Tags

Type your tag names separated by a space and hit enter

[Dietary fatty acids and delta 5 desaturation by rat liver microsomes].
Reprod Nutr Dev. 1986; 26(1A):77-84.RN

Abstract

This paper reports a comparative study of the influence of dietary fats on the delta 5 desaturation of dihomo-gamma-linolenic acid (20:3 n-6) to arachidonic acid (20:4 n-6) using rat liver microsomes. The kinetic conditions needed to measure the specific activity of desaturation have been described. The rate of delta 5 desaturation was studied at two substrate levels. In a first series of experiments, the liver microsomes of animals maintained on a chow diet showed less desaturation activity than those of animals fed a fat-free diet. For longer periods of control (fat-free) diet time, the amount of 20:4 n-6 produced in vitro was depressed and the 20:3 n-9/20:4 n-6 ratio in total lipids was enhanced. In a second series of experiments, groups of rats were fed semi-synthetic diets which contained 5 or 10% of dietary fats with different percentages of linoleic acid (18:2 n-6), alpha-linolenic acid (18:3 n-3), oleic acid (18:1 n-9) or saturated fatty acids. Liver microsomal delta 5 desaturase activities that were generally depressed, when compared with control rats, were moderately affected by dietary fats, except with a diet containing saturated fatty acids or alpha-linolenic acid for which delta 5 desaturation was decreased. These observations are discussed in relation with the recent literature.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

fre

PubMed ID

2871600

Citation

Blond, J P., et al. "[Dietary Fatty Acids and Delta 5 Desaturation By Rat Liver Microsomes]." Reproduction, Nutrition, Developpement, vol. 26, no. 1A, 1986, pp. 77-84.
Blond JP, Ratieuville P, Bézard J. [Dietary fatty acids and delta 5 desaturation by rat liver microsomes]. Reprod Nutr Dev. 1986;26(1A):77-84.
Blond, J. P., Ratieuville, P., & Bézard, J. (1986). [Dietary fatty acids and delta 5 desaturation by rat liver microsomes]. Reproduction, Nutrition, Developpement, 26(1A), 77-84.
Blond JP, Ratieuville P, Bézard J. [Dietary Fatty Acids and Delta 5 Desaturation By Rat Liver Microsomes]. Reprod Nutr Dev. 1986;26(1A):77-84. PubMed PMID: 2871600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Dietary fatty acids and delta 5 desaturation by rat liver microsomes]. AU - Blond,J P, AU - Ratieuville,P, AU - Bézard,J, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 77 EP - 84 JF - Reproduction, nutrition, developpement JO - Reprod Nutr Dev VL - 26 IS - 1A N2 - This paper reports a comparative study of the influence of dietary fats on the delta 5 desaturation of dihomo-gamma-linolenic acid (20:3 n-6) to arachidonic acid (20:4 n-6) using rat liver microsomes. The kinetic conditions needed to measure the specific activity of desaturation have been described. The rate of delta 5 desaturation was studied at two substrate levels. In a first series of experiments, the liver microsomes of animals maintained on a chow diet showed less desaturation activity than those of animals fed a fat-free diet. For longer periods of control (fat-free) diet time, the amount of 20:4 n-6 produced in vitro was depressed and the 20:3 n-9/20:4 n-6 ratio in total lipids was enhanced. In a second series of experiments, groups of rats were fed semi-synthetic diets which contained 5 or 10% of dietary fats with different percentages of linoleic acid (18:2 n-6), alpha-linolenic acid (18:3 n-3), oleic acid (18:1 n-9) or saturated fatty acids. Liver microsomal delta 5 desaturase activities that were generally depressed, when compared with control rats, were moderately affected by dietary fats, except with a diet containing saturated fatty acids or alpha-linolenic acid for which delta 5 desaturation was decreased. These observations are discussed in relation with the recent literature. SN - 0181-1916 UR - https://www.unboundmedicine.com/medline/citation/2871600/[Dietary_fatty_acids_and_delta_5_desaturation_by_rat_liver_microsomes]_ L2 - https://medlineplus.gov/dietaryfats.html DB - PRIME DP - Unbound Medicine ER -