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microRNA159-targeted SlGAMYB transcription factors are required for fruit set in tomato.
Plant J. 2017 Oct; 92(1):95-109.PJ

Abstract

The transition from flowering to fruit production, namely fruit set, is crucial to ensure successful sexual plant reproduction. Although studies have described the importance of hormones (i.e. auxin and gibberellins) in controlling fruit set after pollination and fertilization, the role of microRNA-based regulation during ovary development and fruit set is still poorly understood. Here we show that the microRNA159/GAMYB1 and -2 pathway (the miR159/GAMYB1/2 module) is crucial for tomato ovule development and fruit set. MiR159 and SlGAMYBs were expressed in preanthesis ovaries, mainly in meristematic tissues, including developing ovules. SlMIR159-overexpressing tomato cv. Micro-Tom plants exhibited precocious fruit initiation and obligatory parthenocarpy, without modifying fruit shape. Histological analysis showed abnormal ovule development in such plants, which led to the formation of seedless fruits. SlGAMYB1/2 silencing in SlMIR159-overexpressing plants resulted in misregulation of pathways associated with ovule and female gametophyte development and auxin signalling, including AINTEGUMENTA-like genes and the miR167/SlARF8a module. Similarly to SlMIR159-overexpressing plants, SlGAMYB1 was downregulated in ovaries of parthenocarpic mutants with altered responses to gibberellins and auxin. SlGAMYBs likely contribute to fruit initiation by modulating auxin and gibberellin responses, rather than their levels, during ovule and ovary development. Altogether, our results unveil a novel function for the miR159-targeted SlGAMYBs in regulating an agronomically important trait, namely fruit set.

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Authors+Show Affiliations

da Silva EM
Bioscience Institute, State University of Sao Paulo, Botucatu, Sao Paulo, 18618-970, Brazil. Laboratory of Molecular Genetics of Plant Development, Department of Biological Sciences, Escola Superior de Agricultura 'Luiz de Queiroz', University of Sao Paulo, Piracicaba, Sao Paulo, 13418-900, Brazil.
Silva GFFE
Laboratory of Molecular Genetics of Plant Development, Department of Biological Sciences, Escola Superior de Agricultura 'Luiz de Queiroz', University of Sao Paulo, Piracicaba, Sao Paulo, 13418-900, Brazil.
Bidoia DB
Bioscience Institute, State University of Sao Paulo, Botucatu, Sao Paulo, 18618-970, Brazil. Laboratory of Molecular Genetics of Plant Development, Department of Biological Sciences, Escola Superior de Agricultura 'Luiz de Queiroz', University of Sao Paulo, Piracicaba, Sao Paulo, 13418-900, Brazil.
da Silva Azevedo M
Laboratory of Hormonal Control of Plant Development, Department of Biological Sciences (LCB), Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of Sao Paulo (USP), Piracicaba, Sao Paulo, 13418-900, Brazil.
de Jesus FA
Laboratory of Hormonal Control of Plant Development, Department of Biological Sciences (LCB), Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of Sao Paulo (USP), Piracicaba, Sao Paulo, 13418-900, Brazil.
Pino LE
Laboratory of Hormonal Control of Plant Development, Department of Biological Sciences (LCB), Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of Sao Paulo (USP), Piracicaba, Sao Paulo, 13418-900, Brazil.
Peres LEP
Laboratory of Hormonal Control of Plant Development, Department of Biological Sciences (LCB), Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), University of Sao Paulo (USP), Piracicaba, Sao Paulo, 13418-900, Brazil.
Carrera E
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Ingeniero Fausto Elío s/n, Valencia, 46022, Spain.
López-Díaz I
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Ingeniero Fausto Elío s/n, Valencia, 46022, Spain.
Nogueira FTS
Laboratory of Molecular Genetics of Plant Development, Department of Biological Sciences, Escola Superior de Agricultura 'Luiz de Queiroz', University of Sao Paulo, Piracicaba, Sao Paulo, 13418-900, Brazil.

