Tags

Type your tag names separated by a space and hit enter

Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae).
Nat Prod Res. 2018 Aug; 32(16):1991-1995.NP

Abstract

The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37-9.38 and 1.32-11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of C. ispahanica.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Karimi E
a Faculty of Science, Department of Biochemistry and Biophysics , Islamic Azad University, Mashhad Branch , Mashhad , Iran.
Ghorbani Nohooji M
b Medicinal Plants Research Center , Institute of Medicinal Plants, ACECR , Karaj , Iran.
Habibi M
c Faculty of Science and Engineering, Department of Biology , Gonbad Kavous University , Gonbad Kavous , Iran.
Ebrahimi M
d Faculty of Veterinary Medicine, Department of Veterinary Preclinical Sciences , Universiti Putra Malaysia , Serdang , Malaysia.
Mehrafarin A
b Medicinal Plants Research Center , Institute of Medicinal Plants, ACECR , Karaj , Iran.
Khalighi-Sigaroodi F
b Medicinal Plants Research Center , Institute of Medicinal Plants, ACECR , Karaj , Iran.

MeSH

AntioxidantsChromatography, High Pressure LiquidClematisFlavonoidsPhenolsPlant ExtractsRanunculaceaeSolvents

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28774179

Citation

Karimi, Ehsan, et al. "Antioxidant Potential Assessment of Phenolic and Flavonoid Rich Fractions of Clematis Orientalis and Clematis Ispahanica (Ranunculaceae)." Natural Product Research, vol. 32, no. 16, 2018, pp. 1991-1995.
Karimi E, Ghorbani Nohooji M, Habibi M, et al. Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae). Nat Prod Res. 2018;32(16):1991-1995.
Karimi, E., Ghorbani Nohooji, M., Habibi, M., Ebrahimi, M., Mehrafarin, A., & Khalighi-Sigaroodi, F. (2018). Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae). Natural Product Research, 32(16), 1991-1995. https://doi.org/10.1080/14786419.2017.1359171
Karimi E, et al. Antioxidant Potential Assessment of Phenolic and Flavonoid Rich Fractions of Clematis Orientalis and Clematis Ispahanica (Ranunculaceae). Nat Prod Res. 2018;32(16):1991-1995. PubMed PMID: 28774179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae). AU - Karimi,Ehsan, AU - Ghorbani Nohooji,Majid, AU - Habibi,Meisam, AU - Ebrahimi,Mahdi, AU - Mehrafarin,Ali, AU - Khalighi-Sigaroodi,Farahnaz, Y1 - 2017/08/03/ PY - 2017/8/5/pubmed PY - 2018/9/6/medline PY - 2017/8/5/entrez KW - Clematis ispahanica KW - Clematis orientalis KW - HPLC KW - antioxidant activity SP - 1991 EP - 1995 JF - Natural product research JO - Nat Prod Res VL - 32 IS - 16 N2 - The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37-9.38 and 1.32-11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 μg/mL. The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 μg/g DW) in chloroform fraction and Ellagic acid (811.7 μg/g DW) in chloroform fraction of C. ispahanica. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/28774179/Antioxidant_potential_assessment_of_phenolic_and_flavonoid_rich_fractions_of_Clematis_orientalis_and_Clematis_ispahanica__Ranunculaceae__ DB - PRIME DP - Unbound Medicine ER -