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Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh.
BMC Res Notes. 2017 Jul 20; 10(1):299.BR

Abstract

BACKGROUND

Melioidosis an infectious disease, caused by a Gram negative bacterium called Burkholderia pseudomallei, is endemic in Bangladesh. This organism is sensitive to limited number of antimicrobial agents and need prolonged treatment. There is no comprehensive data on the antimicrobial susceptibility profile of B. pseudomallei isolated in Bangladesh over last several years. The present study aimed to determine the antimicrobial susceptibility pattern of B. pseudomallei isolated in a tertiary care hospital of Dhaka city from 2009 to 2015.

METHODS

All B. pseudomallei isolated from melioidosis patients over a period of 7 years (2009-2015) in the Department of Microbiology of a 725-bed tertiary care referral hospital in Dhaka city, Bangladesh were included in the study. B. pseudomallei was identified by Gram stain, culture, specific biochemical tests, serology and PCR using specific primers constructed from 16s rRNA region of B. pseudomallei. Antimicrobial susceptibility to specific agents was determined by disk diffusion and minimum inhibitory concentration methods.

RESULTS

A total of 20 isolates of B. pseudomallei which were isolated from patients coming from different geographic locations of Bangladesh were included in the study. All the isolates were uniformly sensitive (100%) to ceftazidime, imipenem, piperacillin-tazobactam, amoxicillin-clavulanic acid and tetracycline by both disk diffusion and MIC methods. Two strains were resistant to trimethoprim-sulfamethoxazole by disk diffusion method but were sensitive by MIC method. The MIC50 and MIC90 values of the above antimicrobial agents were almost similar. All the isolates were resistant to amikacin by both MIC and disk diffusion methods.

CONCLUSION

The results of the study suggest that B. pseudomallei prevalent in Bangladesh were still susceptible to all recommended antimicrobial agents used for the treatment of melioidosis. However, regular monitoring is needed to detect any emergence of resistance and shifting of MIC50 and MIC90 values.

Authors+Show Affiliations

Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh.Department of Microbiology, Ibrahim Medical College, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh.Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh.Department of Microbiology, Ibrahim Medical College, 122 Kazi Nazrul Islam Avenue, Dhaka, 1000, Bangladesh. jahaq54@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28728591

Citation

Dutta, Subarna, et al. "Antimicrobial Susceptibility Pattern of Clinical Isolates of Burkholderia Pseudomallei in Bangladesh." BMC Research Notes, vol. 10, no. 1, 2017, p. 299.
Dutta S, Haq S, Hasan MR, et al. Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh. BMC Res Notes. 2017;10(1):299.
Dutta, S., Haq, S., Hasan, M. R., & Haq, J. A. (2017). Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh. BMC Research Notes, 10(1), 299. https://doi.org/10.1186/s13104-017-2626-5
Dutta S, et al. Antimicrobial Susceptibility Pattern of Clinical Isolates of Burkholderia Pseudomallei in Bangladesh. BMC Res Notes. 2017 Jul 20;10(1):299. PubMed PMID: 28728591.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial susceptibility pattern of clinical isolates of Burkholderia pseudomallei in Bangladesh. AU - Dutta,Subarna, AU - Haq,Sabah, AU - Hasan,Mohammad Rokibul, AU - Haq,Jalaluddin Ashraful, Y1 - 2017/07/20/ PY - 2016/11/01/received PY - 2017/07/13/accepted PY - 2017/7/22/entrez PY - 2017/7/22/pubmed PY - 2018/4/17/medline KW - Antimicrobial susceptibility KW - Bangladesh KW - Burkholderia pseudomallei KW - Minimum inhibitory concentration SP - 299 EP - 299 JF - BMC research notes JO - BMC Res Notes VL - 10 IS - 1 N2 - BACKGROUND: Melioidosis an infectious disease, caused by a Gram negative bacterium called Burkholderia pseudomallei, is endemic in Bangladesh. This organism is sensitive to limited number of antimicrobial agents and need prolonged treatment. There is no comprehensive data on the antimicrobial susceptibility profile of B. pseudomallei isolated in Bangladesh over last several years. The present study aimed to determine the antimicrobial susceptibility pattern of B. pseudomallei isolated in a tertiary care hospital of Dhaka city from 2009 to 2015. METHODS: All B. pseudomallei isolated from melioidosis patients over a period of 7 years (2009-2015) in the Department of Microbiology of a 725-bed tertiary care referral hospital in Dhaka city, Bangladesh were included in the study. B. pseudomallei was identified by Gram stain, culture, specific biochemical tests, serology and PCR using specific primers constructed from 16s rRNA region of B. pseudomallei. Antimicrobial susceptibility to specific agents was determined by disk diffusion and minimum inhibitory concentration methods. RESULTS: A total of 20 isolates of B. pseudomallei which were isolated from patients coming from different geographic locations of Bangladesh were included in the study. All the isolates were uniformly sensitive (100%) to ceftazidime, imipenem, piperacillin-tazobactam, amoxicillin-clavulanic acid and tetracycline by both disk diffusion and MIC methods. Two strains were resistant to trimethoprim-sulfamethoxazole by disk diffusion method but were sensitive by MIC method. The MIC50 and MIC90 values of the above antimicrobial agents were almost similar. All the isolates were resistant to amikacin by both MIC and disk diffusion methods. CONCLUSION: The results of the study suggest that B. pseudomallei prevalent in Bangladesh were still susceptible to all recommended antimicrobial agents used for the treatment of melioidosis. However, regular monitoring is needed to detect any emergence of resistance and shifting of MIC50 and MIC90 values. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/28728591/Antimicrobial_susceptibility_pattern_of_clinical_isolates_of_Burkholderia_pseudomallei_in_Bangladesh_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2626-5 DB - PRIME DP - Unbound Medicine ER -