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Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus.
Fish Shellfish Immunol. 2017 Sep; 68:232-242.FS

Abstract

A feeding experiment was conducted to investigate the effects of dietary administration of synbiotic with Bacillus lincheniformis WS-2 (CGMCC No. 12813) and alginate oligosaccharides (AOS) on the growth, innate immune response, and intestinal microbiota of the sea cucumber Apostichopus japonicus and its resistance to Vibrio infection. Sea cucumbers were given a control diet (non-supplemented), pro diet (basal diet plus 1 × 109 cfu (g diet)-1B. lincheniformis WS-2), syn diet (basal diet plus 1 × 109 cfu (g diet)-1B. lincheniformis WS-2 and 10 g (kg diet) -1 AOS) or pre diet (basal diet plus 10 g (kg diet) -1 AOS) over a period of 60 days, and the growth performance and various innate immune parameters of the animals were evaluated after 30 and 60 days of feeding. No significant difference in growth performance was observed between the group fed with the syn and the group fed with the pro diet, but both these groups exhibited significant (P < 0.05) enhancement in growth performance compared to the control group. At the same time, both syn and pro diets also resulted in the animals having significantly higher levels of amylase, protease and alginate lyase activities compared to the con diet. Individuals fed with the syn or pro diet showed enhanced levels of various immune enzyme activities, compared to those fed with the con diet. At the end of the growth period, the sea cucumbers were challenged with Vibrio splendidus via intraperitoneal injection. The survival rates of sea cucumbers fed with the syn, pro or pre diet were significantly improved compared to that of sea cucumbers fed with the con diet, with sea cucumbers fed with synbiotic having the highest survival. In addition, increased proportions of Bacillus and Lactococcus were found in the intestinal tract of sea cucumbers fed with the syn diet (9.5% and 7.3%) compared to those of sea cucumbers fed with the pro diet (6.1% and 4.6%), con diet (4.0% and 3.4%), or pre diet (5.2% and 6.8%) after 60 days of feeding. Furthermore, the proportion of Vibrio in the intestinal tracts of sea cucumbers fed with the pro diet (2%) or syn diet (3.1%) was lower than that of sea cucumbers fed with the con diet (5.5%) or pre diet (3.8%), although no significant difference was detected between the pro diet and syn diet groups (P > 0.05). Overall, the results suggested that dietary synbiotic consisting of Bacillus lincheniformis and alginate oligosaccharides (AOS) could have positive benefit for sea cucumber aquaculture.

Authors+Show Affiliations

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China.Dalian Biotechnology Research Institute, Liaoning Academy of Agricultural Sciences, Dalian 116024, People's Republic of China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China; Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, People's Republic of China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, People's Republic of China; Ministry of Education Center for Food Safety of Animal Origin, Dalian University of Technology, Dalian 116620, People's Republic of China. Electronic address: wxt731@sina.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28709723

Citation

Wang, Xitao, et al. "Synbiotic Dietary Supplement Affects Growth, Immune Responses and Intestinal Microbiota of Apostichopus Japonicus." Fish & Shellfish Immunology, vol. 68, 2017, pp. 232-242.
Wang X, Sun Y, Wang L, et al. Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus. Fish Shellfish Immunol. 2017;68:232-242.
Wang, X., Sun, Y., Wang, L., Li, X., Qu, K., & Xu, Y. (2017). Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus. Fish & Shellfish Immunology, 68, 232-242. https://doi.org/10.1016/j.fsi.2017.07.027
Wang X, et al. Synbiotic Dietary Supplement Affects Growth, Immune Responses and Intestinal Microbiota of Apostichopus Japonicus. Fish Shellfish Immunol. 2017;68:232-242. PubMed PMID: 28709723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus. AU - Wang,Xitao, AU - Sun,Yongxin, AU - Wang,Lili, AU - Li,Xiaoyu, AU - Qu,Kunli, AU - Xu,Yongping, Y1 - 2017/07/11/ PY - 2017/01/07/received PY - 2017/06/20/revised PY - 2017/07/10/accepted PY - 2017/7/16/pubmed PY - 2018/6/7/medline PY - 2017/7/16/entrez KW - Apostichopus japonicus KW - Intestinal microbiome KW - Prebiotic KW - Probiotic KW - Synbiotic SP - 232 EP - 242 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 68 N2 - A feeding experiment was conducted to investigate the effects of dietary administration of synbiotic with Bacillus lincheniformis WS-2 (CGMCC No. 12813) and alginate oligosaccharides (AOS) on the growth, innate immune response, and intestinal microbiota of the sea cucumber Apostichopus japonicus and its resistance to Vibrio infection. Sea cucumbers were given a control diet (non-supplemented), pro diet (basal diet plus 1 × 109 cfu (g diet)-1B. lincheniformis WS-2), syn diet (basal diet plus 1 × 109 cfu (g diet)-1B. lincheniformis WS-2 and 10 g (kg diet) -1 AOS) or pre diet (basal diet plus 10 g (kg diet) -1 AOS) over a period of 60 days, and the growth performance and various innate immune parameters of the animals were evaluated after 30 and 60 days of feeding. No significant difference in growth performance was observed between the group fed with the syn and the group fed with the pro diet, but both these groups exhibited significant (P < 0.05) enhancement in growth performance compared to the control group. At the same time, both syn and pro diets also resulted in the animals having significantly higher levels of amylase, protease and alginate lyase activities compared to the con diet. Individuals fed with the syn or pro diet showed enhanced levels of various immune enzyme activities, compared to those fed with the con diet. At the end of the growth period, the sea cucumbers were challenged with Vibrio splendidus via intraperitoneal injection. The survival rates of sea cucumbers fed with the syn, pro or pre diet were significantly improved compared to that of sea cucumbers fed with the con diet, with sea cucumbers fed with synbiotic having the highest survival. In addition, increased proportions of Bacillus and Lactococcus were found in the intestinal tract of sea cucumbers fed with the syn diet (9.5% and 7.3%) compared to those of sea cucumbers fed with the pro diet (6.1% and 4.6%), con diet (4.0% and 3.4%), or pre diet (5.2% and 6.8%) after 60 days of feeding. Furthermore, the proportion of Vibrio in the intestinal tracts of sea cucumbers fed with the pro diet (2%) or syn diet (3.1%) was lower than that of sea cucumbers fed with the con diet (5.5%) or pre diet (3.8%), although no significant difference was detected between the pro diet and syn diet groups (P > 0.05). Overall, the results suggested that dietary synbiotic consisting of Bacillus lincheniformis and alginate oligosaccharides (AOS) could have positive benefit for sea cucumber aquaculture. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/28709723/Synbiotic_dietary_supplement_affects_growth_immune_responses_and_intestinal_microbiota_of_Apostichopus_japonicus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(17)30421-7 DB - PRIME DP - Unbound Medicine ER -