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Phytochemical investigation and nephroprotective potential of Sida cordata in rat.
BMC Complement Altern Med. 2017 Aug 04; 17(1):388.BC

Abstract

BACKGROUND

Plants are an efficient source of natural antioxidant against free radicals causing kidney damages. Sida cordata ethyl acetate fraction has been reported for strong in vitro antioxidant potency, previously. In the present study, our objective was to evaluate its in vivo antioxidant potency against CCl4 induced nephrotoxicity and investigates the bioactive phytochemicals by HPLC-DAD analysis.

METHODS

Phytochemical analysis was performed by HPLC-DAD methodology. For in vivo study, 42 male Sprague-Dawley rats were treated with alternatively managed doses for 60 days. Group I animals were remained untreated. Group II animals were treated with vehicle (1 mL of olive oil) by intragastric route on alternate days. Group III was treated with 30% CCl4 (1 mL/kg b.w.) i.p. Group IV was treated with 30% CCl4 (1 mL/kg b.w.) i.p and silymarin intragastric. Group V and VI rats were treated with 30% CCl4 and SCEE (150 and 300 mg/kg b.w., respectively) intragastric. Group VII animals were treated with SCEE (300 mg/kg b.w.) intragastrically. Blood parameters, Serum proteins and urine profile were investigated. Activities of tissue enzyme i.e. catalase, peroxidase, superoxide dismutase, glutathione-S-transferase, glutathione reductase, GSH and γ-GT were evaluated. Histopathological observations, total protein contents, lipid peroxidation, DNA damage and relative weight were also analyzed.

RESULTS

Gallic acid, catechin and caffeic acid were identified in SCEE fraction by HPLC-DAD. Decrease in the count of red blood cells, neutrophils, eosinophils and concentration of hemoglobin whereas increase in lymphocyte count and estimation of sedimentation rate (ESR) with 1 mL CCl4 (30% in Olive oil) administration (30 doses in 60 days) was restored dose dependently with co-treatment of SCEE (150 and 300 mg/kg b.w.). Treatment of rats with CCl4 markedly (P < 0.01) increased the count of urinary red blood cells and leucocytes, concentration of urea, creatinine and urobilinogen and specific gravity whereas creatinine clearance was reduced. Serum level of total protein, albumin, globulin, nitrite, creatinine and blood urea nitrogen (BUN) was significantly increased (P < 0.01) by CCl4 treatment. The activity of antioxidant enzymes; catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase and content of reduced glutathione was decreased (P < 0.01) significantly. However, increased concentration (P < 0.01) of thiobarbituric acid reactive substances and histopathological injuries were noticed in the renal tissues of rats after the treatment with CCl4. Co-administration of SCEE, dose dependently, protected the alterations in the studied parameters of rats at 150 and 300 mg/kg b.w. The present study revealed that SCEE could be used as a possible remedy for renal toxicity abnormalities.

CONCLUSION

These results are an evidence of the renal protective role of S.cordat ethyl acetate fraction against CCl4 induced nephrotoxicity in rats which may be due to its antioxidant compounds.

Authors+Show Affiliations

Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan. drnaseeralishah@gmail.com.Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, , 45320, Pakistan.Ethiopian Public Health Institute, Addis Ababa, Ethiopia. dere_nig@hotmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28778164

Citation

Shah, Naseer Ali, et al. "Phytochemical Investigation and Nephroprotective Potential of Sida Cordata in Rat." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 388.
Shah NA, Khan MR, Nigussie D. Phytochemical investigation and nephroprotective potential of Sida cordata in rat. BMC Complement Altern Med. 2017;17(1):388.
Shah, N. A., Khan, M. R., & Nigussie, D. (2017). Phytochemical investigation and nephroprotective potential of Sida cordata in rat. BMC Complementary and Alternative Medicine, 17(1), 388. https://doi.org/10.1186/s12906-017-1896-8
Shah NA, Khan MR, Nigussie D. Phytochemical Investigation and Nephroprotective Potential of Sida Cordata in Rat. BMC Complement Altern Med. 2017 Aug 4;17(1):388. PubMed PMID: 28778164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical investigation and nephroprotective potential of Sida cordata in rat. AU - Shah,Naseer Ali, AU - Khan,Muhammad Rashid, AU - Nigussie,Dereje, Y1 - 2017/08/04/ PY - 2016/12/07/received PY - 2017/07/31/accepted PY - 2017/8/6/entrez PY - 2017/8/6/pubmed PY - 2017/9/2/medline KW - Antioxidant enzymes KW - Blood KW - Cellular injuries KW - Ethyl acetate KW - Renal function test KW - S. cordata SP - 388 EP - 388 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Plants are an efficient source of natural antioxidant against free radicals causing kidney damages. Sida cordata ethyl acetate fraction has been reported for strong in vitro antioxidant potency, previously. In the present study, our objective was to evaluate its in vivo antioxidant potency against CCl4 induced nephrotoxicity and investigates the bioactive phytochemicals by HPLC-DAD analysis. METHODS: Phytochemical analysis was performed by HPLC-DAD methodology. For in vivo study, 42 male Sprague-Dawley rats were treated with alternatively managed doses for 60 days. Group I animals were remained untreated. Group II animals were treated with vehicle (1 mL of olive oil) by intragastric route on alternate days. Group III was treated with 30% CCl4 (1 mL/kg b.w.) i.p. Group IV was treated with 30% CCl4 (1 mL/kg b.w.) i.p and silymarin intragastric. Group V and VI rats were treated with 30% CCl4 and SCEE (150 and 300 mg/kg b.w., respectively) intragastric. Group VII animals were treated with SCEE (300 mg/kg b.w.) intragastrically. Blood parameters, Serum proteins and urine profile were investigated. Activities of tissue enzyme i.e. catalase, peroxidase, superoxide dismutase, glutathione-S-transferase, glutathione reductase, GSH and γ-GT were evaluated. Histopathological observations, total protein contents, lipid peroxidation, DNA damage and relative weight were also analyzed. RESULTS: Gallic acid, catechin and caffeic acid were identified in SCEE fraction by HPLC-DAD. Decrease in the count of red blood cells, neutrophils, eosinophils and concentration of hemoglobin whereas increase in lymphocyte count and estimation of sedimentation rate (ESR) with 1 mL CCl4 (30% in Olive oil) administration (30 doses in 60 days) was restored dose dependently with co-treatment of SCEE (150 and 300 mg/kg b.w.). Treatment of rats with CCl4 markedly (P < 0.01) increased the count of urinary red blood cells and leucocytes, concentration of urea, creatinine and urobilinogen and specific gravity whereas creatinine clearance was reduced. Serum level of total protein, albumin, globulin, nitrite, creatinine and blood urea nitrogen (BUN) was significantly increased (P < 0.01) by CCl4 treatment. The activity of antioxidant enzymes; catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase and content of reduced glutathione was decreased (P < 0.01) significantly. However, increased concentration (P < 0.01) of thiobarbituric acid reactive substances and histopathological injuries were noticed in the renal tissues of rats after the treatment with CCl4. Co-administration of SCEE, dose dependently, protected the alterations in the studied parameters of rats at 150 and 300 mg/kg b.w. The present study revealed that SCEE could be used as a possible remedy for renal toxicity abnormalities. CONCLUSION: These results are an evidence of the renal protective role of S.cordat ethyl acetate fraction against CCl4 induced nephrotoxicity in rats which may be due to its antioxidant compounds. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28778164/Phytochemical_investigation_and_nephroprotective_potential_of_Sida_cordata_in_rat_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1896-8 DB - PRIME DP - Unbound Medicine ER -