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Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats.
Andrologia. 2018 Mar; 50(2)A

Abstract

The study was designed to evaluate the effects of metformin on apoptosis and epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury model. A total of 72 male rats were divided into four groups (n = 18 for each group): group 1 (sham-operated group), group 2 (metformin group), group 3 (torsion/detorsion [T/D] + saline) and group 4 (T/D + 300 mg kg-1 metformin). Testicular torsion was achieved by rotating the right testis 720° in a clockwise direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in comparison with sham-operated group 4 hr after detorsion (p < .001). In six rats of each group 24 hr after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubule diameters (MSTD) and TUNEL test. Moreover, 30 days after T/D, sperm concentration and motility were examined in six animals per group. Metformin pre-treatment reduced MDA and caspase-3 levels and normalised antioxidant enzyme activities 4 hr after detorsion, and germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, was significantly improved. Reduction in oxidative stress and apoptosis may have a major role in cytoprotective effects of metformin.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran.Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy and Reproductive Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Department of Pathology, Urmia University of Medical Science, Urmia, Iran.Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28730645

Citation

Ghasemnejad-Berenji, M, et al. "Effect of Metformin On Germ Cell-specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/detorsion in Rats." Andrologia, vol. 50, no. 2, 2018.
Ghasemnejad-Berenji M, Ghazi-Khansari M, Yazdani I, et al. Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats. Andrologia. 2018;50(2).
Ghasemnejad-Berenji, M., Ghazi-Khansari, M., Yazdani, I., Nobakht, M., Abdollahi, A., Ghasemnejad-Berenji, H., Mohajer Ansari, J., Pashapour, S., & Dehpour, A. R. (2018). Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats. Andrologia, 50(2). https://doi.org/10.1111/and.12846
Ghasemnejad-Berenji M, et al. Effect of Metformin On Germ Cell-specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/detorsion in Rats. Andrologia. 2018;50(2) PubMed PMID: 28730645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of metformin on germ cell-specific apoptosis, oxidative stress and epididymal sperm quality after testicular torsion/detorsion in rats. AU - Ghasemnejad-Berenji,M, AU - Ghazi-Khansari,M, AU - Yazdani,I, AU - Nobakht,M, AU - Abdollahi,A, AU - Ghasemnejad-Berenji,H, AU - Mohajer Ansari,J, AU - Pashapour,S, AU - Dehpour,A R, Y1 - 2017/07/21/ PY - 2017/03/21/accepted PY - 2017/7/22/pubmed PY - 2018/9/19/medline PY - 2017/7/22/entrez KW - apoptosis KW - metformin KW - oxidative stress KW - testicular torsion/detorsion JF - Andrologia JO - Andrologia VL - 50 IS - 2 N2 - The study was designed to evaluate the effects of metformin on apoptosis and epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury model. A total of 72 male rats were divided into four groups (n = 18 for each group): group 1 (sham-operated group), group 2 (metformin group), group 3 (torsion/detorsion [T/D] + saline) and group 4 (T/D + 300 mg kg-1 metformin). Testicular torsion was achieved by rotating the right testis 720° in a clockwise direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in comparison with sham-operated group 4 hr after detorsion (p < .001). In six rats of each group 24 hr after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubule diameters (MSTD) and TUNEL test. Moreover, 30 days after T/D, sperm concentration and motility were examined in six animals per group. Metformin pre-treatment reduced MDA and caspase-3 levels and normalised antioxidant enzyme activities 4 hr after detorsion, and germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, was significantly improved. Reduction in oxidative stress and apoptosis may have a major role in cytoprotective effects of metformin. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/28730645/Effect_of_metformin_on_germ_cell_specific_apoptosis_oxidative_stress_and_epididymal_sperm_quality_after_testicular_torsion/detorsion_in_rats_ L2 - https://doi.org/10.1111/and.12846 DB - PRIME DP - Unbound Medicine ER -