Tags

Type your tag names separated by a space and hit enter

Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China.
Sci Rep. 2017 07 05; 7(1):4706.SR

Abstract

In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045, SE33, D10S1248), two Y-chromosome markers (DYS391 and Y-indel) and the sex determining marker, Amelogenin was developed with six fluorescent dyes labeling. The included STR loci belonged to the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS) as well as some additional loci commonly used in commercial kits and national DNA databases. This paper describes the validation studies conducted with the SureID PanGlobal system using Applied Biosystems 3500 XL Genetic Analyzer for fragment length detection that included the analysis of the following parameters and aspects: PCR conditions, sensitivity, species specificity, inhibition, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated that the SureID PanGlobal system is reproducible, accurate, sensitive and robust for forensic application and databasing. Additionally, the whole cycling time of the system can finish within 65 minutes, which was developed specifically for rapid and reliable generation of DNA profiles obtained from blood, buccal swabs and forensic stains.

Authors+Show Affiliations

Institute of Criminal Sciences and Technology, Municipal Public Security Bureau of Xuchang, Xuchang, 461000, P.R. China.Henan Provincal Public Security Department, Criminal Science and Technology Institute, Zhengzhou, 450003, P.R. China.Health GeneTech, Ningbo, 315000, P.R. China.Health GeneTech, Ningbo, 315000, P.R. China.Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, Beijing, 100088, P.R. China. Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou, 515041, P.R. China.Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, Beijing, 100088, P.R. China. shimeisen2000@163.com. Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou, 515041, P.R. China. shimeisen2000@163.com.Department of Radiology, First Affiliated Hospital, Medical College of Shantou University, Shantou, 515041, P.R. China. mashuhua6699@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28680088

Citation

Liu, Yaju, et al. "Developmental Validation of a 6-dye Typing System With 27 Loci and Application in Han Population of China." Scientific Reports, vol. 7, no. 1, 2017, p. 4706.
Liu Y, Guo L, Jin H, et al. Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China. Sci Rep. 2017;7(1):4706.
Liu, Y., Guo, L., Jin, H., Li, Z., Bai, R., Shi, M., & Ma, S. (2017). Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China. Scientific Reports, 7(1), 4706. https://doi.org/10.1038/s41598-017-04548-1
Liu Y, et al. Developmental Validation of a 6-dye Typing System With 27 Loci and Application in Han Population of China. Sci Rep. 2017 07 5;7(1):4706. PubMed PMID: 28680088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China. AU - Liu,Yaju, AU - Guo,Lihong, AU - Jin,Haiying, AU - Li,Zheng, AU - Bai,Rufeng, AU - Shi,Meisen, AU - Ma,Shuhua, Y1 - 2017/07/05/ PY - 2016/05/27/received PY - 2017/05/17/accepted PY - 2017/7/7/entrez PY - 2017/7/7/pubmed PY - 2019/1/30/medline SP - 4706 EP - 4706 JF - Scientific reports JO - Sci Rep VL - 7 IS - 1 N2 - In this study, a novel 27-locus system (now known as the SureID PanGlobal system), including 24 autosomal STRs (D3S1358, TH01, D21S11, D18S51, Penta E, D12S391, D6S1043, D2S1338, D1S1656, D2S441, D5S818, D13S317, D7S820, D19S433, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA, D16S539, D22S1045, SE33, D10S1248), two Y-chromosome markers (DYS391 and Y-indel) and the sex determining marker, Amelogenin was developed with six fluorescent dyes labeling. The included STR loci belonged to the core loci in the Combined DNA Index System (CODIS) and the European Standard Set (ESS) as well as some additional loci commonly used in commercial kits and national DNA databases. This paper describes the validation studies conducted with the SureID PanGlobal system using Applied Biosystems 3500 XL Genetic Analyzer for fragment length detection that included the analysis of the following parameters and aspects: PCR conditions, sensitivity, species specificity, inhibition, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated that the SureID PanGlobal system is reproducible, accurate, sensitive and robust for forensic application and databasing. Additionally, the whole cycling time of the system can finish within 65 minutes, which was developed specifically for rapid and reliable generation of DNA profiles obtained from blood, buccal swabs and forensic stains. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28680088/Developmental_validation_of_a_6_dye_typing_system_with_27_loci_and_application_in_Han_population_of_China_ L2 - https://doi.org/10.1038/s41598-017-04548-1 DB - PRIME DP - Unbound Medicine ER -