Tags

Type your tag names separated by a space and hit enter

Whole transcriptome RNAseq analysis of Oenococcus oeni reveals distinct intra-specific expression patterns during malolactic fermentation, including genes involved in diacetyl metabolism.
Int J Food Microbiol. 2017 Sep 18; 257:216-224.IJ

Abstract

We report the first whole transcriptome RNAseq analysis of the wine-associated lactic acid bacterium Oenococcus oeni using a combination of reference-based mapping and de novo transcript assembly in three distinct strains during malolactic fermentation in Cabernet Sauvignon wine. Two of the strains (AWRIB551 and AWRIB552) exhibited similar transcriptomes relative to the third strain (AWRIB419) which was dissimilar by comparison. Significant intra-specific variation for genes related to glycolysis/gluconeogenesis, purine metabolism, aminoacyl-tRNA biosynthesis, ABC transporters and phosphotransferase systems was observed. Importantly, thirteen genes associated with the production of diacetyl, a commercially valuable aroma and flavour compound, were also found to be differentially expressed between the strains in a manner that correlated positively with total diacetyl production. This included a key strain-specific gene that is predicted to encode a l-lactate dehydrogenase that may enable l-lactic acid to be utilised as a precursor for the production of diacetyl. In conjunction with previous comparative genomic studies of O. oeni, this study progresses the understanding of genetic variations which contribute to the phenotypes of this industrially-important bacterium.

Authors+Show Affiliations

The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia 5064, Australia.The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia 5064, Australia.The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia 5064, Australia.The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia 5064, Australia.The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia 5064, Australia; Department of Genetics and Evolution, University of Adelaide, South Australia 5000, Australia. Electronic address: anthony.borneman@awri.com.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28688370

Citation

Sternes, Peter R., et al. "Whole Transcriptome RNAseq Analysis of Oenococcus Oeni Reveals Distinct Intra-specific Expression Patterns During Malolactic Fermentation, Including Genes Involved in Diacetyl Metabolism." International Journal of Food Microbiology, vol. 257, 2017, pp. 216-224.
Sternes PR, Costello PJ, Chambers PJ, et al. Whole transcriptome RNAseq analysis of Oenococcus oeni reveals distinct intra-specific expression patterns during malolactic fermentation, including genes involved in diacetyl metabolism. Int J Food Microbiol. 2017;257:216-224.
Sternes, P. R., Costello, P. J., Chambers, P. J., Bartowsky, E. J., & Borneman, A. R. (2017). Whole transcriptome RNAseq analysis of Oenococcus oeni reveals distinct intra-specific expression patterns during malolactic fermentation, including genes involved in diacetyl metabolism. International Journal of Food Microbiology, 257, 216-224. https://doi.org/10.1016/j.ijfoodmicro.2017.06.024
Sternes PR, et al. Whole Transcriptome RNAseq Analysis of Oenococcus Oeni Reveals Distinct Intra-specific Expression Patterns During Malolactic Fermentation, Including Genes Involved in Diacetyl Metabolism. Int J Food Microbiol. 2017 Sep 18;257:216-224. PubMed PMID: 28688370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Whole transcriptome RNAseq analysis of Oenococcus oeni reveals distinct intra-specific expression patterns during malolactic fermentation, including genes involved in diacetyl metabolism. AU - Sternes,Peter R, AU - Costello,Peter J, AU - Chambers,Paul J, AU - Bartowsky,Eveline J, AU - Borneman,Anthony R, Y1 - 2017/06/28/ PY - 2017/04/20/received PY - 2017/06/06/revised PY - 2017/06/25/accepted PY - 2017/7/9/pubmed PY - 2017/12/12/medline PY - 2017/7/9/entrez KW - Diacetyl KW - Gene expression KW - Industrial microbiology KW - Malolactic fermentation KW - Oenococcus oeni KW - RNA sequencing KW - Transcriptome KW - Wine SP - 216 EP - 224 JF - International journal of food microbiology JO - Int. J. Food Microbiol. VL - 257 N2 - We report the first whole transcriptome RNAseq analysis of the wine-associated lactic acid bacterium Oenococcus oeni using a combination of reference-based mapping and de novo transcript assembly in three distinct strains during malolactic fermentation in Cabernet Sauvignon wine. Two of the strains (AWRIB551 and AWRIB552) exhibited similar transcriptomes relative to the third strain (AWRIB419) which was dissimilar by comparison. Significant intra-specific variation for genes related to glycolysis/gluconeogenesis, purine metabolism, aminoacyl-tRNA biosynthesis, ABC transporters and phosphotransferase systems was observed. Importantly, thirteen genes associated with the production of diacetyl, a commercially valuable aroma and flavour compound, were also found to be differentially expressed between the strains in a manner that correlated positively with total diacetyl production. This included a key strain-specific gene that is predicted to encode a l-lactate dehydrogenase that may enable l-lactic acid to be utilised as a precursor for the production of diacetyl. In conjunction with previous comparative genomic studies of O. oeni, this study progresses the understanding of genetic variations which contribute to the phenotypes of this industrially-important bacterium. SN - 1879-3460 UR - https://www.unboundmedicine.com/medline/citation/28688370/Whole_transcriptome_RNAseq_analysis_of_Oenococcus_oeni_reveals_distinct_intra_specific_expression_patterns_during_malolactic_fermentation_including_genes_involved_in_diacetyl_metabolism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1605(17)30292-1 DB - PRIME DP - Unbound Medicine ER -