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Transcriptome changes associated with Tomato spotted wilt virus infection in various life stages of its thrips vector, Frankliniella fusca (Hinds).
J Gen Virol. 2017 Aug; 98(8):2156-2170.JG

Abstract

Persistent propagative viruses maintain intricate interactions with their arthropod vectors. In this study, we investigated the transcriptome-level responses associated with a persistent propagative phytovirus infection in various life stages of its vector using an Illumina HiSeq sequencing platform. The pathosystem components included a Tospovirus, Tomato spotted wilt virus (TSWV), its insect vector, Frankliniella fusca (Hinds), and a plant host, Arachis hypogaea (L.). We assembled (de novo) reads from three developmental stage groups of virus-exposed and non-virus-exposed F. fusca into one transcriptome consisting of 72 366 contigs and identified 1161 differentially expressed (DE) contigs. The number of DE contigs was greatest in adults (female) (562) when compared with larvae (first and second instars) (395) and pupae (pre- and pupae) (204). Upregulated contigs in virus-exposed thrips had blastx annotations associated with intracellular transport and virus replication. Upregulated contigs were also assigned blastx annotations associated with immune responses, including apoptosis and phagocytosis. In virus-exposed larvae, Blast2GO analysis identified functional groups, such as multicellular development with downregulated contigs, while reproduction, embryo development and growth were identified with upregulated contigs in virus-exposed adults. This study provides insights into differences in transcriptome-level responses modulated by TSWV in various life stages of an important vector, F. fusca.

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Authors+Show Affiliations

Shrestha A
Department of Entomology, University of Georgia, Tifton, GA 31793, USA.
Champagne DE
Department of Entomology, University of Georgia, Tifton, GA 31793, USA.
Culbreath AK
Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA.
Rotenberg D
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Whitfield AE
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Srinivasan R
Department of Entomology, University of Georgia, Tifton, GA 31793, USA.

MeSH

AnimalsInsect ProteinsInsect VectorsLarvaPlant DiseasesThysanopteraTospovirusTranscriptome

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28741996

Citation

Shrestha, Anita, et al. "Transcriptome Changes Associated With Tomato Spotted Wilt Virus Infection in Various Life Stages of Its Thrips Vector, Frankliniella Fusca (Hinds)." The Journal of General Virology, vol. 98, no. 8, 2017, pp. 2156-2170.
Shrestha A, Champagne DE, Culbreath AK, et al. Transcriptome changes associated with Tomato spotted wilt virus infection in various life stages of its thrips vector, Frankliniella fusca (Hinds). J Gen Virol. 2017;98(8):2156-2170.
Shrestha, A., Champagne, D. E., Culbreath, A. K., Rotenberg, D., Whitfield, A. E., & Srinivasan, R. (2017). Transcriptome changes associated with Tomato spotted wilt virus infection in various life stages of its thrips vector, Frankliniella fusca (Hinds). The Journal of General Virology, 98(8), 2156-2170. https://doi.org/10.1099/jgv.0.000874
Shrestha A, et al. Transcriptome Changes Associated With Tomato Spotted Wilt Virus Infection in Various Life Stages of Its Thrips Vector, Frankliniella Fusca (Hinds). J Gen Virol. 2017;98(8):2156-2170. PubMed PMID: 28741996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome changes associated with Tomato spotted wilt virus infection in various life stages of its thrips vector, Frankliniella fusca (Hinds). AU - Shrestha,Anita, AU - Champagne,Donald E, AU - Culbreath,Albert K, AU - Rotenberg,Dorith, AU - Whitfield,Anna E, AU - Srinivasan,Rajagopalbabu, Y1 - 2017/07/26/ PY - 2017/7/26/pubmed PY - 2017/9/7/medline PY - 2017/7/26/entrez SP - 2156 EP - 2170 JF - The Journal of general virology JO - J Gen Virol VL - 98 IS - 8 N2 - Persistent propagative viruses maintain intricate interactions with their arthropod vectors. In this study, we investigated the transcriptome-level responses associated with a persistent propagative phytovirus infection in various life stages of its vector using an Illumina HiSeq sequencing platform. The pathosystem components included a Tospovirus, Tomato spotted wilt virus (TSWV), its insect vector, Frankliniella fusca (Hinds), and a plant host, Arachis hypogaea (L.). We assembled (de novo) reads from three developmental stage groups of virus-exposed and non-virus-exposed F. fusca into one transcriptome consisting of 72 366 contigs and identified 1161 differentially expressed (DE) contigs. The number of DE contigs was greatest in adults (female) (562) when compared with larvae (first and second instars) (395) and pupae (pre- and pupae) (204). Upregulated contigs in virus-exposed thrips had blastx annotations associated with intracellular transport and virus replication. Upregulated contigs were also assigned blastx annotations associated with immune responses, including apoptosis and phagocytosis. In virus-exposed larvae, Blast2GO analysis identified functional groups, such as multicellular development with downregulated contigs, while reproduction, embryo development and growth were identified with upregulated contigs in virus-exposed adults. This study provides insights into differences in transcriptome-level responses modulated by TSWV in various life stages of an important vector, F. fusca. SN - 1465-2099 UR - https://www.unboundmedicine.com/medline/citation/28741996/Transcriptome_changes_associated_with_Tomato_spotted_wilt_virus_infection_in_various_life_stages_of_its_thrips_vector_Frankliniella_fusca__Hinds__ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/jgv.0.000874 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓19]

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