Tags

Type your tag names separated by a space and hit enter

Fusion of a superfamily 1 helicase and an inactivated DNA polymerase is a signature of common evolutionary history of Polintons, polinton-like viruses, Tlr1 transposons and transpovirons.
Virus Evol. 2016 Jan; 2(1):vew019.VE

Abstract

Polintons (polintoviruses), polinton-like viruses (PLVs) and virophages belong to a recently described major class of eukaryotic viruses that is characterized by a distinct virion morphogenetic protein module and, in many members, a protein-primed family B DNA polymerase (pDNAP). All Polintons, by definition, encode a pDNAP and a retrovirus-like integrase. Most of the PLV lack these genes and instead encode a large protein containing a superfamily 1 (SF1) helicase domain. We show here that the SF1 helicase domain-containing proteins of the PLV also contain an inactivated pDNAP domain. This unique helicase-pDNAP fusion is also encoded by transpovirons, enigmatic plasmid-like genetic elements that are associated with giant viruses of the family Mimiviridae. These findings indicate the directionality of evolution of different groups of viruses and mobile elements in the Polinton-centered class. We propose that the PLV evolved from a polinton via fusion of the pDNAP gene with a helicase gene that was accompanied by mutations in the pDNAP active site, likely resulting in inactivation of the polymerase activity. The transpovirons could have evolved from PLV via the loss of several genes including those encoding the morphogenetic module proteins. These findings reaffirm the central evolutionary position of the Polintons in the evolution of eukaryotic viruses and other mobile genetic elements.

Authors+Show Affiliations

Department of Microbiology, Institut Pasteur, Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Paris, France.National Library of Medicine, National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA.National Library of Medicine, National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28694999

Citation

Krupovic, Mart, et al. "Fusion of a Superfamily 1 Helicase and an Inactivated DNA Polymerase Is a Signature of Common Evolutionary History of Polintons, Polinton-like Viruses, Tlr1 Transposons and Transpovirons." Virus Evolution, vol. 2, no. 1, 2016, pp. vew019.
Krupovic M, Yutin N, Koonin EV. Fusion of a superfamily 1 helicase and an inactivated DNA polymerase is a signature of common evolutionary history of Polintons, polinton-like viruses, Tlr1 transposons and transpovirons. Virus Evol. 2016;2(1):vew019.
Krupovic, M., Yutin, N., & Koonin, E. V. (2016). Fusion of a superfamily 1 helicase and an inactivated DNA polymerase is a signature of common evolutionary history of Polintons, polinton-like viruses, Tlr1 transposons and transpovirons. Virus Evolution, 2(1), vew019. https://doi.org/10.1093/ve/vew019
Krupovic M, Yutin N, Koonin EV. Fusion of a Superfamily 1 Helicase and an Inactivated DNA Polymerase Is a Signature of Common Evolutionary History of Polintons, Polinton-like Viruses, Tlr1 Transposons and Transpovirons. Virus Evol. 2016;2(1):vew019. PubMed PMID: 28694999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fusion of a superfamily 1 helicase and an inactivated DNA polymerase is a signature of common evolutionary history of Polintons, polinton-like viruses, Tlr1 transposons and transpovirons. AU - Krupovic,Mart, AU - Yutin,Natalya, AU - Koonin,Eugene V, Y1 - 2016/06/12/ PY - 2017/7/12/entrez PY - 2017/7/12/pubmed PY - 2017/7/12/medline SP - vew019 EP - vew019 JF - Virus evolution JO - Virus Evol VL - 2 IS - 1 N2 - Polintons (polintoviruses), polinton-like viruses (PLVs) and virophages belong to a recently described major class of eukaryotic viruses that is characterized by a distinct virion morphogenetic protein module and, in many members, a protein-primed family B DNA polymerase (pDNAP). All Polintons, by definition, encode a pDNAP and a retrovirus-like integrase. Most of the PLV lack these genes and instead encode a large protein containing a superfamily 1 (SF1) helicase domain. We show here that the SF1 helicase domain-containing proteins of the PLV also contain an inactivated pDNAP domain. This unique helicase-pDNAP fusion is also encoded by transpovirons, enigmatic plasmid-like genetic elements that are associated with giant viruses of the family Mimiviridae. These findings indicate the directionality of evolution of different groups of viruses and mobile elements in the Polinton-centered class. We propose that the PLV evolved from a polinton via fusion of the pDNAP gene with a helicase gene that was accompanied by mutations in the pDNAP active site, likely resulting in inactivation of the polymerase activity. The transpovirons could have evolved from PLV via the loss of several genes including those encoding the morphogenetic module proteins. These findings reaffirm the central evolutionary position of the Polintons in the evolution of eukaryotic viruses and other mobile genetic elements. SN - 2057-1577 UR - https://www.unboundmedicine.com/medline/citation/28694999/Fusion_of_a_superfamily_1_helicase_and_an_inactivated_DNA_polymerase_is_a_signature_of_common_evolutionary_history_of_Polintons_polinton_like_viruses_Tlr1_transposons_and_transpovirons_ L2 - https://academic.oup.com/ve/article-lookup/doi/10.1093/ve/vew019 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.