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Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var.
Fish Shellfish Immunol. 2017 Sep; 68:84-91.FS

Abstract

The aim of the present study was to investigate effects of dietary Lactobacillus delbrueckii (L. delbrueckii) on immune response, disease resistance against Aeromonas hydrophila (A. hydrophila), antioxidant capability and growth performance of Cyprinus carpio Huanghe var. 450 fish (mean weight of 1.05 ± 0.03 g) were randomly distributed into five groups that fed diets containing different levels of L. delbrueckii (0, 1 × 105, 1 × 106, 1 × 107 and 1 × 108 CFU g-1) for 8 weeks. The results showed that intestinal immune parameters such as lysozyme, acid phosphatase, and myeloperoxidase activities, immunoglobulin M content, and the survival rate were improved in fish fed with 1 × 106 and 1 × 107 CFU g-1L. delbrueckii. In addition, 1 × 107 CFU g-1L. delbrueckii supplementation down-regulated mRNA levels of TNF-α, IL-8, IL-1β and NF-κBp65, and up-regulated IL-10 and TGF-β mRNA levels in the intestine. The survival rate was significantly (P < 0.05) higher (68.33%) in fish fed 1 × 106 CFU g-1L. delbrueckii than the control diet-fed group (40%) after challenge by A. hydrophila. Fish fed with diet containing 1 × 106 CFU g-1L. delbrueckii showed higher antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and total antioxidant capacity (T-AOC) and lower MDA concentrations than those of the control group (P < 0.05). The relative gene expression (SOD, CAT, GPX) showed the same trend with their activities. In addition, the growth performance was significantly improved in fish fed with the diet containing 1 × 106 and 1 × 107 CFU g-1L. delbrueckii (P < 0.05). These results demonstrated that dietary optimal levels of L. delbrueckii enhanced immunity, disease resistance against A. hydrophila antioxidant capability and growth performance in Cyprinus carpio Huanghe var.

Authors+Show Affiliations

College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China. Electronic address: zhangchunnuan12@163.com.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China. Electronic address: jiliang_zhang@126.com.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28698125

Citation

Zhang, Chun-Nuan, et al. "Effects of Lactobacillus Delbrueckii On Immune Response, Disease Resistance Against Aeromonas Hydrophila, Antioxidant Capability and Growth Performance of Cyprinus Carpio Huanghe Var." Fish & Shellfish Immunology, vol. 68, 2017, pp. 84-91.
Zhang CN, Zhang JL, Guan WC, et al. Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var. Fish Shellfish Immunol. 2017;68:84-91.
Zhang, C. N., Zhang, J. L., Guan, W. C., Zhang, X. F., Guan, S. H., Zeng, Q. H., Cheng, G. F., & Cui, W. (2017). Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var. Fish & Shellfish Immunology, 68, 84-91. https://doi.org/10.1016/j.fsi.2017.07.012
Zhang CN, et al. Effects of Lactobacillus Delbrueckii On Immune Response, Disease Resistance Against Aeromonas Hydrophila, Antioxidant Capability and Growth Performance of Cyprinus Carpio Huanghe Var. Fish Shellfish Immunol. 2017;68:84-91. PubMed PMID: 28698125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var. AU - Zhang,Chun-Nuan, AU - Zhang,Ji-Liang, AU - Guan,Wen-Chao, AU - Zhang,Xiao-Fei, AU - Guan,Su-Hua, AU - Zeng,Qing-Hui, AU - Cheng,Gao-Feng, AU - Cui,Wei, Y1 - 2017/07/08/ PY - 2017/03/13/received PY - 2017/07/03/revised PY - 2017/07/07/accepted PY - 2017/7/13/pubmed PY - 2018/5/23/medline PY - 2017/7/13/entrez KW - Antioxidant KW - Cyprinus carpio Huanghe var KW - Disease resistance KW - Immune response KW - Lactobacillus delbrueckii SP - 84 EP - 91 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 68 N2 - The aim of the present study was to investigate effects of dietary Lactobacillus delbrueckii (L. delbrueckii) on immune response, disease resistance against Aeromonas hydrophila (A. hydrophila), antioxidant capability and growth performance of Cyprinus carpio Huanghe var. 450 fish (mean weight of 1.05 ± 0.03 g) were randomly distributed into five groups that fed diets containing different levels of L. delbrueckii (0, 1 × 105, 1 × 106, 1 × 107 and 1 × 108 CFU g-1) for 8 weeks. The results showed that intestinal immune parameters such as lysozyme, acid phosphatase, and myeloperoxidase activities, immunoglobulin M content, and the survival rate were improved in fish fed with 1 × 106 and 1 × 107 CFU g-1L. delbrueckii. In addition, 1 × 107 CFU g-1L. delbrueckii supplementation down-regulated mRNA levels of TNF-α, IL-8, IL-1β and NF-κBp65, and up-regulated IL-10 and TGF-β mRNA levels in the intestine. The survival rate was significantly (P < 0.05) higher (68.33%) in fish fed 1 × 106 CFU g-1L. delbrueckii than the control diet-fed group (40%) after challenge by A. hydrophila. Fish fed with diet containing 1 × 106 CFU g-1L. delbrueckii showed higher antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and total antioxidant capacity (T-AOC) and lower MDA concentrations than those of the control group (P < 0.05). The relative gene expression (SOD, CAT, GPX) showed the same trend with their activities. In addition, the growth performance was significantly improved in fish fed with the diet containing 1 × 106 and 1 × 107 CFU g-1L. delbrueckii (P < 0.05). These results demonstrated that dietary optimal levels of L. delbrueckii enhanced immunity, disease resistance against A. hydrophila antioxidant capability and growth performance in Cyprinus carpio Huanghe var. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/28698125/Effects_of_Lactobacillus_delbrueckii_on_immune_response_disease_resistance_against_Aeromonas_hydrophila_antioxidant_capability_and_growth_performance_of_Cyprinus_carpio_Huanghe_var_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(17)30405-9 DB - PRIME DP - Unbound Medicine ER -