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Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a.
Oncol Rep 2017; 38(3):1895-1901OR

Abstract

Spica Prunellae is the spike of the herb Prunella vulgaris L. in traditional Chinese medicine which is often used for the treatment of various cancers including colorectal cancer. In the present study, we found that a key tumor suppressor, microRNA-34a (miR-34a) is involved in the antitumor activity for Spica Prunellae. Human colon carcinoma HCT-8 cells treated with an ethanol extract of Spica Prunellae (EESP) had significantly decreased cell proliferation and viability, in a dose-dependent manner. Flow cytometry analysis with Annexin V/PI staining analysis revealed that EESP treatment could induce apoptosis of HCT-8 cells. The level of miR-34a was upregulated in HCT-8 cells following EESP treatment, whereas expression levels of its target genes Notch1, Notch2 and Bcl-2 were downregulated. Inhibition of miR-34a rescued the expression of these target genes. These results revealed that Spica Prunellae can suppress the growth of HCT-8 cells by targeting Notch1, Notch2 and Bcl-2 via activation of miR-34a.

Authors+Show Affiliations

Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28713966

Citation

Fang, Yi, et al. "Spica Prunellae Extract Suppresses the Growth of Human Colon Carcinoma Cells By Targeting Multiple Oncogenes Via Activating MiR-34a." Oncology Reports, vol. 38, no. 3, 2017, pp. 1895-1901.
Fang Y, Zhang L, Feng J, et al. Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncol Rep. 2017;38(3):1895-1901.
Fang, Y., Zhang, L., Feng, J., Lin, W., Cai, Q., & Peng, J. (2017). Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. Oncology Reports, 38(3), pp. 1895-1901. doi:10.3892/or.2017.5792.
Fang Y, et al. Spica Prunellae Extract Suppresses the Growth of Human Colon Carcinoma Cells By Targeting Multiple Oncogenes Via Activating MiR-34a. Oncol Rep. 2017;38(3):1895-1901. PubMed PMID: 28713966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spica Prunellae extract suppresses the growth of human colon carcinoma cells by targeting multiple oncogenes via activating miR-34a. AU - Fang,Yi, AU - Zhang,Ling, AU - Feng,Jianyu, AU - Lin,Wei, AU - Cai,Qiaoyan, AU - Peng,Jun, Y1 - 2017/07/06/ PY - 2017/01/19/received PY - 2017/06/19/accepted PY - 2017/7/18/pubmed PY - 2018/4/24/medline PY - 2017/7/18/entrez SP - 1895 EP - 1901 JF - Oncology reports JO - Oncol. Rep. VL - 38 IS - 3 N2 - Spica Prunellae is the spike of the herb Prunella vulgaris L. in traditional Chinese medicine which is often used for the treatment of various cancers including colorectal cancer. In the present study, we found that a key tumor suppressor, microRNA-34a (miR-34a) is involved in the antitumor activity for Spica Prunellae. Human colon carcinoma HCT-8 cells treated with an ethanol extract of Spica Prunellae (EESP) had significantly decreased cell proliferation and viability, in a dose-dependent manner. Flow cytometry analysis with Annexin V/PI staining analysis revealed that EESP treatment could induce apoptosis of HCT-8 cells. The level of miR-34a was upregulated in HCT-8 cells following EESP treatment, whereas expression levels of its target genes Notch1, Notch2 and Bcl-2 were downregulated. Inhibition of miR-34a rescued the expression of these target genes. These results revealed that Spica Prunellae can suppress the growth of HCT-8 cells by targeting Notch1, Notch2 and Bcl-2 via activation of miR-34a. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/28713966/Spica_Prunellae_extract_suppresses_the_growth_of_human_colon_carcinoma_cells_by_targeting_multiple_oncogenes_via_activating_miR_34a_ L2 - http://www.spandidos-publications.com/or/38/3/1895 DB - PRIME DP - Unbound Medicine ER -