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Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure.
Microbiol Mol Biol Rev. 2017 09; 81(3)MM

Abstract

The past 17 years have been marked by a revolution in our understanding of cellular multisubunit DNA-dependent RNA polymerases (MSDDRPs) at the structural level. A parallel development over the past 15 years has been the emerging story of the giant viruses, which encode MSDDRPs. Here we link the two in an attempt to understand the specialization of multisubunit RNA polymerases in the domain of life encompassing the large nucleocytoplasmic DNA viruses (NCLDV), a superclade that includes the giant viruses and the biochemically well-characterized poxvirus vaccinia virus. The first half of this review surveys the recently determined structural biology of cellular RNA polymerases for a microbiology readership. The second half discusses a reannotation of MSDDRP subunits from NCLDV families and the apparent specialization of these enzymes by virus family and by subunit with regard to subunit or domain loss, subunit dissociability, endogenous control of polymerase arrest, and the elimination/customization of regulatory interactions that would confer higher-order cellular control. Some themes are apparent in linking subunit function to structure in the viral world: as with cellular RNA polymerases I and III and unlike cellular RNA polymerase II, the viral enzymes seem to opt for speed and processivity and seem to have eliminated domains associated with higher-order regulation. The adoption/loss of viral RNA polymerase proofreading functions may have played a part in matching intrinsic mutability to genome size.

Authors+Show Affiliations

Department of Molecular Biology & Biochemisty, University of California-Irvine, Irvine, California, USA.Department of Molecular Biology & Biochemisty, University of California-Irvine, Irvine, California, USA pgershon@uci.edu.

Pub Type(s)

Journal Article
Review
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28701329

Citation

Mirzakhanyan, Yeva, and Paul D. Gershon. "Multisubunit DNA-Dependent RNA Polymerases From Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions From the Age of Structure." Microbiology and Molecular Biology Reviews : MMBR, vol. 81, no. 3, 2017.
Mirzakhanyan Y, Gershon PD. Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure. Microbiol Mol Biol Rev. 2017;81(3).
Mirzakhanyan, Y., & Gershon, P. D. (2017). Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure. Microbiology and Molecular Biology Reviews : MMBR, 81(3). https://doi.org/10.1128/MMBR.00010-17
Mirzakhanyan Y, Gershon PD. Multisubunit DNA-Dependent RNA Polymerases From Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions From the Age of Structure. Microbiol Mol Biol Rev. 2017;81(3) PubMed PMID: 28701329.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multisubunit DNA-Dependent RNA Polymerases from Vaccinia Virus and Other Nucleocytoplasmic Large-DNA Viruses: Impressions from the Age of Structure. AU - Mirzakhanyan,Yeva, AU - Gershon,Paul D, Y1 - 2017/07/12/ PY - 2017/7/14/entrez PY - 2017/7/14/pubmed PY - 2018/4/6/medline KW - NCLDV KW - RNA polymerase KW - giant virus KW - mimivirus KW - phycodnavirus KW - poxvirus KW - vaccinia virus JF - Microbiology and molecular biology reviews : MMBR JO - Microbiol Mol Biol Rev VL - 81 IS - 3 N2 - The past 17 years have been marked by a revolution in our understanding of cellular multisubunit DNA-dependent RNA polymerases (MSDDRPs) at the structural level. A parallel development over the past 15 years has been the emerging story of the giant viruses, which encode MSDDRPs. Here we link the two in an attempt to understand the specialization of multisubunit RNA polymerases in the domain of life encompassing the large nucleocytoplasmic DNA viruses (NCLDV), a superclade that includes the giant viruses and the biochemically well-characterized poxvirus vaccinia virus. The first half of this review surveys the recently determined structural biology of cellular RNA polymerases for a microbiology readership. The second half discusses a reannotation of MSDDRP subunits from NCLDV families and the apparent specialization of these enzymes by virus family and by subunit with regard to subunit or domain loss, subunit dissociability, endogenous control of polymerase arrest, and the elimination/customization of regulatory interactions that would confer higher-order cellular control. Some themes are apparent in linking subunit function to structure in the viral world: as with cellular RNA polymerases I and III and unlike cellular RNA polymerase II, the viral enzymes seem to opt for speed and processivity and seem to have eliminated domains associated with higher-order regulation. The adoption/loss of viral RNA polymerase proofreading functions may have played a part in matching intrinsic mutability to genome size. SN - 1098-5557 UR - https://www.unboundmedicine.com/medline/citation/28701329/Multisubunit_DNA_Dependent_RNA_Polymerases_from_Vaccinia_Virus_and_Other_Nucleocytoplasmic_Large_DNA_Viruses:_Impressions_from_the_Age_of_Structure_ L2 - http://mmbr.asm.org/cgi/pmidlookup?view=long&pmid=28701329 DB - PRIME DP - Unbound Medicine ER -