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Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities.
An Acad Bras Cienc. 2017; 89(3 Suppl):2247-2259.AA

Abstract

Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS·+, ·DPPH, and β-carotene/linoleic acid co-oxidation methods. The antibacterial activity was determined using a microdilution method with Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus. The antiproliferative effect was determined in tumor cell lines using the Alamar Blue assay. The chemical profile was obtained using UHPLC-MS and UHPLC-MS/MS. The butanolic fraction had the highest concentration of phenolic compounds and more potent antioxidant properties in all assays. This fraction also had bacteriostatic and bactericidal effects against all bacterial strains at low concentrations, especially S. aureus. The hexane fraction had the highest antiproliferative potential, with IC50 values ranging from 24.2 to 46.6 µg/mL in HL-60 (human promyelocytic leukemia cell) and K562 (human chronic myelocytic leukemia cell), respectively. Preliminary chemical analysis indicates the presence of terpenes and gallic acid in the geopropolis. Our results indicate the therapeutic potential of geopropolis from M. mondury against inflammatory, oxidative, infectious, and neoplastic diseases.

Authors+Show Affiliations

Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Jequiezinho, 45208-091 Jequié, BA, Brazil.Departamento de Ciências Naturais, Universidade Estadual do Sudoeste da Bahia, Estrada do Bem Querer, Km 04, s/n, 45031-900 Vitória da Conquista, BA, Brazil.Departamento de Biologia e Ciências Farmacêuticas, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, Cidade Universitária, 13083-862 Campinas, SP, Brazil.Departamento de Biologia e Ciências Farmacêuticas, Instituto de Biologia, Universidade Estadual de Campinas, Rua Monteiro Lobato, 255, Cidade Universitária, 13083-862 Campinas, SP, Brazil.Instituto Gonçalo Moniz, Fundação Oswaldo Cruz/IGM-FIOCRUZ-BA, Rua Waldemar Falcão, 121, Candeal, 40296-710 Salvador, BA, Brazil. Centro de Biotecnologia e Terapia Celular, Hospital São Rafael, Avenida São Rafael, 2152, São Marcos, 41253-190 Salvador, BA, Brazil.Instituto Gonçalo Moniz, Fundação Oswaldo Cruz/IGM-FIOCRUZ-BA, Rua Waldemar Falcão, 121, Candeal, 40296-710 Salvador, BA, Brazil.Instituto Gonçalo Moniz, Fundação Oswaldo Cruz/IGM-FIOCRUZ-BA, Rua Waldemar Falcão, 121, Candeal, 40296-710 Salvador, BA, Brazil.Departmento de Química e Ciências Exatas, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Jequiezinho, 45208-091 Jequié, BA, Brazil.Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Jequiezinho, 45208-091 Jequié, BA, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28746550

Citation

Santos, Tássia L A Dos, et al. "Melipona Mondury Produces a Geopropolis With Antioxidant, Antibacterial and Antiproliferative Activities." Anais Da Academia Brasileira De Ciencias, vol. 89, no. 3 Suppl, 2017, pp. 2247-2259.
Santos TLAD, Queiroz RF, Sawaya ACHF, et al. Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities. An Acad Bras Cienc. 2017;89(3 Suppl):2247-2259.
Santos, T. L. A. D., Queiroz, R. F., Sawaya, A. C. H. F., Lopez, B. G., Soares, M. B. P., Bezerra, D. P., Rodrigues, A. C. B. C., Paula, V. F., & Waldschmidt, A. M. (2017). Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities. Anais Da Academia Brasileira De Ciencias, 89(3 Suppl), 2247-2259. https://doi.org/10.1590/0001-3765201720160725
Santos TLAD, et al. Melipona Mondury Produces a Geopropolis With Antioxidant, Antibacterial and Antiproliferative Activities. An Acad Bras Cienc. 2017;89(3 Suppl):2247-2259. PubMed PMID: 28746550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities. AU - Santos,Tássia L A Dos, AU - Queiroz,Raphael F, AU - Sawaya,Alexandra C H F, AU - Lopez,Begoña Gimenez-Cassina, AU - Soares,Milena B P, AU - Bezerra,Daniel P, AU - Rodrigues,Ana Carolina B C, AU - Paula,Vanderlúcia F DE, AU - Waldschmidt,Ana Maria, Y1 - 2017/07/20/ PY - 2016/10/26/received PY - 2017/03/03/accepted PY - 2017/7/27/pubmed PY - 2018/5/31/medline PY - 2017/7/27/entrez SP - 2247 EP - 2259 JF - Anais da Academia Brasileira de Ciencias JO - An. Acad. Bras. Cienc. VL - 89 IS - 3 Suppl N2 - Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS·+, ·DPPH, and β-carotene/linoleic acid co-oxidation methods. The antibacterial activity was determined using a microdilution method with Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus. The antiproliferative effect was determined in tumor cell lines using the Alamar Blue assay. The chemical profile was obtained using UHPLC-MS and UHPLC-MS/MS. The butanolic fraction had the highest concentration of phenolic compounds and more potent antioxidant properties in all assays. This fraction also had bacteriostatic and bactericidal effects against all bacterial strains at low concentrations, especially S. aureus. The hexane fraction had the highest antiproliferative potential, with IC50 values ranging from 24.2 to 46.6 µg/mL in HL-60 (human promyelocytic leukemia cell) and K562 (human chronic myelocytic leukemia cell), respectively. Preliminary chemical analysis indicates the presence of terpenes and gallic acid in the geopropolis. Our results indicate the therapeutic potential of geopropolis from M. mondury against inflammatory, oxidative, infectious, and neoplastic diseases. SN - 1678-2690 UR - https://www.unboundmedicine.com/medline/citation/28746550/Melipona_mondury_produces_a_geopropolis_with_antioxidant_antibacterial_and_antiproliferative_activities_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005012102&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -