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In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based on Freestanding Nanohybrid Paper Electrode Assembled from 3D Functionalized Graphene Framework.
ACS Appl Mater Interfaces. 2017 Nov 08; 9(44):38201-38210.AA

Abstract

In this work, we develop a new type of freestanding nanohybrid paper electrode assembled from 3D ionic liquid (IL) functionalized graphene framework (GF) decorated by gold nanoflowers (AuNFs), and explore its practical application in in situ electrochemical sensing of live breast cell samples by real-time tracking biomarker H2O2 released from cells. The AuNFs modified IL functionalized GF (AuNFs/IL-GF) was synthesized via a facile and efficient dopamine-assisted one-pot self-assembly strategy. The as-obtained nanohybrid assembly exhibits a typical 3D hierarchical porous structure, where the highly active electrocatalyst AuNFs are well dispersed on IL-GF scaffold. And the graft of hydrophilic IL molecules (i.e., 1-butyl-3-methylimidazolium tetrafluoroborate, BMIMBF4) on graphene nanosheets not only avoids their agglomeration and disorder stacking during the self-assembly but also endows the integrated IL-GF monolithic material with unique hydrophilic properties, which enables it to be readily dispersed in aqueous solution and processed into freestanding paperlike material. Because of the unique structural properties and the combinational advantages of different components in the AuNFs/IL-GF composite, the resultant nanohybrid paper electrode exhibits good nonenzymatic electrochemical sensing performance toward H2O2. When used in real-time tracking H2O2 secreted from different breast cells attached to the paper electrode without or with radiotherapy treatment, the proposed electrochemical sensor based on freestanding AuNFs/IL-GF paper electrode can distinguish the normal breast cell HBL-100 from the cancer breast cells MDA-MB-231 and MCF-7 cells, and assess the radiotherapy effects to different breast cancer cells, which opens a new horizon in real-time monitoring cancer cells by electrochemical sensing platform.

Authors+Show Affiliations

Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, P. R. China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, P. R. China.Hubei key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology , Wuhan 430073, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, P. R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28727416

Citation

Zhang, Yan, et al. "In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based On Freestanding Nanohybrid Paper Electrode Assembled From 3D Functionalized Graphene Framework." ACS Applied Materials & Interfaces, vol. 9, no. 44, 2017, pp. 38201-38210.
Zhang Y, Xiao J, Lv Q, et al. In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based on Freestanding Nanohybrid Paper Electrode Assembled from 3D Functionalized Graphene Framework. ACS Appl Mater Interfaces. 2017;9(44):38201-38210.
Zhang, Y., Xiao, J., Lv, Q., Wang, L., Dong, X., Asif, M., Ren, J., He, W., Sun, Y., Xiao, F., & Wang, S. (2017). In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based on Freestanding Nanohybrid Paper Electrode Assembled from 3D Functionalized Graphene Framework. ACS Applied Materials & Interfaces, 9(44), 38201-38210. https://doi.org/10.1021/acsami.7b08781
Zhang Y, et al. In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based On Freestanding Nanohybrid Paper Electrode Assembled From 3D Functionalized Graphene Framework. ACS Appl Mater Interfaces. 2017 Nov 8;9(44):38201-38210. PubMed PMID: 28727416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based on Freestanding Nanohybrid Paper Electrode Assembled from 3D Functionalized Graphene Framework. AU - Zhang,Yan, AU - Xiao,Jian, AU - Lv,Qiying, AU - Wang,Lu, AU - Dong,Xulin, AU - Asif,Muhammad, AU - Ren,Jinghua, AU - He,Wenshan, AU - Sun,Yimin, AU - Xiao,Fei, AU - Wang,Shuai, Y1 - 2017/10/24/ PY - 2017/7/21/pubmed PY - 2018/11/21/medline PY - 2017/7/21/entrez KW - electrochemical sensing KW - freestanding paper electrode KW - gold nanoflower KW - real-time monitoring live cells KW - three-dimensional functionalized graphene framework SP - 38201 EP - 38210 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 9 IS - 44 N2 - In this work, we develop a new type of freestanding nanohybrid paper electrode assembled from 3D ionic liquid (IL) functionalized graphene framework (GF) decorated by gold nanoflowers (AuNFs), and explore its practical application in in situ electrochemical sensing of live breast cell samples by real-time tracking biomarker H2O2 released from cells. The AuNFs modified IL functionalized GF (AuNFs/IL-GF) was synthesized via a facile and efficient dopamine-assisted one-pot self-assembly strategy. The as-obtained nanohybrid assembly exhibits a typical 3D hierarchical porous structure, where the highly active electrocatalyst AuNFs are well dispersed on IL-GF scaffold. And the graft of hydrophilic IL molecules (i.e., 1-butyl-3-methylimidazolium tetrafluoroborate, BMIMBF4) on graphene nanosheets not only avoids their agglomeration and disorder stacking during the self-assembly but also endows the integrated IL-GF monolithic material with unique hydrophilic properties, which enables it to be readily dispersed in aqueous solution and processed into freestanding paperlike material. Because of the unique structural properties and the combinational advantages of different components in the AuNFs/IL-GF composite, the resultant nanohybrid paper electrode exhibits good nonenzymatic electrochemical sensing performance toward H2O2. When used in real-time tracking H2O2 secreted from different breast cells attached to the paper electrode without or with radiotherapy treatment, the proposed electrochemical sensor based on freestanding AuNFs/IL-GF paper electrode can distinguish the normal breast cell HBL-100 from the cancer breast cells MDA-MB-231 and MCF-7 cells, and assess the radiotherapy effects to different breast cancer cells, which opens a new horizon in real-time monitoring cancer cells by electrochemical sensing platform. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/28727416/In_Situ_Electrochemical_Sensing_and_Real_Time_Monitoring_Live_Cells_Based_on_Freestanding_Nanohybrid_Paper_Electrode_Assembled_from_3D_Functionalized_Graphene_Framework_ L2 - https://dx.doi.org/10.1021/acsami.7b08781 DB - PRIME DP - Unbound Medicine ER -