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Uptake and toxicity of CuO nanoparticles to Daphnia magna varies between indirect dietary and direct waterborne exposures.
Aquat Toxicol. 2017 Sep; 190:78-86.AT

Abstract

Research examining the direct and indirect ecological effects of nanomaterials in aquatic ecosystems is important for developing a more realistic understanding of the environmental implications of nanotechnology. Copper oxide nanoparticles (CuO NPs) are being used extensively in many industries but are considered highly toxic to aquatic species residing in surface waters. Few studies have addressed whether CuO NPs can be transferred through the aquatic food chain, and if such indirect exposure to nanomaterials impacts their toxicity. We investigated the uptake and trophic transfer of CuO NPs from the algae Chlorella vulgaris to the crustacean Daphnia magna and assessed bio-partitioning and resulting toxicity. We hypothesized that CuO NPs can be associated with algal cells and be transported to predators through feeding, and that the chronic toxicity can be altered in comparison to direct CuO NP exposure. For the indirect feeding exposure, algae pre-incubated with CuO NPs (Cu-algae) were washed to remove loose NPs and fed to D. magna while Cu uptake and toxicity were evaluated. For the direct waterborne exposures, a parallel group of D. magna were exposed to equivalent concentrations of CuO NPs while being fed unexposed algae. Using hyperspectral imaging we observed strong surface associations between pre-incubated CuO NPs and algae used in the feeding exposure, and quantified the average Cu content (0.15mg Cu/L) with ICP-OES. Cu accumulated in daphnid bodies to a greater extent in direct exposures, whereas molted carapaces and neonate offspring had more copper following the indirect feeding exposure, implying that D. magna may regulate internal Cu differently depending on the method of CuO NP delivery. Significantly higher D. magna mortality was observed following direct exposure relative to feeding exposure, and neonate production from adult daphnids exposed indirectly to CuO NPs was significantly reduced. Thus, nanoparticle interaction with biota at one trophic level may alter the biological response at the next trophic level in a way that is dependent on the delivery scenario. This study highlights the importance of evaluating potential ecological impacts of nanomaterials in more relevant, complex exposure scenarios.

Authors+Show Affiliations

School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, United States. Electronic address: wufa@oregonstate.edu.Department of Integrative Biology, Oregon State University, Corvallis, OR, 97331, United States. Electronic address: bortveda@oregonstate.edu.Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331, United States. Electronic address: Bryan.Harper@oregonstate.edu.School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, United States. Electronic address: crandonl@oregonstate.edu.School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, United States; Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97331, United States; Oregon Nanoscience and Microtechnologies Institute, Eugene, OR, United States. Electronic address: Stacey.harper@oregonstate.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28697458

Citation

Wu, Fan, et al. "Uptake and Toxicity of CuO Nanoparticles to Daphnia Magna Varies Between Indirect Dietary and Direct Waterborne Exposures." Aquatic Toxicology (Amsterdam, Netherlands), vol. 190, 2017, pp. 78-86.
Wu F, Bortvedt A, Harper BJ, et al. Uptake and toxicity of CuO nanoparticles to Daphnia magna varies between indirect dietary and direct waterborne exposures. Aquat Toxicol. 2017;190:78-86.
Wu, F., Bortvedt, A., Harper, B. J., Crandon, L. E., & Harper, S. L. (2017). Uptake and toxicity of CuO nanoparticles to Daphnia magna varies between indirect dietary and direct waterborne exposures. Aquatic Toxicology (Amsterdam, Netherlands), 190, 78-86. https://doi.org/10.1016/j.aquatox.2017.06.021
Wu F, et al. Uptake and Toxicity of CuO Nanoparticles to Daphnia Magna Varies Between Indirect Dietary and Direct Waterborne Exposures. Aquat Toxicol. 2017;190:78-86. PubMed PMID: 28697458.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uptake and toxicity of CuO nanoparticles to Daphnia magna varies between indirect dietary and direct waterborne exposures. AU - Wu,Fan, AU - Bortvedt,Amy, AU - Harper,Bryan J, AU - Crandon,Lauren E, AU - Harper,Stacey L, Y1 - 2017/06/26/ PY - 2017/05/02/received PY - 2017/06/21/revised PY - 2017/06/23/accepted PY - 2017/7/12/pubmed PY - 2017/12/5/medline PY - 2017/7/12/entrez KW - Chronic toxicity KW - Copper oxide KW - Daphnia magna KW - Exposure route KW - Hyperspectral imaging KW - Trophic transfer SP - 78 EP - 86 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 190 N2 - Research examining the direct and indirect ecological effects of nanomaterials in aquatic ecosystems is important for developing a more realistic understanding of the environmental implications of nanotechnology. Copper oxide nanoparticles (CuO NPs) are being used extensively in many industries but are considered highly toxic to aquatic species residing in surface waters. Few studies have addressed whether CuO NPs can be transferred through the aquatic food chain, and if such indirect exposure to nanomaterials impacts their toxicity. We investigated the uptake and trophic transfer of CuO NPs from the algae Chlorella vulgaris to the crustacean Daphnia magna and assessed bio-partitioning and resulting toxicity. We hypothesized that CuO NPs can be associated with algal cells and be transported to predators through feeding, and that the chronic toxicity can be altered in comparison to direct CuO NP exposure. For the indirect feeding exposure, algae pre-incubated with CuO NPs (Cu-algae) were washed to remove loose NPs and fed to D. magna while Cu uptake and toxicity were evaluated. For the direct waterborne exposures, a parallel group of D. magna were exposed to equivalent concentrations of CuO NPs while being fed unexposed algae. Using hyperspectral imaging we observed strong surface associations between pre-incubated CuO NPs and algae used in the feeding exposure, and quantified the average Cu content (0.15mg Cu/L) with ICP-OES. Cu accumulated in daphnid bodies to a greater extent in direct exposures, whereas molted carapaces and neonate offspring had more copper following the indirect feeding exposure, implying that D. magna may regulate internal Cu differently depending on the method of CuO NP delivery. Significantly higher D. magna mortality was observed following direct exposure relative to feeding exposure, and neonate production from adult daphnids exposed indirectly to CuO NPs was significantly reduced. Thus, nanoparticle interaction with biota at one trophic level may alter the biological response at the next trophic level in a way that is dependent on the delivery scenario. This study highlights the importance of evaluating potential ecological impacts of nanomaterials in more relevant, complex exposure scenarios. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/28697458/Uptake_and_toxicity_of_CuO_nanoparticles_to_Daphnia_magna_varies_between_indirect_dietary_and_direct_waterborne_exposures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(17)30175-3 DB - PRIME DP - Unbound Medicine ER -