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Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water.
Water Res. 2017 10 15; 123:734-750.WR

Abstract

Surface waters can contain a diverse range of organic pollutants, including pesticides, pharmaceuticals and industrial compounds. While bioassays have been used for water quality monitoring, there is limited knowledge regarding the effects of individual micropollutants and their relationship to the overall mixture effect in water samples. In this study, a battery of in vitro bioassays based on human and fish cell lines and whole organism assays using bacteria, algae, daphnids and fish embryos was assembled for use in water quality monitoring. The selection of bioassays was guided by the principles of adverse outcome pathways in order to cover relevant steps in toxicity pathways known to be triggered by environmental water samples. The effects of 34 water pollutants, which were selected based on hazard quotients, available environmental quality standards and mode of action information, were fingerprinted in the bioassay test battery. There was a relatively good agreement between the experimental results and available literature effect data. The majority of the chemicals were active in the assays indicative of apical effects, while fewer chemicals had a response in the specific reporter gene assays, but these effects were typically triggered at lower concentrations. The single chemical effect data were used to improve published mixture toxicity modeling of water samples from the Danube River. While there was a slight increase in the fraction of the bioanalytical equivalents explained for the Danube River samples, for some endpoints less than 1% of the observed effect could be explained by the studied chemicals. The new mixture models essentially confirmed previous findings from many studies monitoring water quality using both chemical analysis and bioanalytical tools. In short, our results indicate that many more chemicals contribute to the biological effect than those that are typically quantified by chemical monitoring programs or those regulated by environmental quality standards. This study not only demonstrates the utility of fingerprinting single chemicals for an improved understanding of the biological effect of pollutants, but also highlights the need to apply bioassays for water quality monitoring in order to prevent underestimation of the overall biological effect.

Authors+Show Affiliations

Australian Rivers Institute, Griffith School of Environment, Griffith University, Southport, QLD, 4222, Australia; The University of Queensland, National Research Centre for Environmental Toxicology (Entox), Brisbane, QLD, 4108, Australia.UFZ - Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.Institut National de l'Environnement Industriel et des Risques INERIS, Unité d'Ecotoxicologie, 60550, Verneuil-en-Halatte, France.Institut National de l'Environnement Industriel et des Risques INERIS, Unité d'Ecotoxicologie, 60550, Verneuil-en-Halatte, France.UFZ - Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.School of Technology, University of Campinas, Limeira, SP, 13484-332, Brazil.Department of Environmental Toxicology, University of California, Davis, CA, 95616, United States.WatchFrog, Bâtiment Genavenir 3, 1 rue Pierre Fontaine, 91000 Evry, France.Masaryk University, Research Centre for Toxic Compounds in the Environment (RECETOX), Kamenice 753/5, 62500 Brno, Czech Republic.Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, 52074 Aachen, Germany.School of Technology, University of Campinas, Limeira, SP, 13484-332, Brazil.Masaryk University, Research Centre for Toxic Compounds in the Environment (RECETOX), Kamenice 753/5, 62500 Brno, Czech Republic.UFZ - Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany.Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, 52074 Aachen, Germany.Institut National de l'Environnement Industriel et des Risques INERIS, Unité d'Ecotoxicologie, 60550, Verneuil-en-Halatte, France.Department of Ecosystem Analysis, Institute for Environmental Research, RWTH Aachen University, 52074 Aachen, Germany.WatchFrog, Bâtiment Genavenir 3, 1 rue Pierre Fontaine, 91000 Evry, France.Norwegian Institute for Water Research NIVA, Gaustadalléen 21, 0349 Oslo, Norway.School of Biosciences, The University of Birmingham, Birmingham, B15 2TT, UK.The University of Queensland, National Research Centre for Environmental Toxicology (Entox), Brisbane, QLD, 4108, Australia; UFZ - Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany; Eberhard Karls University Tübingen, Environmental Toxicology, Center for Applied Geosciences, 72074 Tübingen, Germany. Electronic address: beate.escher@ufz.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28728110

