Tags

Type your tag names separated by a space and hit enter

Reduced interstitial cells of Cajal and increased intraepithelial lymphocytes are associated with development of small intestinal bacterial overgrowth in post-infectious IBS mouse model.
Scand J Gastroenterol. 2017 Oct; 52(10):1065-1071.SJ

Abstract

OBJECTIVE

Intestinal dysmotility and immune activation are likely involved in the pathogenesis of small intestinal bacteria overgrowth (SIBO) in irritable bowel syndrome (IBS). We aimed at investigating the role of interstitial cells of Cajal (ICC) and intestinal inflammation in the development of SIBO using a post-infectious IBS (PI-IBS) mouse model.

MATERIALS AND METHODS

NIH mice were randomly infected with Trichinella spiralis. Visceral sensitivity and stool pattern were assessed at 8-weeks post-infection (PI). Intestinal bacteria counts from jejunum and ileum were measured by quantitative real-time PCR to evaluate the presence of SIBO. ICC density, intraepithelial lymphocytes (IELs) counts, and intestinal cytokine levels (IL1-β, IL-6, toll-like receptor-4 (TLR-4), IL-10) in the ileum were examined.

RESULTS

PI-IBS mice demonstrated increased visceral sensitivity compared with the control group. One-third of the PI-IBS mice developed SIBO (SIBO+/PI-IBS) and was more likely to have abnormal stool form compared with SIBO negative PI-IBS (SIBO-/PI-IBS) mice but without difference in visceral sensitivity. SIBO+/PI-IBS mice had decreased ICC density and increased IELs counts in the ileum compared with SIBO-/PI-IBS mice. No difference in inflammatory cytokine expression levels were detected among the groups except for increased TLR-4 in PI-IBS mice compared with the control group.

CONCLUSIONS

Development of SIBO in PI-IBS mice was associated with reduced ICC density and increased IELs counts in the ileum. Our findings support the role of intestinal dysmotility and inflammation in the pathogenesis of SIBO in IBS and may provide potential therapeutic targets.

Links

Publisher Full Text

Authors+Show Affiliations

Chen B
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.
Zhu S
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China. b Department of Gastroenterology , Zhejiang Cancer Hospital , Hangzhou , Zhejiang , China.
Du L
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.
He H
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.
Kim JJ
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China. c Division of Gastroenterology , Loma Linda University Medical Center , Loma Linda , USA.
Dai N
a Department of Gastroenterology , Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University , Hangzhou , Zhejiang , China.

MeSH

AnimalsBlind Loop SyndromeDisease Models, AnimalIleumInterleukin-10Interleukin-1betaInterleukin-6Interstitial Cells of CajalIntestinal MucosaIrritable Bowel SyndromeJejunumLymphocyte CountMaleMiceToll-Like Receptor 4Trichinella spiralisTrichinellosis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28679338

Citation

Chen, Binrui, et al. "Reduced Interstitial Cells of Cajal and Increased Intraepithelial Lymphocytes Are Associated With Development of Small Intestinal Bacterial Overgrowth in Post-infectious IBS Mouse Model." Scandinavian Journal of Gastroenterology, vol. 52, no. 10, 2017, pp. 1065-1071.
Chen B, Zhu S, Du L, et al. Reduced interstitial cells of Cajal and increased intraepithelial lymphocytes are associated with development of small intestinal bacterial overgrowth in post-infectious IBS mouse model. Scand J Gastroenterol. 2017;52(10):1065-1071.
Chen, B., Zhu, S., Du, L., He, H., Kim, J. J., & Dai, N. (2017). Reduced interstitial cells of Cajal and increased intraepithelial lymphocytes are associated with development of small intestinal bacterial overgrowth in post-infectious IBS mouse model. Scandinavian Journal of Gastroenterology, 52(10), 1065-1071. https://doi.org/10.1080/00365521.2017.1342141
Chen B, et al. Reduced Interstitial Cells of Cajal and Increased Intraepithelial Lymphocytes Are Associated With Development of Small Intestinal Bacterial Overgrowth in Post-infectious IBS Mouse Model. Scand J Gastroenterol. 2017;52(10):1065-1071. PubMed PMID: 28679338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced interstitial cells of Cajal and increased intraepithelial lymphocytes are associated with development of small intestinal bacterial overgrowth in post-infectious IBS mouse model. AU - Chen,Binrui, AU - Zhu,Shuwen, AU - Du,Lijun, AU - He,Huiqin, AU - Kim,John J, AU - Dai,Ning, Y1 - 2017/07/05/ PY - 2017/7/7/pubmed PY - 2018/7/14/medline PY - 2017/7/7/entrez KW - Small intestinal bacterial overgrowth KW - interstitial cells of Cajal KW - intraepithelial lymphocyte KW - irritable bowel syndrome SP - 1065 EP - 1071 JF - Scandinavian journal of gastroenterology JO - Scand J Gastroenterol VL - 52 IS - 10 N2 - OBJECTIVE: Intestinal dysmotility and immune activation are likely involved in the pathogenesis of small intestinal bacteria overgrowth (SIBO) in irritable bowel syndrome (IBS). We aimed at investigating the role of interstitial cells of Cajal (ICC) and intestinal inflammation in the development of SIBO using a post-infectious IBS (PI-IBS) mouse model. MATERIALS AND METHODS: NIH mice were randomly infected with Trichinella spiralis. Visceral sensitivity and stool pattern were assessed at 8-weeks post-infection (PI). Intestinal bacteria counts from jejunum and ileum were measured by quantitative real-time PCR to evaluate the presence of SIBO. ICC density, intraepithelial lymphocytes (IELs) counts, and intestinal cytokine levels (IL1-β, IL-6, toll-like receptor-4 (TLR-4), IL-10) in the ileum were examined. RESULTS: PI-IBS mice demonstrated increased visceral sensitivity compared with the control group. One-third of the PI-IBS mice developed SIBO (SIBO+/PI-IBS) and was more likely to have abnormal stool form compared with SIBO negative PI-IBS (SIBO-/PI-IBS) mice but without difference in visceral sensitivity. SIBO+/PI-IBS mice had decreased ICC density and increased IELs counts in the ileum compared with SIBO-/PI-IBS mice. No difference in inflammatory cytokine expression levels were detected among the groups except for increased TLR-4 in PI-IBS mice compared with the control group. CONCLUSIONS: Development of SIBO in PI-IBS mice was associated with reduced ICC density and increased IELs counts in the ileum. Our findings support the role of intestinal dysmotility and inflammation in the pathogenesis of SIBO in IBS and may provide potential therapeutic targets. SN - 1502-7708 UR - https://www.unboundmedicine.com/medline/citation/28679338/Reduced_interstitial_cells_of_Cajal_and_increased_intraepithelial_lymphocytes_are_associated_with_development_of_small_intestinal_bacterial_overgrowth_in_post_infectious_IBS_mouse_model_ L2 - https://www.tandfonline.com/doi/full/10.1080/00365521.2017.1342141 DB - PRIME DP - Unbound Medicine ER -