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Human milk oligosaccharide consumption by probiotic and human-associated bifidobacteria and lactobacilli.
J Dairy Sci. 2017 Oct; 100(10):7825-7833.JD

Abstract

Human milk contains high concentrations of nondigestible complex oligosaccharides (human milk oligosaccharides; HMO) that reach the colon and are subsequently fermented by the infant gut microbiota. Using a high-throughput, low-volume growth determination, we evaluated the ability of 12 lactobacilli and 12 bifidobacteria strains, including several commercial probiotics, to ferment HMO and their constituent monomers. Of the 24 strains tested, only Bifidobacterium longum ssp. infantis ATCC 15697 and Bifidobacterium infantis M-63 were able to ferment 3'-sialyllactose, 6'-sialyllactose, 2'-fucosyllactose, and 3'-fucosyllactose. Bifidobacterium infantis M-63 degraded almost 90% of the 2'-fucosyllactose but left most of the fucose in the supernatant, as detected by HPLC. Among bifidobacteria, only the B. infantis strains and Bifidobacterium breve ATCC 15700 were able to ferment lacto-N-neotetraose (LNnT). Among lactobacilli, Lactobacillus acidophilus NCFM was found to be the most efficient at utilizing LNnT. The extracellular β-galactosidase (lacL, LBA1467) of L. acidophilus NCFM cleaves the terminal galactose of LNnT for growth, leaving lacto-N-triose II in the media as detected by HPLC. Inactivation of lacL abolishes growth of L. acidophilus NCFM on LNnT. These results contribute to our knowledge of HMO-microbe interactions and demonstrate the potential for synbiotic combinations of pre- and probiotics.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801.Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801.Abbott Nutrition, Columbus, OH 43219.Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801. Electronic address: mille216@illinois.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28780103

Citation

Thongaram, Taksawan, et al. "Human Milk Oligosaccharide Consumption By Probiotic and Human-associated Bifidobacteria and Lactobacilli." Journal of Dairy Science, vol. 100, no. 10, 2017, pp. 7825-7833.
Thongaram T, Hoeflinger JL, Chow J, et al. Human milk oligosaccharide consumption by probiotic and human-associated bifidobacteria and lactobacilli. J Dairy Sci. 2017;100(10):7825-7833.
Thongaram, T., Hoeflinger, J. L., Chow, J., & Miller, M. J. (2017). Human milk oligosaccharide consumption by probiotic and human-associated bifidobacteria and lactobacilli. Journal of Dairy Science, 100(10), 7825-7833. https://doi.org/10.3168/jds.2017-12753
Thongaram T, et al. Human Milk Oligosaccharide Consumption By Probiotic and Human-associated Bifidobacteria and Lactobacilli. J Dairy Sci. 2017;100(10):7825-7833. PubMed PMID: 28780103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human milk oligosaccharide consumption by probiotic and human-associated bifidobacteria and lactobacilli. AU - Thongaram,Taksawan, AU - Hoeflinger,Jennifer L, AU - Chow,JoMay, AU - Miller,Michael J, Y1 - 2017/08/02/ PY - 2017/02/17/received PY - 2017/06/14/accepted PY - 2017/8/7/pubmed PY - 2018/6/6/medline PY - 2017/8/7/entrez KW - Bifidobacterium KW - Lactobacillus acidophilus NCFM KW - human milk oligosaccharide KW - probiotic SP - 7825 EP - 7833 JF - Journal of dairy science JO - J Dairy Sci VL - 100 IS - 10 N2 - Human milk contains high concentrations of nondigestible complex oligosaccharides (human milk oligosaccharides; HMO) that reach the colon and are subsequently fermented by the infant gut microbiota. Using a high-throughput, low-volume growth determination, we evaluated the ability of 12 lactobacilli and 12 bifidobacteria strains, including several commercial probiotics, to ferment HMO and their constituent monomers. Of the 24 strains tested, only Bifidobacterium longum ssp. infantis ATCC 15697 and Bifidobacterium infantis M-63 were able to ferment 3'-sialyllactose, 6'-sialyllactose, 2'-fucosyllactose, and 3'-fucosyllactose. Bifidobacterium infantis M-63 degraded almost 90% of the 2'-fucosyllactose but left most of the fucose in the supernatant, as detected by HPLC. Among bifidobacteria, only the B. infantis strains and Bifidobacterium breve ATCC 15700 were able to ferment lacto-N-neotetraose (LNnT). Among lactobacilli, Lactobacillus acidophilus NCFM was found to be the most efficient at utilizing LNnT. The extracellular β-galactosidase (lacL, LBA1467) of L. acidophilus NCFM cleaves the terminal galactose of LNnT for growth, leaving lacto-N-triose II in the media as detected by HPLC. Inactivation of lacL abolishes growth of L. acidophilus NCFM on LNnT. These results contribute to our knowledge of HMO-microbe interactions and demonstrate the potential for synbiotic combinations of pre- and probiotics. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/28780103/Human_milk_oligosaccharide_consumption_by_probiotic_and_human_associated_bifidobacteria_and_lactobacilli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(17)30730-0 DB - PRIME DP - Unbound Medicine ER -