosaponin b1 , 4''-O-acetyl-saikosaponin d, hyperoside and 3',4'-dimethoxy quercetin were explored as potential markers of raw and vinegar-baked Bupleuri Radix. This study has been successfully applied for global analysis of raw and vinegar-processed samples. Furthermore, the underlying hepatoprotective mechanism of Bupleuri Radix was predicted, which was related to the changes of chemical profiling.

Authors+Show Affiliations

Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Pharmaceutical Botany, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.Department of Medicinal Plant, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28677911

Citation

Lei, Tianli, et al. "Characterization and Discrimination of Raw and Vinegar-baked Bupleuri Radix Based On UHPLC-Q-TOF-MS Coupled With Multivariate Statistical Analysis." Biomedical Chromatography : BMC, vol. 32, no. 2, 2018.
Lei T, Chen S, Wang K, et al. Characterization and discrimination of raw and vinegar-baked Bupleuri radix based on UHPLC-Q-TOF-MS coupled with multivariate statistical analysis. Biomed Chromatogr. 2018;32(2).
Lei, T., Chen, S., Wang, K., Zhang, D., Dong, L., Lv, C., ... Lu, J. (2018). Characterization and discrimination of raw and vinegar-baked Bupleuri radix based on UHPLC-Q-TOF-MS coupled with multivariate statistical analysis. Biomedical Chromatography : BMC, 32(2), doi:10.1002/bmc.4044.
Lei T, et al. Characterization and Discrimination of Raw and Vinegar-baked Bupleuri Radix Based On UHPLC-Q-TOF-MS Coupled With Multivariate Statistical Analysis. Biomed Chromatogr. 2018;32(2) PubMed PMID: 28677911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and discrimination of raw and vinegar-baked Bupleuri radix based on UHPLC-Q-TOF-MS coupled with multivariate statistical analysis. AU - Lei,Tianli, AU - Chen,Shifeng, AU - Wang,Kai, AU - Zhang,Dandan, AU - Dong,Lin, AU - Lv,Chongning, AU - Wang,Jing, AU - Lu,Jincai, Y1 - 2017/07/28/ PY - 2017/03/07/received PY - 2017/06/19/revised PY - 2017/06/28/accepted PY - 2017/7/6/pubmed PY - 2018/2/27/medline PY - 2017/7/6/entrez KW - Bupleuri radix KW - UHPLC-q-TOF-MS KW - chemical marker KW - multivariate statistical analysis KW - processing mechanism JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 32 IS - 2 N2 - Bupleuri Radix is a commonly used herb in clinic, and raw and vinegar-baked Bupleuri Radix are both documented in the Pharmacopoeia of People's Republic of China. According to the theories of traditional Chinese medicine, Bupleuri Radix possesses different therapeutic effects before and after processing. However, the chemical mechanism of this processing is still unknown. In this study, ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry coupled with multivariate statistical analysis including principal component analysis and orthogonal partial least square-discriminant analysis was developed to holistically compare the difference between raw and vinegar-baked Bupleuri Radix for the first time. As a result, 50 peaks in raw and processed Bupleuri Radix were detected, respectively, and a total of 49 peak chemical compounds were identified. Saikosaponin a, saikosaponin d, saikosaponin b3 , saikosaponin e, saikosaponin c, saikosaponin b2 , saikosaponin b1 , 4''-O-acetyl-saikosaponin d, hyperoside and 3',4'-dimethoxy quercetin were explored as potential markers of raw and vinegar-baked Bupleuri Radix. This study has been successfully applied for global analysis of raw and vinegar-processed samples. Furthermore, the underlying hepatoprotective mechanism of Bupleuri Radix was predicted, which was related to the changes of chemical profiling. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/28677911/Characterization_and_discrimination_of_raw_and_vinegar_baked_Bupleuri_radix_based_on_UHPLC_Q_TOF_MS_coupled_with_multivariate_statistical_analysis_ L2 - https://doi.org/10.1002/bmc.4044 DB - PRIME DP - Unbound Medicine ER -