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Gene expression profiles and molecular mechanism of cultured human chondrocytes' exposure to T-2 toxin and deoxynivalenol.
Toxicon. 2017 Dec 15; 140:38-44.T

Abstract

T-2 toxin and deoxynivalenol (DON) are secondary metabolites produced by Fusarium fungi and are commonly found on food and feed. Although T-2 toxin and DON have been suggested as the etiology of Kashin-Beck disease (KBD), an endemic osteochondropathy, little is known about the mechanism when human chondrocytes are exposed to T-2 toxin and DON. The purpose of this study is to identify the gene expression differences and underlying molecular changes modulated by T-2 toxin and DON in vitro in human chondrocytes. After the experiments of cell viability, the gene expression profiles were analyzed in cells that were treated with 0.01 μg/ml T-2 toxin and 1.0 μg/ml DON for 72 h by Affymetrix Human Gene Chip. The array results showed that 882 and 2118 genes were differentially expressed for T-2 toxin and DON exposure, respectively. Enrichment analysis revealed that diverse cellular processes including DNA damage, cell cycle regulation and metabolism of extracellular matrix were affected when human chondrocytes were exposed to T-2 toxin and DON. These results demonstrate the gene expression differences and molecular mechanism of cultured human chondrocytes exposure to T-2 toxin and DON, and provide a new insight into future research in the etiology of KBD.

Authors+Show Affiliations

School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China.School of Clinical Medicine, Hainan Medical University, Haikou 571199, Hainan, PR China.Hong Hui Hospital, Health Science Center, Xi'an Jiaotong University, Xi'an 710054, Shaanxi, PR China.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China. Electronic address: wn18andlife@xjtu.edu.cn.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China; Department of Integrative Medical Biology, University of Umeå, Umeå, Sweden.School of Public Health, Health Science Center, Xi'an Jiaotong University, Key Laboratory of Trace Elements and Endemic Diseases of National Health and Family Planning Commission, Xi'an 710061, Shaanxi, PR China. Electronic address: guox@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28684119

Citation

Yang, Lei, et al. "Gene Expression Profiles and Molecular Mechanism of Cultured Human Chondrocytes' Exposure to T-2 Toxin and Deoxynivalenol." Toxicon : Official Journal of the International Society On Toxinology, vol. 140, 2017, pp. 38-44.
Yang L, Zhang J, Zhao G, et al. Gene expression profiles and molecular mechanism of cultured human chondrocytes' exposure to T-2 toxin and deoxynivalenol. Toxicon. 2017;140:38-44.
Yang, L., Zhang, J., Zhao, G., Wu, C., Ning, Y., Wang, X., Lammi, M. J., & Guo, X. (2017). Gene expression profiles and molecular mechanism of cultured human chondrocytes' exposure to T-2 toxin and deoxynivalenol. Toxicon : Official Journal of the International Society On Toxinology, 140, 38-44. https://doi.org/10.1016/j.toxicon.2017.06.014
Yang L, et al. Gene Expression Profiles and Molecular Mechanism of Cultured Human Chondrocytes' Exposure to T-2 Toxin and Deoxynivalenol. Toxicon. 2017 Dec 15;140:38-44. PubMed PMID: 28684119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression profiles and molecular mechanism of cultured human chondrocytes' exposure to T-2 toxin and deoxynivalenol. AU - Yang,Lei, AU - Zhang,Jianping, AU - Zhao,Guanghui, AU - Wu,Cuiyan, AU - Ning,Yujie, AU - Wang,Xi, AU - Lammi,Mikko J, AU - Guo,Xiong, Y1 - 2017/07/03/ PY - 2017/03/13/received PY - 2017/06/21/revised PY - 2017/06/28/accepted PY - 2017/7/8/pubmed PY - 2019/1/10/medline PY - 2017/7/8/entrez KW - Deoxynivalenol KW - Human chondrocytes KW - Microarray KW - T-2 toxin SP - 38 EP - 44 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 140 N2 - T-2 toxin and deoxynivalenol (DON) are secondary metabolites produced by Fusarium fungi and are commonly found on food and feed. Although T-2 toxin and DON have been suggested as the etiology of Kashin-Beck disease (KBD), an endemic osteochondropathy, little is known about the mechanism when human chondrocytes are exposed to T-2 toxin and DON. The purpose of this study is to identify the gene expression differences and underlying molecular changes modulated by T-2 toxin and DON in vitro in human chondrocytes. After the experiments of cell viability, the gene expression profiles were analyzed in cells that were treated with 0.01 μg/ml T-2 toxin and 1.0 μg/ml DON for 72 h by Affymetrix Human Gene Chip. The array results showed that 882 and 2118 genes were differentially expressed for T-2 toxin and DON exposure, respectively. Enrichment analysis revealed that diverse cellular processes including DNA damage, cell cycle regulation and metabolism of extracellular matrix were affected when human chondrocytes were exposed to T-2 toxin and DON. These results demonstrate the gene expression differences and molecular mechanism of cultured human chondrocytes exposure to T-2 toxin and DON, and provide a new insight into future research in the etiology of KBD. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/28684119/Gene_expression_profiles_and_molecular_mechanism_of_cultured_human_chondrocytes'_exposure_to_T_2_toxin_and_deoxynivalenol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(17)30203-9 DB - PRIME DP - Unbound Medicine ER -