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Specific anti-Bordetella pertussis activities in human blood examined by enzyme-linked immunoassay and biological assay.
Dev Biol Stand. 1985; 61:341-52.DB

Abstract

Enzyme-linked immunoassays have been employed in this work to measure antibodies against either pertussigen or filamentous hemagglutinin, using a urease-conjugated second antibody system. Pertussigen and filamentous hemagglutinin were obtained from the supernatants of Bordetella pertussis cultures by chromatographic procedures, and were shown to be essentially free of other proteins. Further checks have established the specificity of the enzyme-linked immunoassay-systems. Anti-pertussigen and anti-filamentous hemagglutinin antibody titers, in a pilot study of sera from normal adults, showed little correlation with the donors' pertussis contact, infection or vaccination histories. Nevertheless, the titers did correlate with the ability of the sera to neutralize the biological actions of pertussigen in mice and the haemagglutinating activity of filamentous haemagglutinin, respectively. Antibody titers of sera from patients with suspected or confirmed pertussis infection fell within the range covered by sera from normal adults. Sera from unvaccinated children (less than two months old) also showed some anti-pertussis activity that was probably maternal in origin. Further selected adult plasma samples have been compared for anti-pertussis activity by enzyme-linked immunoassays and agglutination. These samples also have been evaluated for their abilities to protect mice passively against intra-nasal and intra-cerebral challenge with Bordetella pertussis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2872123

Citation

Marley, P B., et al. "Specific anti-Bordetella Pertussis Activities in Human Blood Examined By Enzyme-linked Immunoassay and Biological Assay." Developments in Biological Standardization, vol. 61, 1985, pp. 341-52.
Marley PB, Wilson JV, Habel H, et al. Specific anti-Bordetella pertussis activities in human blood examined by enzyme-linked immunoassay and biological assay. Dev Biol Stand. 1985;61:341-52.
Marley, P. B., Wilson, J. V., Habel, H., & Blaskett, A. (1985). Specific anti-Bordetella pertussis activities in human blood examined by enzyme-linked immunoassay and biological assay. Developments in Biological Standardization, 61, 341-52.
Marley PB, et al. Specific anti-Bordetella Pertussis Activities in Human Blood Examined By Enzyme-linked Immunoassay and Biological Assay. Dev Biol Stand. 1985;61:341-52. PubMed PMID: 2872123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specific anti-Bordetella pertussis activities in human blood examined by enzyme-linked immunoassay and biological assay. AU - Marley,P B, AU - Wilson,J V, AU - Habel,H, AU - Blaskett,A, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 341 EP - 52 JF - Developments in biological standardization JO - Dev Biol Stand VL - 61 N2 - Enzyme-linked immunoassays have been employed in this work to measure antibodies against either pertussigen or filamentous hemagglutinin, using a urease-conjugated second antibody system. Pertussigen and filamentous hemagglutinin were obtained from the supernatants of Bordetella pertussis cultures by chromatographic procedures, and were shown to be essentially free of other proteins. Further checks have established the specificity of the enzyme-linked immunoassay-systems. Anti-pertussigen and anti-filamentous hemagglutinin antibody titers, in a pilot study of sera from normal adults, showed little correlation with the donors' pertussis contact, infection or vaccination histories. Nevertheless, the titers did correlate with the ability of the sera to neutralize the biological actions of pertussigen in mice and the haemagglutinating activity of filamentous haemagglutinin, respectively. Antibody titers of sera from patients with suspected or confirmed pertussis infection fell within the range covered by sera from normal adults. Sera from unvaccinated children (less than two months old) also showed some anti-pertussis activity that was probably maternal in origin. Further selected adult plasma samples have been compared for anti-pertussis activity by enzyme-linked immunoassays and agglutination. These samples also have been evaluated for their abilities to protect mice passively against intra-nasal and intra-cerebral challenge with Bordetella pertussis. SN - 0301-5149 UR - https://www.unboundmedicine.com/medline/citation/2872123/Specific_anti_Bordetella_pertussis_activities_in_human_blood_examined_by_enzyme_linked_immunoassay_and_biological_assay_ DB - PRIME DP - Unbound Medicine ER -