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Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties.
Int J Biol Macromol. 2017 Dec; 105(Pt 2):1519-1525.IJ

Abstract

This study investigated the antioxidant and antibacterial properties of crude water-soluble polysaccharides extracted from the mallow (Malva aegyptiaca) by precipitation with cetylpyridinium chloride (P1) or ethanol (P2). The Polysaccharides fractions were characterized by FTIR spectroscopy, a monosaccharide composition, and antioxidant and antibacterial activities. P1 showed the highest total sugars (81.2%) and sulfated groups (2.9%) contents but with the lowest total proteins content (8.7%). The infrared spectroscopic spectra exhibited the typical bands and peak characteristic of polysaccharides. Monosaccharides analysis revealed the dominance of galactose (40.3-43.7%) and glucuronic acid (25.9-30.9%). Interestingly, P1 displayed an important antioxidant activity as evaluated by the (Fe2+) chelating activity (IC50=1.15mg/ml), (Fe3+) reducing power (EC50=1.22mg/ml), β-carotene bleaching inhibition capacity (IC50=1.56mg/ml) and DPPH-radical scavenging activity (IC50=1.94mg/ml). Furthermore, P1 at 10mg/ml was highly active than P2 against several bacterial strains and especially the Gram-positive bacteria, where the inhibition rate ranged between 84.2 and 90.3%. Obtained results suggest that M. aegyptiaca polysaccharides precipitated by cetylpyridinium chloride could be used as a naural antioxidant and antibacterial agent.

Authors+Show Affiliations

Laboratory of Enzyme Engineering and Microbiology, Engineering National School of Sfax (ENIS), University of Sfax, Sfax, Tunisia.Laboratory of Enzyme Engineering and Microbiology, Engineering National School of Sfax (ENIS), University of Sfax, Sfax, Tunisia.Laboratory of Enzyme Engineering of Lipases and Biochemistry, ENIS, University of Sfax, Sfax, Tunisia.Laboratory of Enzyme Engineering and Microbiology, Engineering National School of Sfax (ENIS), University of Sfax, Sfax, Tunisia.High Institute of Applied Biology of Medenine (ISBAM), University of Gabes, Medenine, Tunisia. Electronic address: znacim2002@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28732725

Citation

Fakhfakh, Nahed, et al. "Isolation of Polysaccharides From Malva Aegyptiaca and Evaluation of Their Antioxidant and Antibacterial Properties." International Journal of Biological Macromolecules, vol. 105, no. Pt 2, 2017, pp. 1519-1525.
Fakhfakh N, Abdelhedi O, Jdir H, et al. Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties. Int J Biol Macromol. 2017;105(Pt 2):1519-1525.
Fakhfakh, N., Abdelhedi, O., Jdir, H., Nasri, M., & Zouari, N. (2017). Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties. International Journal of Biological Macromolecules, 105(Pt 2), 1519-1525. https://doi.org/10.1016/j.ijbiomac.2017.07.105
Fakhfakh N, et al. Isolation of Polysaccharides From Malva Aegyptiaca and Evaluation of Their Antioxidant and Antibacterial Properties. Int J Biol Macromol. 2017;105(Pt 2):1519-1525. PubMed PMID: 28732725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties. AU - Fakhfakh,Nahed, AU - Abdelhedi,Ola, AU - Jdir,Hamida, AU - Nasri,Moncef, AU - Zouari,Nacim, Y1 - 2017/07/18/ PY - 2017/06/08/received PY - 2017/07/04/revised PY - 2017/07/16/accepted PY - 2017/7/25/pubmed PY - 2018/6/5/medline PY - 2017/7/23/entrez KW - Antibacterial KW - Antioxidant KW - Cetylpyridinium chloride KW - Malva aegyptiaca KW - Monosaccharide composition KW - Polysaccharides SP - 1519 EP - 1525 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 105 IS - Pt 2 N2 - This study investigated the antioxidant and antibacterial properties of crude water-soluble polysaccharides extracted from the mallow (Malva aegyptiaca) by precipitation with cetylpyridinium chloride (P1) or ethanol (P2). The Polysaccharides fractions were characterized by FTIR spectroscopy, a monosaccharide composition, and antioxidant and antibacterial activities. P1 showed the highest total sugars (81.2%) and sulfated groups (2.9%) contents but with the lowest total proteins content (8.7%). The infrared spectroscopic spectra exhibited the typical bands and peak characteristic of polysaccharides. Monosaccharides analysis revealed the dominance of galactose (40.3-43.7%) and glucuronic acid (25.9-30.9%). Interestingly, P1 displayed an important antioxidant activity as evaluated by the (Fe2+) chelating activity (IC50=1.15mg/ml), (Fe3+) reducing power (EC50=1.22mg/ml), β-carotene bleaching inhibition capacity (IC50=1.56mg/ml) and DPPH-radical scavenging activity (IC50=1.94mg/ml). Furthermore, P1 at 10mg/ml was highly active than P2 against several bacterial strains and especially the Gram-positive bacteria, where the inhibition rate ranged between 84.2 and 90.3%. Obtained results suggest that M. aegyptiaca polysaccharides precipitated by cetylpyridinium chloride could be used as a naural antioxidant and antibacterial agent. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28732725/Isolation_of_polysaccharides_from_Malva_aegyptiaca_and_evaluation_of_their_antioxidant_and_antibacterial_properties_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32069-X DB - PRIME DP - Unbound Medicine ER -