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Unexpected effect of urate on hydrogen peroxide-induced oxidative damage in embryonic chicken cardiac cells.
Free Radic Res. 2017 Jul-Aug; 51(7-8):693-707.FR

Abstract

Uric acid (UA) is a potent scavenger of oxidants in most mammalian and avian species. The aim of this study was to obtain more comprehensive information regarding the relationship between different concentrations of UA and oxidative balance in chicken cardiac cells. First, oxidative damage parameters were measured in chicken cardiac cells treated with different concentrations of UA. UA concentrations within the normal physiological range had no effect, while treatment with a high level of UA, i.e. 1200 μM, increased the malondialdehyde (MDA) and protein carbonyl contents, decreased the superoxide dismutase (SOD) and catalase (CAT) activities, and had no effect on glutathione (GSH) in cardiac muscle cells. In addition, the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was stimulated in cells treated with 1200 μM UA. Next, the role of UA in protecting cells from oxidative damage was investigated in hydrogen peroxide (H2O2)-damaged chicken cardiac cells. Treatment with UA within the normal physiological range reduced the increased MDA and protein carbonyl contents and SOD enzymatic activity induced by H2O2 exposure to some extent and inhibited reactive oxygen species (ROS) formation, presumably as a result of the Nrf2 pathway activation in H2O2-damaged cells. By contrast, the MDA and protein carbonyl contents were increased, SOD enzymatic activity was depressed, and the Nrf2 pathway was further down-regulated in H2O2-damaged cells treated with 1200 μM UA. In conclusion, the results indicated that physiological UA concentration partially alleviated oxidative stress in chicken cardiac muscle cells treated with H2O2. However, supraphysiological UA concentrations promoted oxidative damages directly in primary cultured chicken cardiac muscle cells and aggravated oxidative stress in H2O2-damaged cells.

Authors+Show Affiliations

a College of Animal Science and Veterinary Medicine , Shandong Agricultural University , Tai'an , Shandong , PR China. b Shandong Key Laboratory of Animal Bioengineering and Disease Control and Prevention , Tai'an , Shandong , PR China.a College of Animal Science and Veterinary Medicine , Shandong Agricultural University , Tai'an , Shandong , PR China. b Shandong Key Laboratory of Animal Bioengineering and Disease Control and Prevention , Tai'an , Shandong , PR China.a College of Animal Science and Veterinary Medicine , Shandong Agricultural University , Tai'an , Shandong , PR China. b Shandong Key Laboratory of Animal Bioengineering and Disease Control and Prevention , Tai'an , Shandong , PR China.a College of Animal Science and Veterinary Medicine , Shandong Agricultural University , Tai'an , Shandong , PR China. b Shandong Key Laboratory of Animal Bioengineering and Disease Control and Prevention , Tai'an , Shandong , PR China.a College of Animal Science and Veterinary Medicine , Shandong Agricultural University , Tai'an , Shandong , PR China. b Shandong Key Laboratory of Animal Bioengineering and Disease Control and Prevention , Tai'an , Shandong , PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28758502

Citation

Sun, Xiaolong, et al. "Unexpected Effect of Urate On Hydrogen Peroxide-induced Oxidative Damage in Embryonic Chicken Cardiac Cells." Free Radical Research, vol. 51, no. 7-8, 2017, pp. 693-707.
Sun X, Jiao H, Zhao J, et al. Unexpected effect of urate on hydrogen peroxide-induced oxidative damage in embryonic chicken cardiac cells. Free Radic Res. 2017;51(7-8):693-707.
Sun, X., Jiao, H., Zhao, J., Wang, X., & Lin, H. (2017). Unexpected effect of urate on hydrogen peroxide-induced oxidative damage in embryonic chicken cardiac cells. Free Radical Research, 51(7-8), 693-707. https://doi.org/10.1080/10715762.2017.1362106
Sun X, et al. Unexpected Effect of Urate On Hydrogen Peroxide-induced Oxidative Damage in Embryonic Chicken Cardiac Cells. Free Radic Res. 2017 Jul-Aug;51(7-8):693-707. PubMed PMID: 28758502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Unexpected effect of urate on hydrogen peroxide-induced oxidative damage in embryonic chicken cardiac cells. AU - Sun,Xiaolong, AU - Jiao,Hongchao, AU - Zhao,Jingpeng, AU - Wang,Xiaojuan, AU - Lin,Hai, Y1 - 2017/08/18/ PY - 2017/8/2/pubmed PY - 2018/5/22/medline PY - 2017/8/1/entrez KW - Antioxidant enzymes KW - cardiac cell KW - hydrogen peroxide KW - urate SP - 693 EP - 707 JF - Free radical research JO - Free Radic Res VL - 51 IS - 7-8 N2 - Uric acid (UA) is a potent scavenger of oxidants in most mammalian and avian species. The aim of this study was to obtain more comprehensive information regarding the relationship between different concentrations of UA and oxidative balance in chicken cardiac cells. First, oxidative damage parameters were measured in chicken cardiac cells treated with different concentrations of UA. UA concentrations within the normal physiological range had no effect, while treatment with a high level of UA, i.e. 1200 μM, increased the malondialdehyde (MDA) and protein carbonyl contents, decreased the superoxide dismutase (SOD) and catalase (CAT) activities, and had no effect on glutathione (GSH) in cardiac muscle cells. In addition, the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was stimulated in cells treated with 1200 μM UA. Next, the role of UA in protecting cells from oxidative damage was investigated in hydrogen peroxide (H2O2)-damaged chicken cardiac cells. Treatment with UA within the normal physiological range reduced the increased MDA and protein carbonyl contents and SOD enzymatic activity induced by H2O2 exposure to some extent and inhibited reactive oxygen species (ROS) formation, presumably as a result of the Nrf2 pathway activation in H2O2-damaged cells. By contrast, the MDA and protein carbonyl contents were increased, SOD enzymatic activity was depressed, and the Nrf2 pathway was further down-regulated in H2O2-damaged cells treated with 1200 μM UA. In conclusion, the results indicated that physiological UA concentration partially alleviated oxidative stress in chicken cardiac muscle cells treated with H2O2. However, supraphysiological UA concentrations promoted oxidative damages directly in primary cultured chicken cardiac muscle cells and aggravated oxidative stress in H2O2-damaged cells. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/28758502/Unexpected_effect_of_urate_on_hydrogen_peroxide_induced_oxidative_damage_in_embryonic_chicken_cardiac_cells_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715762.2017.1362106 DB - PRIME DP - Unbound Medicine ER -