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Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia.
Int J Reprod Biomed. 2017 May; 15(5):279-286.IJ

Abstract

BACKGROUND

Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology.

OBJECTIVE

To compare the level of intracellular anion superoxide (O2-), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men.

MATERIALS AND METHODS

In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40%) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2- and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test.

RESULTS

The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2- levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2- and HSPA2 in the case group (r=0.041, p=0.79).

CONCLUSION

We suggest that the increase in intracellular O2-, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients.

Authors+Show Affiliations

Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.Department of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28744523

Citation

Sabeti, Parvin, et al. "Evaluation of Intracellular Anion Superoxide Level, Heat Shock Protein A2 and Protamine Positive Spermatozoa Percentages in Teratoasthenozoospermia." International Journal of Reproductive Biomedicine, vol. 15, no. 5, 2017, pp. 279-286.
Sabeti P, Amidi F, Kalantar SM, et al. Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia. Int J Reprod Biomed. 2017;15(5):279-286.
Sabeti, P., Amidi, F., Kalantar, S. M., Sedighi Gilani, M. A., Pourmasumi, S., Najafi, A., & Talebi, A. R. (2017). Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia. International Journal of Reproductive Biomedicine, 15(5), 279-286.
Sabeti P, et al. Evaluation of Intracellular Anion Superoxide Level, Heat Shock Protein A2 and Protamine Positive Spermatozoa Percentages in Teratoasthenozoospermia. Int J Reprod Biomed. 2017;15(5):279-286. PubMed PMID: 28744523.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia. AU - Sabeti,Parvin, AU - Amidi,Fardin, AU - Kalantar,Seyed Mahdi, AU - Sedighi Gilani,Mohammad Ali, AU - Pourmasumi,Soheila, AU - Najafi,Atefeh, AU - Talebi,Ali Reza, PY - 2017/7/27/entrez PY - 2017/7/27/pubmed PY - 2017/7/27/medline KW - HSPA2 KW - Male infertility KW - Protamine deficiency KW - Sperm chromatin SP - 279 EP - 286 JF - International journal of reproductive biomedicine JO - Int J Reprod Biomed VL - 15 IS - 5 N2 - BACKGROUND: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology. OBJECTIVE: To compare the level of intracellular anion superoxide (O2-), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men. MATERIALS AND METHODS: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40%) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2- and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test. RESULTS: The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2- levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2- and HSPA2 in the case group (r=0.041, p=0.79). CONCLUSION: We suggest that the increase in intracellular O2-, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients. SN - 2476-4108 UR - https://www.unboundmedicine.com/medline/citation/28744523/Evaluation_of_intracellular_anion_superoxide_level_heat_shock_protein_A2_and_protamine_positive_spermatozoa_percentages_in_teratoasthenozoospermia_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28744523/ DB - PRIME DP - Unbound Medicine ER -
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