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Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography.
Talanta 2017; 174:703-714T

Abstract

A novel multifunctional stationary phase based on silica gel was synthesised starting from L- isoleucine and 4-phenylbutylamine and evaluated as a hydrophilic interaction/reversed-phase mixed-mode stationary phase for high-performance liquid chromatography (HPLC). The prepared stationary phase was characterized by elemental analysis, infrared spectroscopy (IR), scanning electron microscopy (SEM), Brunauer, Emmett and Teller (BET) and solid-state 13C nuclear magnetic resonance (NMR). The mechanisms involved in the chromatographic separation are multi-interaction, including hydrophobic, π-π, hydrogen-bonding, dipole-dipole and ion-dipole interactions. Based on these interactions, successful separation could be achieved among several aromatic compounds having different polarities under both hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) condition. Nucleotides/nucleosides were separated in the HILIC mode. The effects of different separation conditions, such as pH value, mobile-phase content, column temperature, buffer concentration and flow rate, on the separation of nucleotides/nucleosides in HILIC mode were investigated. The seven nucleotides/nucleosides were separated within 22min, while six of them were separated within 10min by isocratic elution. To determine the influence of the new multifunctional stationary phase under the RP condition, a number of moderately and weakly polar and nonpolar compounds, such as 10 substituted anilines and eight substituted phenols were separated successfully under the RP condition within 14 and 15min, respectively. Additionally, nine mixtures of polar/nonpolar test compounds were simultaneously separated within 19min, while seven of them were separated within 12min, under HILIC/RP mixed-mode conditions. Chromatographic parameters, such as the retention factor and peak asymmetry factor, were calculated for all of the analytes, while the theoretical plate number was calculated for analytes separated by isocratic elution. Compared to traditional C18 and commercial HILIC columns, the new stationary phase exhibited both HILIC and RPLC performance, and the scope of analyte separation was thus enlarged.

Authors+Show Affiliations

University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey.University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey.University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey.University of Batman, Faculty of Science and Art, Department of Chemistry, Batman, Turkey. Electronic address: tarik.aral@batman.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28738646

Citation

Aral, Hayriye, et al. "Synthesis, Characterization, and Application of a Novel Multifunctional Stationary Phase for Hydrophilic Interaction/reversed Phase Mixed-mode Chromatography." Talanta, vol. 174, 2017, pp. 703-714.
Aral H, Çelik KS, Altındağ R, et al. Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography. Talanta. 2017;174:703-714.
Aral, H., Çelik, K. S., Altındağ, R., & Aral, T. (2017). Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography. Talanta, 174, pp. 703-714. doi:10.1016/j.talanta.2017.07.014.
Aral H, et al. Synthesis, Characterization, and Application of a Novel Multifunctional Stationary Phase for Hydrophilic Interaction/reversed Phase Mixed-mode Chromatography. Talanta. 2017 Nov 1;174:703-714. PubMed PMID: 28738646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synthesis, characterization, and application of a novel multifunctional stationary phase for hydrophilic interaction/reversed phase mixed-mode chromatography. AU - Aral,Hayriye, AU - Çelik,K Serdar, AU - Altındağ,Ramazan, AU - Aral,Tarık, Y1 - 2017/07/03/ PY - 2017/06/05/received PY - 2017/07/01/revised PY - 2017/07/01/accepted PY - 2017/7/26/entrez PY - 2017/7/26/pubmed PY - 2018/9/4/medline KW - Hydrophilic interaction liquid chromatography (HILIC) KW - Mixed-mode chromatography KW - Mixed-mode stationary phase KW - Reversed phase liquid chromatography SP - 703 EP - 714 JF - Talanta JO - Talanta VL - 174 N2 - A novel multifunctional stationary phase based on silica gel was synthesised starting from L- isoleucine and 4-phenylbutylamine and evaluated as a hydrophilic interaction/reversed-phase mixed-mode stationary phase for high-performance liquid chromatography (HPLC). The prepared stationary phase was characterized by elemental analysis, infrared spectroscopy (IR), scanning electron microscopy (SEM), Brunauer, Emmett and Teller (BET) and solid-state 13C nuclear magnetic resonance (NMR). The mechanisms involved in the chromatographic separation are multi-interaction, including hydrophobic, π-π, hydrogen-bonding, dipole-dipole and ion-dipole interactions. Based on these interactions, successful separation could be achieved among several aromatic compounds having different polarities under both hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) condition. Nucleotides/nucleosides were separated in the HILIC mode. The effects of different separation conditions, such as pH value, mobile-phase content, column temperature, buffer concentration and flow rate, on the separation of nucleotides/nucleosides in HILIC mode were investigated. The seven nucleotides/nucleosides were separated within 22min, while six of them were separated within 10min by isocratic elution. To determine the influence of the new multifunctional stationary phase under the RP condition, a number of moderately and weakly polar and nonpolar compounds, such as 10 substituted anilines and eight substituted phenols were separated successfully under the RP condition within 14 and 15min, respectively. Additionally, nine mixtures of polar/nonpolar test compounds were simultaneously separated within 19min, while seven of them were separated within 12min, under HILIC/RP mixed-mode conditions. Chromatographic parameters, such as the retention factor and peak asymmetry factor, were calculated for all of the analytes, while the theoretical plate number was calculated for analytes separated by isocratic elution. Compared to traditional C18 and commercial HILIC columns, the new stationary phase exhibited both HILIC and RPLC performance, and the scope of analyte separation was thus enlarged. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/28738646/Synthesis_characterization_and_application_of_a_novel_multifunctional_stationary_phase_for_hydrophilic_interaction/reversed_phase_mixed_mode_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(17)30726-9 DB - PRIME DP - Unbound Medicine ER -