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Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition.
Lipids Health Dis. 2017 Jul 12; 16(1):138.LH

Abstract

BACKGROUND

Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples. However, these associations require further confirmation in the Tunisian olive oil cultivars, and little is known about the effect of polymorphisms in fatty acid-related genes on olive oil mono- and poly- unsaturated fatty acids distribution.

METHODS

A set of olive oils from 12 Tunisian cultivars was chosen. The fatty acid composition of each olive oil sample was determined by gas chromatography. Statistical and modeling Bayesian analyses were used to assess whether the FAD2.1 and FAD2.3 genotypes were associated with fatty acids composition.

RESULTS

The TT-FAD2.1 and the GG-FAD2.3 genotypes were found to be associated with a lower proportion of oleic acid (C18:1) (r = -0.778, p = 0.003; r = -0.781, p= 0.003) as well as higher proportion of linoleic (C18:2) (r = 0.693, p = 0.012; r = -0.759, p= 0.004) and palmitic acids (C16:0) (r = 0.643, p = 0.024; r = -0.503, p= 0.095), making varieties with this haplotype (i.e. Chemlali Sfax and Meski) producing more saturated (C16: 0) and polyunsaturated acids than oleic acid. The latter plays a major role in preventing several diseases.

CONCLUSION

The two associations FADS2 FAD2.1 and FADS2 FAD2.3 with the fatty acid compositions of olive oil samples were identified among the studied olive cultivars. These associations differed between studied cultivars, which might explain variability in lipidic composition among them and consequently reflecting genetic diversity through differences in gene expression and biochemical pathways. FADS2 locus would constitute thus a good marker for detecting interesting lipidic chemotypes among commercial olive oils.

Authors+Show Affiliations

Molecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of Sfax, BP '1177' Sidi Mansour road, 3018, Sfax, Tunisia. raydabenayed@yahoo.fr.Molecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of Sfax, BP '1177' Sidi Mansour road, 3018, Sfax, Tunisia.Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.Laboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, PB 1177, 3018, Sfax, Tunisia.Laboratory of Extrêmophile Plants, Biotechnology Center of Borj-Cédria, B.P. 901, 2050, Hammam Lif, Tunisie.Laboratory of Extrêmophile Plants, Biotechnology Center of Borj-Cédria, B.P. 901, 2050, Hammam Lif, Tunisie.Ataturk University, Faculty of Agriculture, Department of Horticulture, 25240, Erzurum, Turkey.Molecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of Sfax, BP '1177' Sidi Mansour road, 3018, Sfax, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28701171

Citation

Ayed, Rayda Ben, et al. "Identification and Characterization of Single Nucleotide Polymorphism Markers in FADS2 Gene Associated With Olive Oil Fatty Acids Composition." Lipids in Health and Disease, vol. 16, no. 1, 2017, p. 138.
Ayed RB, Ennouri K, Hlima HB, et al. Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition. Lipids Health Dis. 2017;16(1):138.
Ayed, R. B., Ennouri, K., Hlima, H. B., Smaoui, S., Hanana, M., Mzid, R., Ercişli, S., & Rebai, A. (2017). Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition. Lipids in Health and Disease, 16(1), 138. https://doi.org/10.1186/s12944-017-0530-6
Ayed RB, et al. Identification and Characterization of Single Nucleotide Polymorphism Markers in FADS2 Gene Associated With Olive Oil Fatty Acids Composition. Lipids Health Dis. 2017 Jul 12;16(1):138. PubMed PMID: 28701171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition. AU - Ayed,Rayda Ben, AU - Ennouri,Karim, AU - Hlima,Hajer Ben, AU - Smaoui,Slim, AU - Hanana,Mohsen, AU - Mzid,Rim, AU - Ercişli,Sezai, AU - Rebai,Ahmed, Y1 - 2017/07/12/ PY - 2017/05/23/received PY - 2017/07/03/accepted PY - 2017/7/14/entrez PY - 2017/7/14/pubmed PY - 2018/5/1/medline KW - FADS2 KW - Fatty acid composition KW - Oleic acid KW - Olive oil KW - Quality KW - SNP genotype SP - 138 EP - 138 JF - Lipids in health and disease JO - Lipids Health Dis VL - 16 IS - 1 N2 - BACKGROUND: Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples. However, these associations require further confirmation in the Tunisian olive oil cultivars, and little is known about the effect of polymorphisms in fatty acid-related genes on olive oil mono- and poly- unsaturated fatty acids distribution. METHODS: A set of olive oils from 12 Tunisian cultivars was chosen. The fatty acid composition of each olive oil sample was determined by gas chromatography. Statistical and modeling Bayesian analyses were used to assess whether the FAD2.1 and FAD2.3 genotypes were associated with fatty acids composition. RESULTS: The TT-FAD2.1 and the GG-FAD2.3 genotypes were found to be associated with a lower proportion of oleic acid (C18:1) (r = -0.778, p = 0.003; r = -0.781, p= 0.003) as well as higher proportion of linoleic (C18:2) (r = 0.693, p = 0.012; r = -0.759, p= 0.004) and palmitic acids (C16:0) (r = 0.643, p = 0.024; r = -0.503, p= 0.095), making varieties with this haplotype (i.e. Chemlali Sfax and Meski) producing more saturated (C16: 0) and polyunsaturated acids than oleic acid. The latter plays a major role in preventing several diseases. CONCLUSION: The two associations FADS2 FAD2.1 and FADS2 FAD2.3 with the fatty acid compositions of olive oil samples were identified among the studied olive cultivars. These associations differed between studied cultivars, which might explain variability in lipidic composition among them and consequently reflecting genetic diversity through differences in gene expression and biochemical pathways. FADS2 locus would constitute thus a good marker for detecting interesting lipidic chemotypes among commercial olive oils. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/28701171/Identification_and_characterization_of_single_nucleotide_polymorphism_markers_in_FADS2_gene_associated_with_olive_oil_fatty_acids_composition_ L2 - https://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0530-6 DB - PRIME DP - Unbound Medicine ER -