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Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection.
Virulence. 2017 10 03; 8(7):1417-1434.V

Abstract

Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MSE. In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensis yeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repressed cell wall biosynthesis. Bioinformatics and functional analyses also demonstrated that a serine proteinase was upregulated and secreted in vivo. To our knowledge this is the first study depicting transcriptional and proteomic data of P. brasiliensis yeast cells upon 6 h post-infection of mouse lung.

Authors+Show Affiliations

a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.b Laboratório de Patologia , Escola de Veterinária e Zootecnia, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.b Laboratório de Patologia , Escola de Veterinária e Zootecnia, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.c Instituto René Rachou, Fundação Oswaldo Cruz , Belo Horizonte , Minas Gerais , Brazil.d Department of Medicine , University of Wisconsin , Madison , WI , USA.a Laboratório de Biologia Molecular , Instituto de Ciências Biológicas, Universidade Federal de Goiás , Campus Samambaia s/n, Goiânia , Goiás , Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28704618

Citation

Lacerda Pigosso, Laurine, et al. "Paracoccidioides Brasiliensis Presents Metabolic Reprogramming and Secretes a Serine Proteinase During Murine Infection." Virulence, vol. 8, no. 7, 2017, pp. 1417-1434.
Lacerda Pigosso L, Baeza LC, Vieira Tomazett M, et al. Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection. Virulence. 2017;8(7):1417-1434.
Lacerda Pigosso, L., Baeza, L. C., Vieira Tomazett, M., Batista Rodrigues Faleiro, M., Brianezi Dignani de Moura, V. M., Melo Bailão, A., Borges, C. L., Alves Parente Rocha, J., Rocha Fernandes, G., Gauthier, G. M., & Soares, C. M. A. (2017). Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection. Virulence, 8(7), 1417-1434. https://doi.org/10.1080/21505594.2017.1355660
Lacerda Pigosso L, et al. Paracoccidioides Brasiliensis Presents Metabolic Reprogramming and Secretes a Serine Proteinase During Murine Infection. Virulence. 2017 10 3;8(7):1417-1434. PubMed PMID: 28704618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection. AU - Lacerda Pigosso,Laurine, AU - Baeza,Lilian Cristiane, AU - Vieira Tomazett,Mariana, AU - Batista Rodrigues Faleiro,Mariana, AU - Brianezi Dignani de Moura,Veridiana Maria, AU - Melo Bailão,Alexandre, AU - Borges,Clayton Luiz, AU - Alves Parente Rocha,Juliana, AU - Rocha Fernandes,Gabriel, AU - Gauthier,Gregory M, AU - Soares,Celia Maria de Almeida, Y1 - 2017/07/13/ PY - 2017/7/14/pubmed PY - 2018/3/20/medline PY - 2017/7/14/entrez KW - Paracoccidioides brasiliensis KW - metabolic adaptation KW - proteome KW - serine proteinase KW - transcriptome SP - 1417 EP - 1434 JF - Virulence JO - Virulence VL - 8 IS - 7 N2 - Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MSE. In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensis yeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repressed cell wall biosynthesis. Bioinformatics and functional analyses also demonstrated that a serine proteinase was upregulated and secreted in vivo. To our knowledge this is the first study depicting transcriptional and proteomic data of P. brasiliensis yeast cells upon 6 h post-infection of mouse lung. SN - 2150-5608 UR - https://www.unboundmedicine.com/medline/citation/28704618/Paracoccidioides_brasiliensis_presents_metabolic_reprogramming_and_secretes_a_serine_proteinase_during_murine_infection_ L2 - http://www.tandfonline.com/doi/full/10.1080/21505594.2017.1355660 DB - PRIME DP - Unbound Medicine ER -