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Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway.
Int J Mol Med. 2017 Oct; 40(4):1089-1095.IJ

Abstract

Grifola frondosa, a type of food and medical fungus, has been shown to exhibit various pharmacological activities, including anticancer effects. As the most typical cancer diagnosed among female patients, breast cancer remains a huge concern threatening human health globally. In the present study, the anti-breast cancer effects of Grifola frondosa polysaccharides (GFPs) and the underlying mechanisms were investigated in MCF-7 and MDA-MB-231 cells, as well as in nude mice bearing MCF-7 tumor xenografts. GFPs exerted cytotoxic effects on the cells, as indicated by a decrease in cell viability, and an increase in the apoptototic rate, lactate dehydrogenase release and reactive oxygen species accumulation, inducing mitochondrial dysfunction. The increased expression of Bax, cleaved caspase-3 and caspase-8, and the reduced levels of B-cell lymphoma 2 (Bcl-2) and Bcl-extra large (Bcl‑xL) were observed in the cells incubated with GFPs and in the tumor tissues of the mice treated with GFPs. Moreover, the GFPs significantly suppressed the phosphorylation of AKT/glycogen synthase kinase-3β and extracellular signal-regulated kinases in a time-dependent manner. Finally, the inhibition of MCF-7 tumor xenograft growth further confirmed the anti-breast cancer effects of GFPs. All these findings revealed that GFPs induced human breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway, and provide experimental evidence to support the use of Grifola frondosa as a potential treatment for breast cancer.

Authors+Show Affiliations

Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China.Department of Biomedicine, Institute for Regeneration Medicine, Jilin University, Changchun, Jilin 130021, P.R. China.Department of Neurology, Brain Hospital of Jilin Province, Siping, Jilin 136000, P.R. China.Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China.Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China.Department of Neurology, The Second Hospital of Jilin University, Jilin University, Changchun, Jilin 130041, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28765878

Citation

Zhang, Yizhi, et al. "Grifola Frondosa Polysaccharides Induce Breast Cancer Cell Apoptosis Via the Mitochondrial-dependent Apoptotic Pathway." International Journal of Molecular Medicine, vol. 40, no. 4, 2017, pp. 1089-1095.
Zhang Y, Sun D, Meng Q, et al. Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway. Int J Mol Med. 2017;40(4):1089-1095.
Zhang, Y., Sun, D., Meng, Q., Guo, W., Chen, Q., & Zhang, Y. (2017). Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway. International Journal of Molecular Medicine, 40(4), 1089-1095. https://doi.org/10.3892/ijmm.2017.3081
Zhang Y, et al. Grifola Frondosa Polysaccharides Induce Breast Cancer Cell Apoptosis Via the Mitochondrial-dependent Apoptotic Pathway. Int J Mol Med. 2017;40(4):1089-1095. PubMed PMID: 28765878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway. AU - Zhang,Yizhi, AU - Sun,Dejun, AU - Meng,Qingjin, AU - Guo,Wanxu, AU - Chen,Qiuhui, AU - Zhang,Ying, Y1 - 2017/07/26/ PY - 2016/01/29/received PY - 2017/07/04/accepted PY - 2017/8/3/pubmed PY - 2018/5/16/medline PY - 2017/8/3/entrez SP - 1089 EP - 1095 JF - International journal of molecular medicine JO - Int J Mol Med VL - 40 IS - 4 N2 - Grifola frondosa, a type of food and medical fungus, has been shown to exhibit various pharmacological activities, including anticancer effects. As the most typical cancer diagnosed among female patients, breast cancer remains a huge concern threatening human health globally. In the present study, the anti-breast cancer effects of Grifola frondosa polysaccharides (GFPs) and the underlying mechanisms were investigated in MCF-7 and MDA-MB-231 cells, as well as in nude mice bearing MCF-7 tumor xenografts. GFPs exerted cytotoxic effects on the cells, as indicated by a decrease in cell viability, and an increase in the apoptototic rate, lactate dehydrogenase release and reactive oxygen species accumulation, inducing mitochondrial dysfunction. The increased expression of Bax, cleaved caspase-3 and caspase-8, and the reduced levels of B-cell lymphoma 2 (Bcl-2) and Bcl-extra large (Bcl‑xL) were observed in the cells incubated with GFPs and in the tumor tissues of the mice treated with GFPs. Moreover, the GFPs significantly suppressed the phosphorylation of AKT/glycogen synthase kinase-3β and extracellular signal-regulated kinases in a time-dependent manner. Finally, the inhibition of MCF-7 tumor xenograft growth further confirmed the anti-breast cancer effects of GFPs. All these findings revealed that GFPs induced human breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway, and provide experimental evidence to support the use of Grifola frondosa as a potential treatment for breast cancer. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/28765878/Grifola_frondosa_polysaccharides_induce_breast_cancer_cell_apoptosis_via_the_mitochondrial_dependent_apoptotic_pathway_ L2 - http://www.spandidos-publications.com/ijmm/40/4/1089 DB - PRIME DP - Unbound Medicine ER -