MeSH

Down-RegulationFlowersFruitGene Expression Regulation, PlantGibberellinsIndoleacetic AcidsLycopersicon esculentumMicroRNAsOvulePlant Growth RegulatorsPlant ProteinsPollinationRNA, PlantTranscription Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28715118

Citation

da Silva, Eder Marques, et al. "MicroRNA159-targeted SlGAMYB Transcription Factors Are Required for Fruit Set in Tomato." The Plant Journal : for Cell and Molecular Biology, vol. 92, no. 1, 2017, pp. 95-109.
da Silva EM, Silva GFFE, Bidoia DB, et al. MicroRNA159-targeted SlGAMYB transcription factors are required for fruit set in tomato. Plant J. 2017;92(1):95-109.
da Silva, E. M., Silva, G. F. F. E., Bidoia, D. B., da Silva Azevedo, M., de Jesus, F. A., Pino, L. E., Peres, L. E. P., Carrera, E., López-Díaz, I., & Nogueira, F. T. S. (2017). MicroRNA159-targeted SlGAMYB transcription factors are required for fruit set in tomato. The Plant Journal : for Cell and Molecular Biology, 92(1), 95-109. https://doi.org/10.1111/tpj.13637
da Silva EM, et al. MicroRNA159-targeted SlGAMYB Transcription Factors Are Required for Fruit Set in Tomato. Plant J. 2017;92(1):95-109. PubMed PMID: 28715118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - microRNA159-targeted SlGAMYB transcription factors are required for fruit set in tomato. AU - da Silva,Eder Marques, AU - Silva,Geraldo Felipe Ferreira E, AU - Bidoia,Débora Brussolo, AU - da Silva Azevedo,Mariana, AU - de Jesus,Frederico Almeida, AU - Pino,Lilian Ellen, AU - Peres,Lázaro Eustáquio Pereira, AU - Carrera,Esther, AU - López-Díaz,Isabel, AU - Nogueira,Fabio Tebaldi Silveira, Y1 - 2017/08/21/ PY - 2017/02/07/received PY - 2017/06/27/revised PY - 2017/07/10/accepted PY - 2017/7/18/pubmed PY - 2018/3/27/medline PY - 2017/7/18/entrez KW - GAMYB KW - Solanum lycopersicum KW - miR159 KW - ovary KW - parthenocarpy SP - 95 EP - 109 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 92 IS - 1 N2 - The transition from flowering to fruit production, namely fruit set, is crucial to ensure successful sexual plant reproduction. Although studies have described the importance of hormones (i.e. auxin and gibberellins) in controlling fruit set after pollination and fertilization, the role of microRNA-based regulation during ovary development and fruit set is still poorly understood. Here we show that the microRNA159/GAMYB1 and -2 pathway (the miR159/GAMYB1/2 module) is crucial for tomato ovule development and fruit set. MiR159 and SlGAMYBs were expressed in preanthesis ovaries, mainly in meristematic tissues, including developing ovules. SlMIR159-overexpressing tomato cv. Micro-Tom plants exhibited precocious fruit initiation and obligatory parthenocarpy, without modifying fruit shape. Histological analysis showed abnormal ovule development in such plants, which led to the formation of seedless fruits. SlGAMYB1/2 silencing in SlMIR159-overexpressing plants resulted in misregulation of pathways associated with ovule and female gametophyte development and auxin signalling, including AINTEGUMENTA-like genes and the miR167/SlARF8a module. Similarly to SlMIR159-overexpressing plants, SlGAMYB1 was downregulated in ovaries of parthenocarpic mutants with altered responses to gibberellins and auxin. SlGAMYBs likely contribute to fruit initiation by modulating auxin and gibberellin responses, rather than their levels, during ovule and ovary development. Altogether, our results unveil a novel function for the miR159-targeted SlGAMYBs in regulating an agronomically important trait, namely fruit set. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/28715118/microRNA159_targeted_SlGAMYB_transcription_factors_are_required_for_fruit_set_in_tomato_ L2 - https://doi.org/10.1111/tpj.13637 DB - PRIME DP - Unbound Medicine ER -