Citation

Neale, Peta A., et al. "Development of a Bioanalytical Test Battery for Water Quality Monitoring: Fingerprinting Identified Micropollutants and Their Contribution to Effects in Surface Water." Water Research, vol. 123, 2017, pp. 734-750.
Neale PA, Altenburger R, Aït-Aïssa S, et al. Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water. Water Res. 2017;123:734-750.
Neale, P. A., Altenburger, R., Aït-Aïssa, S., Brion, F., Busch, W., de Aragão Umbuzeiro, G., Denison, M. S., Du Pasquier, D., Hilscherová, K., Hollert, H., Morales, D. A., Novák, J., Schlichting, R., Seiler, T. B., Serra, H., Shao, Y., Tindall, A. J., Tollefsen, K. E., Williams, T. D., & Escher, B. I. (2017). Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water. Water Research, 123, 734-750. https://doi.org/10.1016/j.watres.2017.07.016
Neale PA, et al. Development of a Bioanalytical Test Battery for Water Quality Monitoring: Fingerprinting Identified Micropollutants and Their Contribution to Effects in Surface Water. Water Res. 2017 10 15;123:734-750. PubMed PMID: 28728110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a bioanalytical test battery for water quality monitoring: Fingerprinting identified micropollutants and their contribution to effects in surface water. AU - Neale,Peta A, AU - Altenburger,Rolf, AU - Aït-Aïssa,Selim, AU - Brion,François, AU - Busch,Wibke, AU - de Aragão Umbuzeiro,Gisela, AU - Denison,Michael S, AU - Du Pasquier,David, AU - Hilscherová,Klára, AU - Hollert,Henner, AU - Morales,Daniel A, AU - Novák,Jiří, AU - Schlichting,Rita, AU - Seiler,Thomas-Benjamin, AU - Serra,Helene, AU - Shao,Ying, AU - Tindall,Andrew J, AU - Tollefsen,Knut Erik, AU - Williams,Timothy D, AU - Escher,Beate I, Y1 - 2017/07/09/ PY - 2017/03/02/received PY - 2017/06/04/revised PY - 2017/07/07/accepted PY - 2017/7/21/pubmed PY - 2017/10/20/medline PY - 2017/7/21/entrez KW - Cell-based bioassay KW - Fish embryo toxicity test KW - In vitro KW - In vivo KW - Mixture toxicity KW - ToxCast SP - 734 EP - 750 JF - Water research JO - Water Res. VL - 123 N2 - Surface waters can contain a diverse range of organic pollutants, including pesticides, pharmaceuticals and industrial compounds. While bioassays have been used for water quality monitoring, there is limited knowledge regarding the effects of individual micropollutants and their relationship to the overall mixture effect in water samples. In this study, a battery of in vitro bioassays based on human and fish cell lines and whole organism assays using bacteria, algae, daphnids and fish embryos was assembled for use in water quality monitoring. The selection of bioassays was guided by the principles of adverse outcome pathways in order to cover relevant steps in toxicity pathways known to be triggered by environmental water samples. The effects of 34 water pollutants, which were selected based on hazard quotients, available environmental quality standards and mode of action information, were fingerprinted in the bioassay test battery. There was a relatively good agreement between the experimental results and available literature effect data. The majority of the chemicals were active in the assays indicative of apical effects, while fewer chemicals had a response in the specific reporter gene assays, but these effects were typically triggered at lower concentrations. The single chemical effect data were used to improve published mixture toxicity modeling of water samples from the Danube River. While there was a slight increase in the fraction of the bioanalytical equivalents explained for the Danube River samples, for some endpoints less than 1% of the observed effect could be explained by the studied chemicals. The new mixture models essentially confirmed previous findings from many studies monitoring water quality using both chemical analysis and bioanalytical tools. In short, our results indicate that many more chemicals contribute to the biological effect than those that are typically quantified by chemical monitoring programs or those regulated by environmental quality standards. This study not only demonstrates the utility of fingerprinting single chemicals for an improved understanding of the biological effect of pollutants, but also highlights the need to apply bioassays for water quality monitoring in order to prevent underestimation of the overall biological effect. SN - 1879-2448 UR - https://www.unboundmedicine.com/medline/citation/28728110/Development_of_a_bioanalytical_test_battery_for_water_quality_monitoring:_Fingerprinting_identified_micropollutants_and_their_contribution_to_effects_in_surface_water_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0043-1354(17)30589-4 DB - PRIME DP - Unbound Medicine ER -