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Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar.
Vet Microbiol. 2017 Aug; 207:69-73.VM

Abstract

Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains are food-borne pathogens associated with acute diarrhea. Haemolytic-uremic syndrome (HUS) is often a complication of STEC infection. In order to examine the occurrence, serotypes, virulence and antimicrobial-resistance profiles of STEC and EPEC in wildlife, 326 faecal E. coli strains from 304 clinically healthy animals were analyzed. For this approach stx1, stx2 and eae genes, as well as accessory virulence determinants (ehx, hlyA, saa, tia, bfp, subAB) were PCR-screened and sequenced. Serotyping was performed employing all available O (O1-O185) and H (H1-H56) antisera. Genetic diversity was analyzed by XbaI-PFGE and phylotyping. Thirteen STEC (4.3%) and 10 EPEC (3.3%) were identified among 12 deer, 3 mouflon, 6 wild boars and 2 birds. Nine STEC showed seropathotypes B (O145:[H28]) and C (O22:H8, O128:[H2]) associated with HUS, and D (O110:H28, O146:H21, O146:[H28], ONT:H8) associated with human diarrhea. Although most isolates harbored stx2b and stx1c variants, stx2a and stx1a (related with severe disease) were also detected. Additionally, the eae gene was present in one stx2a-positive O145:[H28] STEC from a deer and 11 STEC harbored subAB genes (mainly the subAB2 variant). EPEC isolates showed 7 different intimin variants (β1, β2, γ1, ε1, ζ1, ι1-A, κ). Interestingly, the O49:[H10] eae-κ EPEC isolated from a wild boar was bfpA-positive showing a combination of serotype/virulence profile previously detected among human clinical tEPEC. Based on present results, wild ruminants, wild boars and to a lesser extent birds would be carriers of potentially pathogenic STEC and EPEC strains.

Authors+Show Affiliations

Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Universidad de Santiago de Compostela, Lugo, Spain.Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Universidad de Santiago de Compostela, Lugo, Spain.Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Universidad de Santiago de Compostela, Lugo, Spain.Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Grupo SaBio, Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Facultad de Veterinaria,Universidad de Zaragoza, Zaragoza, Spain.Laboratorio de Referencia de E. coli (LREC), Departamento de Microbioloxía e Parasitoloxía, Universidad de Santiago de Compostela, Lugo, Spain.Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain. Electronic address: carmen.torres@unirioja.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28757042

Citation

Alonso, Carla Andrea, et al. "Occurrence and Characterization of Stx And/or Eae-positive Escherichia Coli Isolated From Wildlife, Including a Typical EPEC Strain From a Wild Boar." Veterinary Microbiology, vol. 207, 2017, pp. 69-73.
Alonso CA, Mora A, Díaz D, et al. Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar. Vet Microbiol. 2017;207:69-73.
Alonso, C. A., Mora, A., Díaz, D., Blanco, M., González-Barrio, D., Ruiz-Fons, F., Simón, C., Blanco, J., & Torres, C. (2017). Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar. Veterinary Microbiology, 207, 69-73. https://doi.org/10.1016/j.vetmic.2017.05.028
Alonso CA, et al. Occurrence and Characterization of Stx And/or Eae-positive Escherichia Coli Isolated From Wildlife, Including a Typical EPEC Strain From a Wild Boar. Vet Microbiol. 2017;207:69-73. PubMed PMID: 28757042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence and characterization of stx and/or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar. AU - Alonso,Carla Andrea, AU - Mora,Azucena, AU - Díaz,Dafne, AU - Blanco,Miguel, AU - González-Barrio,David, AU - Ruiz-Fons,Francisco, AU - Simón,Carmen, AU - Blanco,Jorge, AU - Torres,Carmen, Y1 - 2017/06/01/ PY - 2017/03/24/received PY - 2017/05/27/revised PY - 2017/05/29/accepted PY - 2017/8/1/entrez PY - 2017/8/2/pubmed PY - 2018/4/14/medline KW - EPEC KW - Escherichia coli KW - STEC KW - Wildlife KW - subAB SP - 69 EP - 73 JF - Veterinary microbiology JO - Vet Microbiol VL - 207 N2 - Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains are food-borne pathogens associated with acute diarrhea. Haemolytic-uremic syndrome (HUS) is often a complication of STEC infection. In order to examine the occurrence, serotypes, virulence and antimicrobial-resistance profiles of STEC and EPEC in wildlife, 326 faecal E. coli strains from 304 clinically healthy animals were analyzed. For this approach stx1, stx2 and eae genes, as well as accessory virulence determinants (ehx, hlyA, saa, tia, bfp, subAB) were PCR-screened and sequenced. Serotyping was performed employing all available O (O1-O185) and H (H1-H56) antisera. Genetic diversity was analyzed by XbaI-PFGE and phylotyping. Thirteen STEC (4.3%) and 10 EPEC (3.3%) were identified among 12 deer, 3 mouflon, 6 wild boars and 2 birds. Nine STEC showed seropathotypes B (O145:[H28]) and C (O22:H8, O128:[H2]) associated with HUS, and D (O110:H28, O146:H21, O146:[H28], ONT:H8) associated with human diarrhea. Although most isolates harbored stx2b and stx1c variants, stx2a and stx1a (related with severe disease) were also detected. Additionally, the eae gene was present in one stx2a-positive O145:[H28] STEC from a deer and 11 STEC harbored subAB genes (mainly the subAB2 variant). EPEC isolates showed 7 different intimin variants (β1, β2, γ1, ε1, ζ1, ι1-A, κ). Interestingly, the O49:[H10] eae-κ EPEC isolated from a wild boar was bfpA-positive showing a combination of serotype/virulence profile previously detected among human clinical tEPEC. Based on present results, wild ruminants, wild boars and to a lesser extent birds would be carriers of potentially pathogenic STEC and EPEC strains. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/28757042/Occurrence_and_characterization_of_stx_and/or_eae_positive_Escherichia_coli_isolated_from_wildlife_including_a_typical_EPEC_strain_from_a_wild_boar_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(17)30377-2 DB - PRIME DP - Unbound Medicine ER -