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Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice.
Biotechnol Lett. 2017 Oct; 39(10):1521-1527.BL

Abstract

OBJECTIVES

To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions.

RESULTS

The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%. Quantitative RT-PCR assays were performed to investigate the gene expression profiles of S. cerevisiae KKU-VN8. Differential expression of genes encoding heat-shock proteins (HSP82, HSP104, SSA4), genes involved in trehalose metabolism (TPS1, TPS2, NTH1) and genes involved the glycolytic pathway (ADH1, ADH2, CDC19) at various time points during fermentation was observed. The expression levels of HSP82, HSP104, SSA4, ADH1 and CDC19 were significantly higher than those of the controls (10.2-, 4-, 8-, 8.9- and 5.9-fold higher, respectively). In contrast, the expression levels of TPS1, TPS2, NTH1 and ADH2 were approx. 2-fold less than those of the controls.

CONCLUSIONS

The highly expressed genes encoding heat-shock proteins, HSP82 and SSA4, potentially play an important role in helping S. cerevisiae KKU-VN8 cope with various stresses that occur during high-temperature fermentation, leading to higher ethanol production efficiency.

Authors+Show Affiliations

Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand.Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand. Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen, 40002, Thailand.Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand. kpreek@kku.ac.th. Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen, 40002, Thailand. kpreek@kku.ac.th.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28721580

Citation

Techaparin, Atiya, et al. "Gene Expression Profiles of the Thermotolerant Yeast Saccharomyces Cerevisiae Strain KKU-VN8 During High-temperature Ethanol Fermentation Using Sweet Sorghum Juice." Biotechnology Letters, vol. 39, no. 10, 2017, pp. 1521-1527.
Techaparin A, Thanonkeo P, Klanrit P. Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. Biotechnol Lett. 2017;39(10):1521-1527.
Techaparin, A., Thanonkeo, P., & Klanrit, P. (2017). Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. Biotechnology Letters, 39(10), 1521-1527. https://doi.org/10.1007/s10529-017-2398-y
Techaparin A, Thanonkeo P, Klanrit P. Gene Expression Profiles of the Thermotolerant Yeast Saccharomyces Cerevisiae Strain KKU-VN8 During High-temperature Ethanol Fermentation Using Sweet Sorghum Juice. Biotechnol Lett. 2017;39(10):1521-1527. PubMed PMID: 28721580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. AU - Techaparin,Atiya, AU - Thanonkeo,Pornthap, AU - Klanrit,Preekamol, Y1 - 2017/07/18/ PY - 2017/05/06/received PY - 2017/07/13/accepted PY - 2017/7/20/pubmed PY - 2018/7/11/medline PY - 2017/7/20/entrez KW - Gene expression KW - Glycolytic pathway KW - Heat-shock proteins KW - Saccharomyces cerevisiae KW - Sweet sorghum juice KW - Trehalose metabolism SP - 1521 EP - 1527 JF - Biotechnology letters JO - Biotechnol Lett VL - 39 IS - 10 N2 - OBJECTIVES: To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS: The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%. Quantitative RT-PCR assays were performed to investigate the gene expression profiles of S. cerevisiae KKU-VN8. Differential expression of genes encoding heat-shock proteins (HSP82, HSP104, SSA4), genes involved in trehalose metabolism (TPS1, TPS2, NTH1) and genes involved the glycolytic pathway (ADH1, ADH2, CDC19) at various time points during fermentation was observed. The expression levels of HSP82, HSP104, SSA4, ADH1 and CDC19 were significantly higher than those of the controls (10.2-, 4-, 8-, 8.9- and 5.9-fold higher, respectively). In contrast, the expression levels of TPS1, TPS2, NTH1 and ADH2 were approx. 2-fold less than those of the controls. CONCLUSIONS: The highly expressed genes encoding heat-shock proteins, HSP82 and SSA4, potentially play an important role in helping S. cerevisiae KKU-VN8 cope with various stresses that occur during high-temperature fermentation, leading to higher ethanol production efficiency. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/28721580/Gene_expression_profiles_of_the_thermotolerant_yeast_Saccharomyces_cerevisiae_strain_KKU_VN8_during_high_temperature_ethanol_fermentation_using_sweet_sorghum_juice_ L2 - https://doi.org/10.1007/s10529-017-2398-y DB - PRIME DP - Unbound Medicine ER -